Evaluation of IgM Lateral Flow Assay as a Screening Tool for
            <i>Mycoplasma pneumoniae</i>
            Infection in Childhood Pneumonia

Sauteur, Patrick M. Meyer; Pánisová, Elena; Bachmann, Lucas M.; Ambroggio, Lilliam; Berger, Christoph

doi:10.1128/jcm.01498-20

Cited by 4 publications

(5 citation statements)

References 9 publications

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“…The limitation of paired sera IgG test is that it can be only used for retrospective diagnosis and could not be applied for the early diagnosis. The long-time existence of IgM in the body can result in false positivity, and IgM serology may lead to false positive due to limited assay performance and age/ host-dependent characteristics or false-negative results early in disease course and after reinfection (72). Chang et al demonstrated that IgM showed poor sensitivity and positive predictive value, so the interpretation of IgM should be done with caution (73).…”

Section: Mrmp Diagnosismentioning

confidence: 99%

The molecular characteristics, diagnosis, and treatment of macrolide-resistant Mycoplasma pneumoniae in children

2023

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The purpose of this study is to review the molecular characteristics, the diagnosis, and treatment of the widespread infection of macrolide-resistant Mycoplasma pneumoniae (M. pneumoniae; MRMP) in children, thus providing a better knowledge of this infection and presenting the associated problems. Single point mutations in the V region of the 23S rRNA gene of M. pneumoniae genome are associated with macrolide resistance. P1–1, MLVA4-5-7-2, and ST3 are usually the predominated genetic types in the M. pneumoniae epidemics. The short-term two times serological IgM (or together with IgG) test in the acute stage can be used for confirmation. Combined serological testing and PCR might be a more prudent method to reduce macrolide consumption and antibiotic selective pressure in a clinical setting. Molecular methods for the detection of single-nucleotide mutations in the V region of the 23S rRNA gene can be used for the diagnosis of MRMP. The routine use of macrolide for the treatment of macrolide-sensitive Mycoplasma pneumoniae (MSMP) infections can get good effect, but the effects are limited for severe MRMP infections. Additional corticosteroids may be required for the treatment of severe MRMP infections in children in China during the era of MRMP.

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Section: Mrmp Diagnosismentioning

confidence: 99%

The molecular characteristics, diagnosis, and treatment of macrolide-resistant Mycoplasma pneumoniae in children

2023

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“…Immunoglobulins are considered potent immunomodulators for various immune-mediated diseases, and it has marked activity against Mycoplasma pneumonia and provide additional protection, especially in patients who do not respond well to antibiotic therapy. Immunoglobulins have been reported to be effective in the treatment of many autoimmune and systemic inflammatory diseases [18][19][20]. Immunoglobulins have significant effects on different immune system effector cells (such as B and T lymphocytes and dendritic cells) and regulate a wide range of genes.…”

mentioning

confidence: 99%

Efficacy of Gamma Globulin Combined with Azithromycin Sequential Therapy in the Treatment of RMPP and Its Effect on Th1/Th2 Cytokine Levels

Jia

Tian

et al. 2022

Computational and Mathematical Methods in Medicine

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Objective. To investigate the efficacy of gamma globulin combined with azithromycin sequential therapy in the treatment of children with refractory mycoplasma pneumonia and its effect on Th1/Th2 cytokine levels. Method. From January 2021 to January 2022, 100 children diagnosed with refractory mycoplasma pneumonia were randomly divided into 2 groups (50 cases in each one), the control group was treated with azithromycin plus comprehensive basic treatment, and the treatment group was treated with combined treatment on the basis of the control group, gamma globulin therapy; the treatment effect and cytokine levels of the two groups were compared. Results. Th1, Th2, and Th1/Th2 before treatment were not significantly different between the two groups. Th1, Th2, and Th1/Th2 in the treatment group were significantly downregulated compared with those in the control group after treatment. The levels of IgG, IgA, and IgM in the treatment group were not significantly different from those in the control group before treatment but were significantly upregulated after treatment. IL-10, IL-6, and IL-2 levels were also significantly increased in the treatment group. The disappearance time of clinical symptoms such as fever, cough, and pulmonary rales in the treatment group was significantly shorter than that in the control group, and the cure rate in the treatment group was significantly better than that in the control group. Conclusion. The clinical effect of gamma globulin combined with azithromycin sequential therapy in the treatment of children with refractory mycoplasma pneumonia is remarkable, which can reduce inflammatory factors, improve patients’ immunity, and promote disease recovery.

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“…In the study of Gill et al [10] the authors evaluate the performance of the commercially and no point-of-care (POC) test is available for direct M. pneumoniae detection. Meyer-Sauteur et al [6] The microbiological diagnosis of Pneumocystis jirovecii pneumonia (PCP) is difficult since the P. jirovecii load in the lungs is, in general, low in non-HIV-positive patients. Currently, the laboratory gold standard for the detection of P. jirovecii is a real-time quantitative PCR (qPCR) assay, however, this is laborious, require skilled personnel, and execution outside regular working hours of the molecular biology laboratory is limited.…”

Section: Detection Of Resistance To Antimicrobialsmentioning

confidence: 99%

“…The measurement of M. pneumoniae -specific IgM antibody secreting cells (ASC) by enzyme-linked immunospot (ELISpot) assay differentiates between M. pneumoniae infection and carriage, but this assay cannot be performed at bedside and no point-of-care (POC) test is available for direct M. pneumoniae detection. Meyer-Sauteur et al [ 6 ] assessed a new immunochromatographic POC IgM lateral flow assay (LFA) as an on-site screening tool for the detection of M. pneumoniae CAP in children. A set of 239 pediatric serum samples from 94 CAP patients and 145 healthy controls was used from a previous study.…”

Section: Diagnostic Microbiological Techniquesmentioning

confidence: 99%

What happened to microbiological diagnosis in 2020 beyond COVID-19?

Cercenado

2021

Rev Esp Quimioter

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The global pandemic of COVID-19 has had negative repercussions on the activities and research in clinical microbiology laboratories other than those related to SARS-CoV-2. Nonetheless, the research activity has also continued in other fields. In this brief review, some of the recent publications related to new diagnostic tests, methods for rapid antimicrobial susceptibility testing and for the detection of resistance genes, new diagnostic technologies, and some aspects related to old and emergent pathogens (Candida auris, Elizabethkingia spp. Streptococcus pyogenes) are summarized.

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Evaluation of IgM Lateral Flow Assay as a Screening Tool for Mycoplasma pneumoniae Infection in Childhood Pneumonia

Abstract: Diagnosis of Mycoplasma pneumoniae (Mp) infection with community-acquired pneumonia (CAP) is challenging (1).…

Cited by 4 publications

References 9 publications

The molecular characteristics, diagnosis, and treatment of macrolide-resistant Mycoplasma pneumoniae in children

The molecular characteristics, diagnosis, and treatment of macrolide-resistant Mycoplasma pneumoniae in children

Efficacy of Gamma Globulin Combined with Azithromycin Sequential Therapy in the Treatment of RMPP and Its Effect on Th1/Th2 Cytokine Levels

What happened to microbiological diagnosis in 2020 beyond COVID-19?

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