Evaluation of hydro-alcoholic extract of Eclipta alba for its anticancer potential: An in vitro study

Chaudhary, Harshita; Dhuna, Vikram; Singh, Jatinder; Kamboj, Sukhdev Singh; Seshadri, Sriram

doi:10.1016/j.jep.2011.04.066

Cited by 54 publications

(36 citation statements)

References 22 publications

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“…The EH extract had higher level of anticancer potential compared to the MH extract, which could be because of the presence of higher phenolic and flavonoid content. Our results are in agreement with earlier work that the EH had anticancer capacity (Chaudhary et al, ), and the EH might be a valuable natural source in cancer therapy.…”

Section: Resultssupporting

confidence: 93%

“…Some phenolic compounds including p ‐coumaric acid, quercetin, luteolin, and apigenin were reported by Samson (Samson et al, ). This herb shows versatile pharmacological effects that include hair growth, antioxidant, antimicrobial, anti‐inflammatory and antihepatotoxic (Chauhan, Singh, & Painuli, ; Jadhav, Thorat, Kadam, & Salaskar, ), hepatoprotective activity (Ma‐Ma, Nyunt, & Tin, ), and anticancer activities (Chaudhary, Jena, Singh, & Seshadri, ). Antioxidant effect of the EH might be one of the underlying mechanisms for these bioactive effects.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant activities and HepG2 cells growth inhibitory capacity of whole plant ethanol extracts (Eclipta alba Hassk and Mesona procumbens Hemsl)

Lay

2017

J Food Biochem

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This study investigated in vitro antioxidant and hepatocellular carcinoma (HepG2) cells growth inhibitory effect of Eclipta alba Hassk (EH) and Mesona procumbens Hemsl (MH). Extracts from ethanol 50% (E50) were chosen for the assays. Total phenolic and flavonoid content and the antioxidant activities including ABTS 1 and DPPH radical-scavenging system were determined. The identification of phenolic compounds was analyzed by high performance liquid chromatography (HPLC). It was found that higher levels of DPPH and ABTS 1 radical-scavenging activity were detected from the EH extract with IC 50 of 41.91 6 0.08 and 75.49 6 1.15 mg/mL, respectively. The rate of DPPH radical-scavenging activity was significantly influenced by the reaction time. The 4hydroxybenzoic acid, caffeic acid, p-coumaric acid, and rosmarinic acid were the identified compounds in the EH. The 7-hydroxycoumarin, ferulic acid, and rutin being new compounds and two known compounds were identified in the MH. Lactate dehydrogenase released in medium was also evaluated. Extracts of the EH and MH stimulated the apoptotic factors bcl-2, bax, caspase-3, caspase-9 in HepG2. Overall, the EH extract was higher in total phenolic and flavonoid content, antioxidant potential and more effective in inhibiting cancer cell growth.

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Section: Resultssupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Antioxidant activities and HepG2 cells growth inhibitory capacity of whole plant ethanol extracts (Eclipta alba Hassk and Mesona procumbens Hemsl)

Lay

2017

J Food Biochem

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“…21 Recent studies have also highlighted the anticancer potential and anti-multidrug resistance properties of hydroalcoholic extract of the seeds of E alba. 22,23 Similarly, anti-inflammatory, antitumor-promoting activities of the crude extract and the constituents of C officinalis have been reported by two independent studies. 24,25 These extracts were standardized for their chemical constitution for their potential chemopreventive efficacy against skin carcinogenesis induced by DMBA and croton oil in mice.…”

Section: Introductionmentioning

confidence: 64%

Assessment of Augmented Immune Surveillance and Tumor Cell Death by Cytoplasmic Stabilization of p53 as a Chemopreventive Strategy of 3 Promising Medicinal Herbs in Murine 2-Stage Skin Carcinogenesis

