Evaluation of in vivo biocompatibility and biodegradation of photocrosslinked hyaluronate hydrogels (HADgels)

Abstract

HADgels are newly developed photocrosslinked hyaluronate hydrogels. They are produced from an aqueous solution of a hyaluronan derivative (HAD) in which cinnamic acid is introduced into the carboxyl moiety of hyaluronan using 3-aminopropanol as a spacer. High-energy ultraviolet irradiation of the HAD solution induces photodimerization of cinnamic acid, resulting in the development of a macromolecular network of each hyaluronan to water-insoluble hydrogels. The biocompatibility and biodegradation of HADgels were evaluated by guinea pig intracutaneous injection testing for up to 28 days. By macroscopic and histological observations, HADgels showed good tissue compatibility and did not induce excess inflammation at the injection sites. Biodegradation of the HADgels clearly depended on the degree of crosslinking at the fixed weight concentrations of HAD (0.5% and 1.0%). In addition, serum analyses showed that the injected guinea pigs did not produce specific antibodies against HADgels. These results indicate that HADgels have preferable biocompatibility and can be used as a new class of injectable, absorbable biomaterial, especially for preventing postsurgical adhesion formations.

Development of a novel injectable drug delivery system for subconjunctival glaucoma treatment

“…Previously, the biocompatibility of LDD components has been separately demonstrated for both, HA and ELA-NCO [19,41]. Among glaucoma drugs, LA was chosen for drug release experiments, because, as already mentioned, the current literature lacks any data concerning long-term subconjunctival LA delivery.…”

Visible light-induced crosslinkable gelatin

“…Some types of ultraviolet-crosslinkable biomacromolecules have also been reported [17][18][19][20][21]. Recently, visible light-crosslinkable gelatin [21] and fibrinogen [22] have been reported.…”

Pseudomonas putida as a platform for the synthesis of aromatic compounds

“…Trans-cinnamic acid is an aromatic compound that is used in the manufacture of flavours (as precursor of methyl, ethyl and benzyl esters for perfumes), dyes and pharmaceuticals, as well as a precursor of the sweetener aspartame. Although trans-cinnamate is produced in nature by plants and fungal species (Cochrane et al, 2004;Nijkamp et al, 2005), its current bulk production is via chemical synthesis (Burt, 2004;Miyamoto et al, 2004;Nijkamp et al, 2005;Noda et al, 2011). Biological approaches have also been performed by using phenylalanine ammonia lyase (PAL) enzyme genes from different microorganisms (Nijkamp et al, 2005;Noda et al, 2011).…”