Evaluation of hydrocarbons and organochlorine pesticides and their tolerant microorganisms from an agricultural soil to define its bioremediation feasibility

Abstract

The concentrations of hydrocarbons and organochlorine pesticides (OCPs), nutrients and tolerant microorganisms in an agricultural soil from a locality in Tepeaca, Puebla, Mexico, were determined to define its feasibility for bioremediation. The OCPs detected were heptachlor, aldrin, trans-chlordane, endosulfán I, endosulfán II, 1,1,1-bis-(4-chlorophenyl)-2,2-trichloroethane (4,4'-DDT), 1,1-bis-(4-chlorophenyl)-2,2-dichloroethene (4,4'-DDE) and endrin aldehyde, with values of 0.69-30.81 ng g(-1). The concentration of hydrocarbons in the soil of Middle Hydrocarbons Fraction (MHF), C10 to C28, was 4608-27,748 mg kg(-1) and 1117-19,610 mg kg(-1) for Heavy Hydrocarbons Fraction (HHF), C28 to C35, due to an oil spill from the rupture of a pipeline. The soil was deficient in nitrogen (0.03-0.07%) and phosphorus (0 ppm), and therefore it was advisable to fertilize to bio-stimulate the native microorganisms of soil. In the soil samples, hydrocarbonoclast fungi 3.72 × 10(2) to 44.6 × 10(2) CFU g(-1) d.s. and hydrocarbonoclast bacteria (0.17 × 10(5) to 8.60 × 10(5) CFU g(-1) d.s.) were detected, with a tolerance of 30,000 mg kg(-1) of diesel. Moreover, pesticideclast fungi (5.13 × 10(2) to 42.2 × 10(2) CFU g(-1) d.s.) and pesticideclast bacteria (0.15 × 10(5) to 9.68 × 10(5) CFU g(-1) d.s.) were determined with tolerance to 20 mg kg(-1) of OCPs. Fungi and bacteria tolerant to both pollutants were also quantified. Therefore, native microorganisms had potential to be stimulated to degrade hydrocarbons and pesticides or both pollutants. The concentration of pollutants and the microbial activity analyzed indicated that bioremediation of the soil contaminated with hydrocarbons and pesticides using bio-stimulation of native microorganisms was feasible.

Biodegradation of flubendiamide by a newly isolated Chryseobacterium sp. strain SSJ1

“…Bioremediation is a process of cleaning up of contaminated soils using biological means. This process ultimately aims towards forcing these native microbial populations to use contaminant (pesticide) as a source of carbon and nutrient for their growth and development (Islas-garcia et al 2015 ). Furthermore, it is necessary to perform physicochemical characterisation of contaminated soil in order to find out whether the native microbial species is capable of degrading the pesticide by biostimulation.…”

Agrotóxicos no Brasil: uma compreensão do cenário atual de utilização e das propriedades do solo que atuam na dinâmica e retenção destas moléculas

“…A eficiência microbiana da degradação é uma função da capacidade dos microrganismos degradadores de permanecerem ativos no ambiente. Islas-García et al (2015) estudaram diferentes parâmetros como a concentração de hidrocarbonetos e de pesticidas organoclorados, além de nutrientes e microrganismos tolerantes em um solo agrícola para definir sua viabilidade para biorremediação. A diminuição da concentração do poluente e o aumento da atividade microbiana indicaram que o uso da bioestimulação de microrganismos nativos era viável em um solo contaminado com hidrocarbonetos e agrotóxicos.…”

Biological Enhancement Based on Cast Process and Denitrification Performance of Solid Carbon Source for Low C/N Domestic Sewage

“…When the system needs additional carbon source for a long time, in order to avoid secondary pollution caused by high COD in the initial stage of solid carbon source immersion, the corn cob should be immersed for two days before drying and then using. When the system needs to add carbon source for short-term emergency treatment, corn cob soaking solution can be used instead of methanol which is currently used more (Huang et al, 2013;Islas-García et al, 2015).…”