Evaluation of Hydrogels Based on Poloxamer 407 and Polyacrylic Acids for Enhanced Topical Activity of Gentamicin against Susceptible Infections

Nnamani, PO; Kenechukwu, FC; Anugwolu, CL; Attama, AA

doi:10.4314/tjpr.v13i9.2

Cited by 16 publications

(19 citation statements)

References 16 publications

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“…The reaction of the amine group in the aminoglycoside with the O-Phthalaldehyde in the presence of NaC as a nucleophile to yield a fluorescent isoindole which is measured at 332 nm absorbance. 12 This method is superior to that used by Nnamani et al 1 , where mercaptoethanol, which emits a characteristic unpleasant odour during the derivatization process.…”

Section: Drug Content Determinationmentioning

confidence: 90%

“…When required for topical administration, it is usually formulated as creams as well as ointments which possess various disadvantages in terms of reduced stability, erratic drug release and decreased skin permeability when compared to hydrogels. 1 The water holding capacity and permeability are the most important characteristic features of a hydrogel. 2 Biocompatibility is the third most important characteristic property required by the hydrogel as it calls for compatibility of the gel with human natural tissue without causing any toxicity upon its degradation.…”

Section: Introductionmentioning

confidence: 99%

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Formulation and optimization of gentamicin hydrogel infused with tetracarpidium conophorum extract via Central composite design for topical delivery

Ilomuanya

Amenaghawon

Odimegwu

et al. 2018

tjps

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ÖZAmaç: İstatistiksel olarak tasarlanmış deney ile birlikte Yüzey cevap grafiği metodolojisinin çok değişkenli prosesleri optimize etmede çok yararlı olduğu bulunmuştur. Bu çalışmanın amacı, iki bağımsız değişken olan permeabilite arttırıcılar/antioksidanlar (Transcutol ve Tetracarpidium conophorum EETC'nin etanolik ekstraktı) ve karıştırma oranının gentamisin hidrojelin akı ve permeasyonu üzerine olan etkisini değerlendirmektir. Gereç ve Yöntemler: Gentamisin hidrojelin formüle edilmesi için bir serbest radikal başlangıç polimerizasyon modifikasyonu kullanıldı. Permeabilite arttırıcı Transcutol: EETC (X1), Karıştırma hızı (X2) bağımsız değişkenlerinin etkisini araştırmak için bağımlı değişkenler Akı (Y1) ve 12 saatten sonra etken maddenin ex-vivo permeasyon miktarı açısından değerlendirilen 14 formülasyon serisi ile 32 faktöryel santral kompozit tasarım (CCD) kullanıldı. Introduction:Response surface methodology coupled with statistically designed experiment has been found to be very useful in optimising multivariable processes. The aim of this study is to evaluate the influence of two independent variables, a ratio of permeation enhancers/antioxidants (Transcutol and ethanolic extract of Tetracarpidium conophorum EETC) and stirring rate, on the flux and permeation of gentamicin hydrogel. Methods: A modification of free radical initial polymerization was utilized to formulate the gentamicin hydrogel. A 3² factorial central composite design was then utilized to investigate the effect of independent variables of permeation enhancer Transcutol: EETC (X1), Stirring speed (X2) via 14 formulation batches which were evaluated for dependent variables Flux (Y1) and amount of drug permeated after 12 hours (Y2) ex vivo. Results:The results of analysis of variance carried out to determine the fit of the models revealed that the models were statistically significant (p < 0.05) and did not show lack of fit (R2 > 0.80). The regression equation generated for flux was Y1 = 19.35 -25.82X1 -0.044X2 + 0.0097X1X2 + 11.86X21 and for cumulative permeation of gentamicin in 12 hours Y2= 315.50 -189.67X1 + 0.28X2 -1.29X1X2 + 123.55X21. The validity of the statistical models used for predicting flux and drug permeation was confirmed by carrying out three confirmation experimental runs at the identified optimum conditions. The results showed that there was no significant difference between the experimental results and those predicted by the statistical models. Discussion And Conclusion: The excellent correlation between the predicted and measured values shows the validity of statistical models (R2= 0.95). Utilization of antioxidant and permeation enhancer has been used for the first time to investigate the influence on dependent variables. Optimization of gentamicin hydrogel using central composite statistical design is valid for prediction of drug permeation and flux utilizing variables in formulation.

