Evaluation of imatinib mesylate (Gleevec) on  KAI1/CD82  gene expression in breast cancer MCF-7 cells using quantitative real-time PCR

Shandiz, Seyed Ataollah Sadat; Khosravani, Marjan; Mohammadi, Sepideh; Noorbazargan, Hassan; Mirzaie, Amir; Inanlou, Davoud Nouri; Jalali, Mojgan Dalirsaber; Jouzaghkar, Hamidreza; Baghbani-Arani, Fahimeh; Keshavarz-Pakseresht, Behta

doi:10.1016/j.apjtb.2015.10.006

Cited by 25 publications

(10 citation statements)

References 24 publications

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“…Imatinib mesylate (STI571; Gleevec V R ; Novartis, Basel, Switzerland) is the first successful member of a new class of kinase inhibitors that acts by inhibiting specific tyrosine kineses like Bcr-Abl fusion oncoprotein in chronic myeloid leukaemia (CML), as well as inhibits the activation of platelet-derived growth factor (PDGF)ab, colony stimulating factor 1 (CSF1) receptors and ckit, which regulates major cellular events. It is currently used in research and treatment of several solid tumors (Blay and Rutkowski 2014, Sadat Shandiz et al 2016a, 2016b, Woo et al 2015. Despite the success in tyrosine kinase inhibitors for the treatment of cancer, the bioavailability of most of the small kinase inhibitors in the physiological environment is extremely poor due to hydrophobic nature (Pawson and Warner 2007).…”

Section: Introductionmentioning

confidence: 99%

Novel imatinib-loaded silver nanoparticles for enhanced apoptosis of human breast cancer MCF-7 cells

Shandiz

Ardestani

Shahbazzadeh

et al. 2016

Artificial Cells, Nanomedicine, and Biotechnology

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In the current study, in vitro biological feature of imatinib-loaded silver nanoparticles (IMAB-AgNPs) on human breast cancer cell line was investigated. The formation of synthesized silver nanoparticles (AgNPs) was characterized by UV-Visible spectroscopy, EDS, TEM imaging, SEM, FTIR, DLS and Zeta potentiometer. The developed IMAB-AgNPs with maximum percentage of loading efficiency was demonstrated in the average of 130 nm and mostly spherical. Additionally, in vitro drug release study showed a slow and continuous release of imatinib over a period of 80 h. We demonstrated that the synthesized IMAB-AgNPs exhibited a dose-dependent cytotoxicity against MCF-7 cell line. Then, real-time PCR method was also applied for the investigation of Bax and Bcl-2 gene expression in the cells. Comparing IMAB-AgNPs to AgNPs and Imatinib revealed the ability of IMAB-AgNPs to up-regulating Bax/Bcl-2 ratio. An induction of apoptosis was evidenced by Annexin-V/PI detection assay. Based on the current obtained data, the IMAB-AgNPs can exhibit inhibitory effect on viability through up regulation of apoptosis in MCF-7 cancer cells, which provides influencing evidence for the green synthesized AgNPs as a promising sustained drug delivery system.

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Section: Introductionmentioning

confidence: 99%

Novel imatinib-loaded silver nanoparticles for enhanced apoptosis of human breast cancer MCF-7 cells

Shandiz

Ardestani

Shahbazzadeh

et al. 2016

Artificial Cells, Nanomedicine, and Biotechnology

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“…16 Several studies were designed to explore the growth inhibitory effect of IM either in vitro (cancer cell lines) or in vivo (tumor-bearing experimental animals) on variety of cancer cell types, such as of breast, 17,18 liver, 19 prostate, 20 and colorectal origin 21 as well as many other types of malignant tumors. 22,23 Besides IM great effectiveness, patients treated with IM may suffer from its side effects and long-term toxicity as renal, cardiac, and hepatotoxicity. 24,25 In relation to autophagy pathway of cancer cells, the inhibition of autophagy was proved to increase the effect of IM and thus, by using NCs with IM treatment, the autophagy hindrance effect of NCs will give a synergistic action, which can substantially reveals a potential formulation of IM in the era of cancer treatment.…”

Section: Introductionmentioning

confidence: 99%

Maximizing the Therapeutic Efficacy of Imatinib Mesylate–Loaded Niosomes on Human Colon Adenocarcinoma Using Box-Behnken Design

