Evaluation of hypoglycemic and hypolipidemic activity of methanol extract of Brassica oleracea

Assad, Tahira; Khan, Rafeeq Alam; Feroz, Zeeshan

doi:10.1016/s1875-5364(14)60099-6

Cited by 21 publications

(15 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[10] Medicinal plants may act through a range of mechanisms; therefore, the present study was performed for an assessment of antihyperglycemic activity and its mechanisms of action of APME. This work is the first of its kind for the demonstration of APME antidiabetic mechanisms of action.…”

Section: Discussionmentioning

confidence: 99%

Antihyperglycemic and insulin secretagogue activities of Abrus precatorius leaf extract

Balekari¹,

Veeresham²

2016

Phcog Res

View full text Add to dashboard Cite

Aim:Abrus precatorius leaves methanolic extract (APME) was evaluated for in vivo antihyperglycemic activity and in vitro insulinotropic effect.Materials and Methods:In vivo antihyperglycemic and insulin secretagogue activities were assessed in streptozotocin-induced diabetic rats by oral administration of APME (200 mg/kg body weight [bw]) for 28 days. In vitro insulin secretion mechanisms were studied using mouse insulinoma beta cells (MIN6-β). In vivo body weight and blood glucose and in vivo and in vitro insulin levels were estimated.Results:In diabetic rats, APME treatment significantly restored body weight (26.39%), blood glucose (32.39%), and insulin levels (73.95%) in comparison to diabetic control rats. In MIN6-β cells, APME potentiated insulin secretion in a dependent manner of glucose (3–16.7 mM) and extract (5–500 μg/mL) concentration. Insulin secretagogue effect was demonstrated in the presence of 3-isobutyl-1-methyl xanthine, glibenclamide, elevated extracellular calcium, and K+ depolarized media. Insulin release was reduced in the presence of nifedipine, ethylene glycol tetra acetic acid (calcium blocking agents), and diazoxide (potassium channel opener).Conclusion:The study suggests that APME antihyperglycemic activity might involve the insulin secretagogue effect by pancreatic beta cells physiological pathways via K+-ATP channel dependent and independently, along with an effect on Ca2+ channels.SUMMARY Abrus precatorius leaves methanolic extract (APME) showed a significant anti hyperglycemic and insulin secretagogue activities in streptozotocin induced diabetic rats. Also demonstrated a potent In vitro insulin secretagogue effect in mouse insulinoma beta cells (MIN6-β)APME treatment significantly restored body weight (26.39%), reduced blood glucose (32.39%) and enhanced circulatory insulin levels (73.95%) in diabetic ratsAPME demonstrated glucose and extract dose dependent insulin secretionInsulin secretagogue effect was demonstrated in the presence of 3-isobutyl-1-methyl xanthine, glibenclamide, elevated extracellular calcium and K+ depolarized media. Insulin release was reduced in the presence of nifedipine and ethylene glycol tetra acetic acid (Calcium blocking agents), diazoxide (potassium channel opener)The insulinotropic effect of APME involves a physiologic effect on K+-ATP channel and Ca2+ channels Abbreviations Used: ANOVA: Analysis of variance, CMC: Carboxy methyl cellulose, cAMP: Cyclic adenosine monophosphate, CaCl: Calcium chloride, AP: Abrus precatorius, APME: Abrus precatorius methanolic extract, DMEM: Dulbecco's modified Eagle's medium, DMSO: Dimethyl sulphoxide, EGTA: Ethylene glycol tetra acetic acid, FCS: Fetal calf serum, IBMX: 3-Isobutyl-1-methylxanthine, KCl: Potassium chloride, KRB: Kreb's Ringer buffer, MIN6: Mouse insulinoma cell line, MTT: 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-Diphenyltetrazolium Bromide, STZ: Streptozotocin.

show abstract

Section: Discussionmentioning

confidence: 99%

Antihyperglycemic and insulin secretagogue activities of Abrus precatorius leaf extract

Balekari¹,

Veeresham²

2016

Phcog Res

View full text Add to dashboard Cite

show abstract

“…The nutritional transition towards junk foods has become the foremost reason for dramatic prevalence of obesity, coronary heart disease (CHD) and cancer [1][2][3]. The consumption of junk food or hypercaloric foods increases lipid load in cells of the body or may lead to augmented generation of Reactive Oxygen Species/Metabolites (ROS/ ROM) i.e.…”

