Evaluation of In Vitro Invertase Inhibitory Activity of M anilkara zapota Seeds - A Novel Strategy to Manage Diabetes Mellitus

Abstract

Artocarpus heterophyllus (jack fruit), Manilkara zapota (sapota), Mangifera indica (mango), Vitis vinifera (grapes), Citrus sinensis (orange) and Syzygium cumini (jambul) seeds were selected to evaluate the in vitro invertase inhibitory activity. M. zapota (shake flask method: 98.7%) and A. heterophyllus (shake flask method: 35.6%) seeds recorded the highest and lowest in vitro invertase inhibitory activity, respectively. The two-dimensional thin-layer chromatography and twodimensional preparative thin-layer chromatography (2D PTLC) analyses have demonstrated the presence of polyphenols in M. zapota and A. heterophyllus seeds. The 2D PTLC eluate of M. zapota has recorded a significant invertase inhibitory activity. Liquid chromatography-mass spectrometry photo diode array analysis of M. zapota has exhibited the presence of four different polyphenols such as benzoyl hexosyl methyl luteolin sulfate (m/z 662.1), 6C/8C hexosyl, 6C/8C pentosyl apigenin (m/z 568), one unknown carboxylated flavonol glycoside (m/z 484.7) and one unknown flavone derivative (m/z 473.8). PRACTICAL APPLICATIONSThe fruits of Manilkara zapota and Artocarpus heterophyllus have been consumed for their sweet delicious nature by most Asian community. Ethnobotanically, many parts of the above said species have been taken orally to treat various ailments. In the current scenario, appropriate food formulation as a health mix may be tried along with the seeds of M. zapota and/or A. heterophyllus to have a balanced glycemic control in the biological system.

UPLC-QTOF-MS/MS analysis and antibacterial activity of the Manilkara zapota (L.) P. Royen against Escherichia coli and other MDR bacteria

“…Manilkara zapota (L.) P Royen is a plant present in tropical regions, originating in Mexico, whose fruits are used in food (11), with recognized medicinal properties (12). Its anti-cancer (13), anti-hyperglycemic (14), antioxidant (15), anti-inflammatory (16,17), antifungal (18) and antibacterial effects (19) are among its previously cited properties in the literature.…”

Purified novel and new diferuloyl glycerate related phenolic acid from Pandanus odoratissimus flowers shows antioxidant, invertase inhibition and control against diabetic foot ulcer (DFU) causing bacterial pathogens - An in vitro study to establish an effective regulation over type 2 diabetes mellitus

“…About 25 μl of the methanolic extract was spotted and the chromatogram was developed one dimensionally in an air-tight chamber in the presence of mobile phase consists of ethyl acetate -ethanol -water (5:1:5). The plates were then visualized under far UV light (360 nm) to detect the phenolics present in extracts (Sathishkumar et al, 2015).…”

“…First dimension: Ethyl acetate -ethanol -water (5:1:5) Second dimension: Acetone: acetic acid (17:3) Page 3/11 Therapeutic properties of purified diferuloyl glycerate from Pandanus odoratissimus flowers After the development of chromatogram, the visualized strong spots were carefully eluted and suspended in phosphate buffer (pH 7.0), and centrifuged at 10,000 rpm for 10 minutes. The resultant supernatant was lyophilized and subjected for experimental analysis (Sathishkumar et al, 2015).…”