Evaluation ofenset(Ensete ventricosum) clones for resistance reaction against pathogenicXanthomonas campestris pv. musacearumisolates from Southwestern Ethiopia

Abstract

Enset (Ensete ventricosum) bacterial wilt (EBW) incited by Xanthomonas campestris pv. musacearum (Xcm) is threatening enset production in southwestern Ethiopia. The objectives of this study were to determine the pathogenicity of Xcm isolates and select enset clones resistant to pathogenic Xcm isolates in the study areas. A total of 30 Xcm isolates were subjected to pathogenicity tests on a susceptible enset clone Yeko and all were found pathogenic. Out of 30 pathogenic isolates, three isolates representing three altitude groups [lowland (1470 m.a.s.l), midland (1938 m.a.s.l) and highland (2360 m.a.s.l)] were used for enset clonal evaluation trials. In the clone evaluation trial, 15 enset clones (13 local and a tolerant and susceptible check) were evaluated for 2 years (2017 and 2018) under screen house conditions at Tepi National Spice Research Center, southwestern Ethiopia. The experiments were factorially arranged in a completely randomized design with three replications. An aliquot of 10 ml of the bacterial cell suspension with a concentration of 1 × 10 8 cfu/ml was inoculated into the second innermost leaf petiole of enset using a sterile hypodermic syringe. Starting from 15 days after inoculation (DAI), data were collected on

Enset (Enset ventricosum (Welw.) Cheesman) Breeding for Various Purposes - A Review

“…Over all the, the enset clones vary in reaction bacterial wilt disease (Hunduma et al, 2015). Haile et al, (2020) conducted trial to determine the pathogenicity of Xcm isolates to select resistance/tolerant enset clones. About 30 Xcm isolates were subjected to pathogenicity test on susceptible enset clones called Yeko.…”

“…The disease progressive curve of Nobo enset clone resulted in none progression and lied on X-axis for each isolate combinations. The disease progressive Curve of enset clones Gudiro x Xcm isolates progressed slowly and decreased rapidly and declined to the X-axis in response to recovery of infestation late in the epidemic period (Haile et al, 2020). Haile et al, (2020) reported that, even though all of the Xcm isolate are pathogenic to most of the agro-ecologically adapted enset clones in South-west Ethiopia, some accessions like Gudiro, Maziya, and Nobo were showed better resistance reaction to enset bacterial wilt.…”

“…The disease progressive Curve of enset clones Gudiro x Xcm isolates progressed slowly and decreased rapidly and declined to the X-axis in response to recovery of infestation late in the epidemic period (Haile et al, 2020). Haile et al, (2020) reported that, even though all of the Xcm isolate are pathogenic to most of the agro-ecologically adapted enset clones in South-west Ethiopia, some accessions like Gudiro, Maziya, and Nobo were showed better resistance reaction to enset bacterial wilt. Therefore Gudiro and Nobo should be considered as resistant clones to the Pathogen wilt and recommended for farther bacterial wilt management systems.…”

“…Enset bacterial wilt (Xanthomonas campestris pv. musacearum) disease progress curves as affected by enset clone x isolate interaction under screen house conditions in southwestern Ethiopia Source:Haile et al, (2020)…”

Evaluation of 20 enset (Ensete ventricosum) landraces for response to Xanthomonas vasicola pv. Musacearum infection

“…Despite this, identification of resistant landraces has been challenging. Enset landraces with lower but varying susceptibility to Xvm infection (Ashagari, 1985;Archido & Tezera, 1993;Handoro & Michael, 2007;Welde-Michael et al, 2008;Tessera et al, 2008;Haile et al, 2014Haile et al, , 2020Hunduma et al, 2015;Wolde et al, 2016;Handoro & Said, 2016) have been reported. However, inconsistency in response to Xvm has been observed (Ashagari, 1985;Handoro & Michael, 2007;Welde-Michael et al, 2008;Tessera et al, 2008), potentially due to variation among batches of Xvm inoculum and its storage.…”