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Section: Drug Content Determinationmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Formulation and optimization of gentamicin hydrogel infused with tetracarpidium conophorum extract via Central composite design for topical delivery

Ilomuanya

Amenaghawon

Odimegwu

et al. 2018

tjps

View full text Add to dashboard Cite

show abstract

“…Drug-loaded hydrogels were prepared according to previously demonstrated procedure [6, 16]. In general, there are four different classes of excipients usually incorporated in order to prepare topical gels which are gelling agents, humectants, preservatives, and vehicles [17].…”

Section: Methodsmentioning

confidence: 99%

“…Hydrogels have been investigated extensively for application as carriers in diffusion-controlled release devices [6]. Hydrogels are a common form of topical application.…”

Section: Introductionmentioning

confidence: 99%

Newly Developed Topical Cefotaxime Sodium Hydrogels: Antibacterial Activity andIn VivoEvaluation

Zakaria

Afifi

Elkhodairy

2016

BioMed Research International

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In an attempt to reach better treatment of skin infections, gel formulations containing Cefotaxime (CTX) were prepared. The gel was formulated using Carbopol 934 (C934), Hydroxypropyl Methylcellulose 4000 (HPMC 4000), Carboxymethylcellulose Sodium (Na CMC), Pectin (PEC), Xanthan Gum (XG), or Guar Gum (GG). Thirteen different formulas were prepared and characterized physically in terms of color, syneresis, spreadability, pH, drug content, and rheological properties. Drug-excipients compatibility studies were confirmed by FTIR and then in vitro drug release study was conducted. In vitro and in vivo antibacterial activities of CTX were studied against wound pathogens such as, Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and Pseudomonas aeruginosa (P. aeruginosa), using either pure drug or Fucidin® cream as control. F13 provides better spreadability compared to F1 (XG) or F11 (HPMC). Moreover, the release of the drug from hydrogel F13 containing C934 was slower and sustained for 8 h. Stability study revealed that, upon storage, there were no significant changes in pH, drug content, and viscosity of the gels. Also, F13 showed the larger inhibition zone and highest antibacterial activity among other formulations. Histological analysis demonstrated that after single treatment with F13 gel formulation, a noticeable reduction in microbial bioburden occurred in case of both Gram positive and Gram negative bacterial isolates.

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“…Topical and transdermal administration of ketamine have been investigated to avoid dizziness and nausea. Moreover, when the drug penetrates the skin, it acts directly on the small nerve fibres, achieving effective localised anaesthesia [8][9][10][11]. Transdermal ketamine has also been investigated as an anaesthetic for cosmetic and laser dermatology.…”

Section: Introductionmentioning

confidence: 99%

Preparation and assessment of ketamine hydrogels for prolonged transdermal anaesthesia

Liu¹,

Zheng²

2017

Trop. J. Pharm Res

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Evaluation of Hydrogels Based on Poloxamer 407 and Polyacrylic Acids for Enhanced Topical Activity of Gentamicin against Susceptible Infections

Cited by 16 publications

References 16 publications

Formulation and optimization of gentamicin hydrogel infused with tetracarpidium conophorum extract via Central composite design for topical delivery

Formulation and optimization of gentamicin hydrogel infused with tetracarpidium conophorum extract via Central composite design for topical delivery

Newly Developed Topical Cefotaxime Sodium Hydrogels: Antibacterial Activity andIn VivoEvaluation

Preparation and assessment of ketamine hydrogels for prolonged transdermal anaesthesia

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