Section: Introductionmentioning

confidence: 99%

“…The healthy dietary interventions provide sufficient amounts of exogenous antioxidants that strengthen endogenous antioxidants ultimately improve natural defense against numerous ailments [10,11]. Large population survey in China has inversely associated the sufficient intakes of vegetables and legumes with diseases [2,6,12]. Amongst various vegetables, brassica vegetables such as broccoli, cabbage, cauliflower and brussels sprouts contribute positively towards health perspectives [10,13,14].…”

Section: Introductionmentioning

confidence: 99%

Bioefficacy of red cabbage against hypercholesterolemic diet mediated oxidative stress

et al. 2019

View full text Add to dashboard Cite

Background: The shift towards hypercaloric diets and sedentariness has raised lifestyle related disorders and escalated health care cost. In order to tackle this epidemiological transition, healthy, affordable food choices should be introduced in the routine menu. In this context, red cabbage is known for its rich phytochemistry, minerals, antioxidant vitamins and dietary fiber. Considering these evidences, red cabbage leaves and its extract were assessed against hypercholesterolemia and associated oxidative stress. Methods: In bioefficacy assessment trial (12 weeks), there were two dietary regimens; normal and hypercholesterolemic (1% cholesterol) that were further split into three groups each. In both feeding trials, red cabbage leaves (20%) and its extract in dose equivalent to red cabbage leaves were assessed against control diets. At termination of trial, serum lipidemic parameters and oxidative stress biomarkers were assessed to test the efficacy of diets. Results: In hypercholesterolemic rabbits, red cabbage leaves showed significant reduction in cholesterol, LDL-c and triacylglycerol levels i.e. 15.19, 18.09 and 9.42% than extract administered groups; 10.79, 12.24 and 5.72, respectively. Besides, red cabbage leaves also portrayed momentous enhancement of superoxide dismutase (SOD) and catalase (CAT) activity up to 13.29 & 17.63% by lowering lipid peroxidation by 27.86% in hypercholesterolemic diet fed groups, whereas red cabbage extract administered group depicted relatively lesser amelioration in lipid peroxidation i.e. 21.42%. Conclusions: Red cabbage leaves possess higher ameliorative potential against altered lipidemic profile and lipid peroxidation as compared to its extract thus explains its ability to prevent exhaustion of endogenous antioxidant enzymes; SOD and CAT.

show abstract

“…Earlier surveys have inversely associated the sufficient intakes of vegetables and legumes with diseases owing to the presence of biologically active ingredients including dietary fiber, phytoceutics and minerals that aid in preventing uncontrolled free radicals (Hussein, 2012;Assad et al, 2014;Ji et al, 2015;Ashraf et al, 2016;Raza et al, 2017). Previously, these dietary approaches have also shown their defensive potential against oncogenic events.…”

Section: Introductionmentioning

confidence: 99%

“…native to southern Europe where it is being employed in numerous cuisines. It possesses crunchiness along with sweet and peppery taste; however, for optimum organoleptic features, it is cultivated in cold environments (Draghici et al, 2013;Assad et al, 2014). Recently, crucifers have gained renewed interest with special reference to ample amount of minerals such as K, Fe, Mg and Mn; involve in cardiac health and co-factor of Mn-SOD i.e.…”

Section: Introductionmentioning

confidence: 99%

Compositional Analysis of Pakistani Green and Red Cabbage

Ashfaq¹

2018

PAKJAS

View full text Add to dashboard Cite

Evaluation of hypoglycemic and hypolipidemic activity of methanol extract of Brassica oleracea

Cited by 21 publications

References 29 publications

Antihyperglycemic and insulin secretagogue activities of Abrus precatorius leaf extract

Antihyperglycemic and insulin secretagogue activities of Abrus precatorius leaf extract

Bioefficacy of red cabbage against hypercholesterolemic diet mediated oxidative stress

Compositional Analysis of Pakistani Green and Red Cabbage

Contact Info

Product

Resources

About