Kassem

El-Sawy

Abd-Allah

et al. 2017

Journal of Pharmaceutical Sciences

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This research purposed to formulate an optimized imatinib mesylate (IM)-loaded niosomes to improve its chemotherapeutic efficacy. The influence of 3 formulation factors on niosomal vesicular size (Y), zeta potential (Y), entrapment capacity percentage (Y), the percentage of initial drug release after 2 h (Y), and the percentage of cumulative drug release after 24 h (Y) were studied and optimized using Box-Behnken design. Optimum desirability was specified and the optimized formula was prepared, stability tested, morphologically examined, checked for vesicular bilayer formation and evaluated for its in vitro cytotoxicity on 3 different cancer cell lines namely MCF-7, HCT-116, and HepG-2 in addition to 1 normal cell line to ensure its selectivity against cancer cells. The actual responses of the optimized IM formulation were 425.36 nm, -62.4 mV, 82.96%, 18.93%, and 89.45% for Y, Y, Y, Y, and Y, respectively. The optimized IM-loaded niosomes confirmed the spherical vesicular shape imaged by both light and electron microscopes and further proven by differential scanning calorimetry. Moreover, the optimized formula exhibited improved stability on storage at 4 ± 2°C and superior efficacy on MCF7, HCT-116, and HepG2 as IC values were 6.7, 16.4, and 7.3 folds less than those of free drug, respectively. Interestingly, IC of the optimized formula against normal cell line was ranged from 3 to 11 folds higher than in different cancer cells indicating a higher selectivity of the optimized formula to cancer cells. In conclusion, the incorporation of IM in niosomes enhanced its efficacy and selectivity toward cancer cells, presenting a promising tool to fight cancer using this approach.

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“…Subsequently, the resulting purple formazan crystals were dissolved in dimethyl sulfoxide and then the optical density (OD) of each well was measured. 31 The absorbance at 570 nm was evaluated using a microplate reader (Bio-Rad 680; Microplate Master, Hercules, CA, USA). The effect of NPs on the cells was expressed as the percentage of cell viability compared to control, which was calculated according to the following formula: percentage of cell viability (%) = mean OD/control OD ×100.…”

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confidence: 99%

Phytosynthesis of silver nanoparticles using Artemisia marschalliana Sprengel aerial part extract and assessment of their antioxidant, anticancer, and antibacterial properties

Salehi

Shandiz

Ghanbar

et al. 2016

IJN

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A rapid phytosynthesis of silver nanoparticles (AgNPs) using an extract from the aerial parts of Artemisia marschalliana Sprengel was investigated in this study. The synthesized AgNPs using A. marschalliana extract was analyzed by UV–visible spectroscopy, X-ray diffraction, and Fourier transform infrared spectroscopy and further characterized by transmission electron microscopy, scanning electron microscopy, zeta potential, and energy-dispersive spectroscopy. Characteristic absorption bands of AgNPs were found near 430 nm in the UV–vis spectrum. Energy-dispersive spectroscopy analysis of AgNPs in the energy range 2–4 keV confirmed the silver signal due to surface plasmon resonance. Scanning electron microscopy and transmission electron microscopy results revealed that the AgNPs were mostly spherical with an average size ranging from 5 nm to 50 nm. The zeta potential value of −31 mV confirmed the stability of the AgNPs. AgNPs produced using the aqueous A. marschalliana extract might serve as a potent in vitro antioxidant, as revealed by 2,2-diphenyl-1-picryl hydrazyl assay. The present study demonstrates the anticancer properties of phytosynthesized AgNPs against human gastric carcinoma AGS cells. AgNPs exerted a dose-dependent inhibitory effect on the viability of cells. Real-time polymerase chain reaction was used for the investigation of Bax and Bcl-2 gene expression in cancer and normal cell lines. Our findings show that the mRNA levels of pro-apoptotic Bax gene expression were significantly upregulated, while the expression of anti-apoptotic Bcl-2 was declined in cells treated with AgNPs compared to normal cells. In addition, flow cytometric analysis showed that the number of early and late apoptotic AGS cells was significantly enhanced following treatment with AgNPs as compared to untreated cells. In addition, the AgNPs showed strong antibacterial properties against tested pathogenic bacteria such as Staphylococcus aureus , Bacillus cereus , Acinetobacter baumannii , and Pseudomonas aeruginosa . Based on the obtained data, we suggest that phytosynthesized AgNPs are good alternatives in the treatment of diseases because of the presence of bioactive agents.

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Evaluation of imatinib mesylate (Gleevec) on KAI1/CD82 gene expression in breast cancer MCF-7 cells using quantitative real-time PCR

Cited by 25 publications

References 24 publications

Novel imatinib-loaded silver nanoparticles for enhanced apoptosis of human breast cancer MCF-7 cells

Novel imatinib-loaded silver nanoparticles for enhanced apoptosis of human breast cancer MCF-7 cells

Maximizing the Therapeutic Efficacy of Imatinib Mesylate–Loaded Niosomes on Human Colon Adenocarcinoma Using Box-Behnken Design

Phytosynthesis of silver nanoparticles using Artemisia marschalliana Sprengel aerial part extract and assessment of their antioxidant, anticancer, and antibacterial properties

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