Evaluation of hyaluronic acid-P40 conjugated cream in a mouse model of dermatitis induced by oxazolone

Abstract

P40 is a particulate fraction or fragment isolated from Corynebacterium granulosum, which exhibits a wide spectrum of pharmacological functions including antitumor, antibacterial, phagocytic, antiviral and cytokine induction effects. In the present study, the immunomodulatory potential of P40-conjugated with hyaluronic acid was assessed in a mouse model of dermatitis induced by oxazolone. Oxazolone-induced allergic contact dermatitis is a T cell-mediated Th2-like hypersensitivity reaction, which mimics the corresponding reaction in humans. Female cluster of differentiation-1 mice were sensitized on days 0 and 1 by the application of 2% oxazolone onto a shaved back. The disease was induced by re-challenge on day 7 using 15% oxazolone in the inner and outer of the left ears of the mice. Mice were topically treated with hyaluronic acid-P40 conjugate cream or with placebo to the inner and outer surface of the left ear for 7 consecutive days starting from 1 h after the sensitization. A significant reduction in ear thickness and weight and in edema and leukocyte recruitment were observed in the mice treated with hyaluronic-P40 conjugate cream compared with mice treated with the cream base alone (P<0.05). Thus, P40-conjugated with hyaluronic acid may constitute an innovative dermatitis treatment.

Hyaluronic Acid in the Third Millennium

“…For instance, a variety of anti-inflammatory drugs, including hydrocortisone, prednisone, prednisolone and dexamethasone, have been conjugated to HA and investigated for intraarticular therapy of arthritis [166]. Furthermore, a recent study has displayed the potential of an emulsion containing a novel HA-P40 conjugate to treat a mouse model of dermatitis induced by oxazolone [180]. P40 is a particulate fragment isolated from Corynebacterium granulosum (actually known as Propionibacterium acnes ), which has immunomodulatory, antibacterial, antiviral and antitumor properties.…”

“…P40 is a particulate fragment isolated from Corynebacterium granulosum (actually known as Propionibacterium acnes ), which has immunomodulatory, antibacterial, antiviral and antitumor properties. The conjugation of P40 to HA successfully prevents its systemic absorption and, therefore, improves its topical therapeutic effect [180]. Other research has shown that conjugation of curcumin with HA represents a strategy to enhance the water solubility and stability of curcumin [181].…”

Atopic Dermatitis as a Multifactorial Skin Disorder. Can the Analysis of Pathophysiological Targets Represent the Winning Therapeutic Strategy?

“…In a mouse model the topical application of p40, a particulate fraction from Corynebacterium granulosum , used in a formula with hyaluronic acid produced a significant reduction in ear thickness, weight, oedema, and leukocyte recruitment. These results suggest that p40-conjugated with hyaluronic acid may constitute an outstanding innovative dermatitis treatment [ 71 ].…”

Hyaluronic Acid in the 3^rd Millennium

“…P40 is a particulate fragment isolated from Corynebacterium granulosum (actually known as Propionibacterium acnes) which has immunomodulatory, antibacterial, antiviral and antitumor properties. The conjugation of P40 to HA successfully prevents its systemic absorption and, therefore, improves its topical therapeutic effect [180]. Other researches have shown that conjugation of curcumin with HA represents a strategy to enhance water solubility and stability of curcumin [181].…”

“…For instance, a variety of antinflammatory drugs, including hydrocortisone, prednisone, prednisolone and dexamethasone, have been conjugated to HA and investigated for intra-articular therapy of arthritis [166]. Furthermore, a recent study has displayed the potential of an emulsion containing a novel HA-P40 conjugate to treat a mouse model of dermatitis induced by oxazolone [180]. P40 is a particulate fragment isolated from Corynebacterium granulosum (actually known as Propionibacterium acnes) which has immunomodulatory, antibacterial, antiviral and antitumor properties.…”