Ali

Khan

et al. 2013

Integr Cancer Ther

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Cancer is the final outcome of a plethora of events. Targeting the proliferation or inducing programmed cell death in a proliferating population is a major standpoint in the cancer therapy. However, proliferation is regulated by several cellular and immunologic processes. This study reports the inhibition of proliferation by augmenting immune surveillance, silencing acute inflammation, and inducing p53-mediated apoptosis of skin cancer by 3 promising medicinal extracts. We used the well-characterized model for experimental skin carcinogenesis in mice for 32 weeks to study the chemopreventive effect of the methanolic extracts of Trigonella foenumgraecum, Eclipta alba, and Calendula officinalis. All 3 extracts reduced the number, incidence, and multiplicity of tumors, which was confirmed by the pathologic studies that showed regressed tumors. There was a significant reduction in the PCNA+ nuclei in all treatment groups 32 weeks after the initiation. Mechanistic studies revealed that proliferative population in tumors is diminished by the restoration of the endogenous antioxidant defense, inhibition of the stress-related signal-transducing element NFκB, reduction of inflammation, enhancement of immunosurveillance of the genetically mutated cells, along with silencing of the cell cycle progression signals. Finally, all 3 medicinal extracts induced stable expression of p53 within the tumors, confirmed by the CFDA-Cy3 apoptosis assay. Results of our study confirm that these extracts not only limit the rate of proliferation by inhibition of the processes integral to cancer development but also induce stable cytoplasmic expression of p53-mediated apoptosis, leading to fewer and regressed tumors in mice.

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“…It is used in the treatment of spleen enlargements, uterine hemorrhages, skin diseases, scorpion bites and respiratory disorders (Pithayanukul et al 2004;Khare, 2007). This plant has been experimentally proven to possesses nephroprotective, cerebroprotective, hepato-protective, anti-cancer, anti-asthma, mosquito larvicidal and ovicidal properties, toxicity against hepatitis-C virus, hair growth promoting activity, immunemodulatory property, antioxidant, lipid lowering activity and anti-venom property (Dungca, 2016;Chaudhary et al 2011;de Freitas Morel et al 2017;Jayathirtha and Mishra, 2004;Datta et al 2009;Govindarajan and Karuppannan, 2011). Presence of wedelolactone, ecliptamines, taraxastane glycosides, polythienyl and other phytochemicals has been reported in this plant (Zhang et al 2010;Liu et al 2012;Han et al 2013).…”

Section: Introductionmentioning

confidence: 94%

Development of Chemical Fingerprints to Differentiate the Controversial Indian Herbal Drugs Eclipta Prostrata and Wedelia Calendulacea (Bhringraja)

Vadivel¹,

Anand²,

Monajkumar³

et al. 2017

Bull. Pharm. Res.

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In Indian system of medicine, Eclipta prostrata is an important herbal ingredient of several Ayurvedic and Siddha formulations and it is commonly called as Bhringraja. But, this plant is often confused with Wedelia calendulacea, which is also known as Bhringraja and also being adulterated in Indian herbal market. In order to differentiate these plant drugs (Eclipta prostrata and Wedelia calendulacea), in the present work we have developed chemical fingerprints. Phytochemical analysis, UV-Visible, FT-IR and NMR spectroscopy, HPTLC and HPLC chromatography were carried out in the selected plant materials. Phytochemical analysis of methanolic extract of investigated materials showed that W. calendulacea contained higher level of total phenolic compounds (381.82 mg GAE/100 g) when compared to E. prostrata (105 mg GAE/100 g). Scanning in UV-Visible spectroscopy exhibited difference between selected samples, especially at 250-450 nm. Similarly, FT-IR spectroscopy of raw materials indicated notable difference between the samples at 400 -1000 cm -1 region. NMR spectroscopy illustrated remarkable difference between the chloroform extract of selected samples at 1-2 ppm region. HPTLC profile of methanolic extract of E. prostrata exhibited a total number of 9 peaks while W. calendulacea showed 12 number of peaks. Chemical profile investigated in the present study would be helpful to differentiate E. prostrata from W. calendulacea and also useful to prevent their adulteration in herbal industry.

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Evaluation of hydro-alcoholic extract of Eclipta alba for its anticancer potential: An in vitro study

Cited by 54 publications

References 22 publications

Antioxidant activities and HepG2 cells growth inhibitory capacity of whole plant ethanol extracts (Eclipta alba Hassk and Mesona procumbens Hemsl)

Antioxidant activities and HepG2 cells growth inhibitory capacity of whole plant ethanol extracts (Eclipta alba Hassk and Mesona procumbens Hemsl)

Assessment of Augmented Immune Surveillance and Tumor Cell Death by Cytoplasmic Stabilization of p53 as a Chemopreventive Strategy of 3 Promising Medicinal Herbs in Murine 2-Stage Skin Carcinogenesis

Development of Chemical Fingerprints to Differentiate the Controversial Indian Herbal Drugs Eclipta Prostrata and Wedelia Calendulacea (Bhringraja)

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