Evaluation ofIn VitroAntioxidant Activity ofSida rhombifolia(L.) Ssp.retusa(L.)

Abstract

Sida rhombifolia (L.) ssp. retusa (L.) is widely used in Ayurvedic medicine for the treatment of fever as well as a diuretic. The comparative antioxidant potentials of ethanol extract of roots, stems, leaves, and whole plant were studied. Estimation of total polyphenolic content and high-performance thin-layer chromatography profile were determined. Further inhibition of oxygen-derived free radicals, viz., assays for free radical scavenging, reducing power, superoxide anion scavenging, nitric oxide scavenging, and anti-lipid peroxidation, were performed. All the antioxidant activities were compared with standard antioxidants such as butylated hydroxyanisole and alpha-tocopherol acetate. Extracts were found to be good scavengers of the 1,1-diphenyl-2-picrylhydrazyl radical in the order root > leaves > whole plant > stem with 50% inhibitory concentrations of 546.1, 852.8, 983.8, and 1,222.5 microg/mL, respectively. All extracts of this plant showed effective free radical scavenging activity, reducing power, and superoxide scavenging activity. Only root extract inhibited lipid peroxidation in rat liver and brain homogenate. All these antioxidant properties were concentration dependent. In addition, total polyphenolic contents of all the extracts were determined as gallic acid equivalents. The highest antioxidant activity was observed in root extract. The results obtained from the current study indicate that S. rhombifolia ssp. retusa is a potential source of natural antioxidants.

Evaluation of Antibacterial Activities of Compounds Isolated From Sida rhombifolia Linn. (Malvaceae)

“…An in vitro study using ethanol extract of roots, stems, leaves, and whole plant showed antioxidant activities. This indicated that Sida rhombifolia could be promising a source of natural antioxidants [25,26]. Similar results were also reported recently using root and stem extracts on animal models [27].…”

Ecdysteroids in Sida tuberculata R.E. Fries (Malvaceae): Chemical composition by LC–ESI-MS and selective anti-Candida krusei activity

“…As is well known, these substances interfere in several biological systems of mammals, mainly due their known antioxidant potential with hydrogen-donating radical scavenger properties (Urquiaga & Leighton, 2000). In S. rhombifolia, the total phenolics and flavonoids are reported in extracts obtained by maceration and decoction using water and hydroethanolic solutions; roots, stem and leaves have also been studied (Dhalwal, Deshpande, & Purohit, 2007;Thounaojam et al, 2011).…”

Antityphoid and radical scavenging properties of the methanol extracts and compounds from the aerial part of Paullinia pinnata

“…Thus, if the bacteria are prevented from producing reactive oxygen compounds, it can contribute to efficiently fight against the microorganism. When bacteria interact with a eukaryotic host, large quantities of reactive oxygen intermediates are produced (Kamlesh et al 2007) by phagocytes during uptake of microorganisms, and this is a major microbicidal effector mechanism against pathogenic bacteria. Reactive oxygen species can lead to serious health problems including sickle cell diseases, artherosclerosis, Parkinson’s disease, heart failure, myocardial infarction, Alzheimer’s disease, Schizophrenia, and chronic fatigue syndrom (De Diego-Otero et al 2009).…”