Evaluation of human mesenchymal stem cells response to biomimetic bioglass‐collagen‐hyaluronic acid‐phosphatidylserine composite scaffolds for bone tissue engineering

Xu, Caixia; Wang, Yingjun; Yu, Xiaoli; Chen, Xiaofeng; Li, Xiangjun; Yang, Xuhui; Shu-nong, LI; Zhang, Xiuming; Xiang, Andy Peng

doi:10.1002/jbm.a.31931

Cited by 31 publications

(15 citation statements)

References 31 publications

(58 reference statements)

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“…To enhance the properties of hyaluronan, it can be crosslinked with gelatin and implanted in situ into a defect, showing superior integration of the repaired tissue (Liu et al ., ). In addition, bioglass–collagen–HY–phosphatidylserine (PS) (BCHP) composite porous scaffolds exhibited initial attachment, proliferation, migration and differentiation of the cells on the scaffold (Xu et al ., , ): once implanted, this composite scaffold showed a low inflammatory response and foreign body response within 3 weeks, together with a higher degree of healing, as visible from Figure .…”

Section: Natural Polymersmentioning

confidence: 81%

Polymeric scaffolds as stem cell carriers in bone repair

Rossi

Santoro

Perale

2013

J Tissue Eng Regen Med

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Although bone has a high potential to regenerate itself after damage and injury, the efficacious repair of large bone defects resulting from resection, trauma or non-union fractures still requires the implantation of bone grafts. Materials science, in conjunction with biotechnology, can satisfy these needs by developing artificial bones, synthetic substitutes and organ implants. In particular, recent advances in polymer science have provided several innovations, underlying the increasing importance of macromolecules in this field. To address the increasing need for improved bone substitutes, tissue engineering seeks to create synthetic, three-dimensional scaffolds made from polymeric materials, incorporating stem cells and growth factors, to induce new bone tissue formation. Polymeric materials have shown a great affinity for cell transplantation and differentiation and, moreover, their structure can be tuned in order to maintain an adequate mechanical resistance and contemporarily be fully bioresorbable. This review emphasizes recent progress in polymer science that allows relaible polymeric scaffolds to be synthesized for stem cell growth in bone regeneration.

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Section: Natural Polymersmentioning

confidence: 81%

Polymeric scaffolds as stem cell carriers in bone repair

Rossi

Santoro

Perale

2013

J Tissue Eng Regen Med

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“…Various osteogenic cell sources and biodegradable scaffolds, including synthetic and natural polymers [32, 52-55], ceramics [56] and composites [54, 57-59] have also been tested in static culture. These studies helped identify the importance of three-dimensional culture environments for proper signaling (cell condensation, cell-cell interactions and cell-matrix interactions) that can stimulate osteogenesis, but they also showed clearly that static culture limits the development of bone constructs due to the diffusional exchange of nutrients, oxygen and metabolites.…”

Section: Tissue-engineered Bone Graftsmentioning

confidence: 99%

Tissue Engineered Bone Grafts: Biological Requirements, Tissue Culture and Clinical Relevance

Fröhlich

Grayson

Wan

et al. 2008

CSCR

290

239

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The tremendous need for bone tissue in numerous clinical situations and the limited availability of suitable bone grafts are driving the development of tissue engineering approaches to bone repair. In order to engineer viable bone grafts, one needs to understand the mechanisms of native bone development and fracture healing, as these processes should ideally guide the selection of optimal conditions for tissue culture and implantation. Engineered bone grafts have been shown to have capacity for osteogenesis, osteoconduction, osteoinduction and osteointegration - functional connection between the host bone and the graft. Cells from various anatomical sources in conjunction with scaffolds and osteogenic factors have been shown to form bone tissue in vitro. The use of bioreactor systems to culture cells on scaffolds before implantation further improved the quality of the resulting bone grafts. Animal studies confirmed the capability of engineered grafts to form bone and integrate with the host tissues. However, the vascularization of bone remains one of the hurdles that need to be overcome if clinically sized, fully viable bone grafts are to be engineered and implanted. We discuss here the biological guidelines for tissue engineering of bone, the bioreactor cultivation of human mesenchymal stem cells on three-dimensional scaffolds, and the need for vascularization and functional integration of bone grafts following implantation.

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“…In addition, the response of MSCs on the BG‐COL‐HYA‐PS composite scaffold was assessed in comparison with those on pure 58sBG, both BG‐COL and BG‐COL‐HYA composites. The results demonstrated that MSCs grown on BG‐COL‐HYA‐PS composite porous scaffolds exhibited a higher degree of attachment, growth, migration speed, and osteogenic differentiation than those on the pure 58sBG, BG‐COL, and BG‐COL‐HYA scaffolds in vitro 16…”

Section: Introductionmentioning

confidence: 99%

A novel biomimetic composite scaffold hybridized with mesenchymal stem cells in repair of rat bone defects models

Wang

et al. 2010

J Biomedical Materials Res

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In this study, the in vivo bone-regenerative potential of a novel bioactive glass-collagen-hyaluronic acid-Phosphatidylserine (BG-COL-HYA-PS) composite scaffold hybridized with mesenchymal stem cells (MSCs) was investigated in a rat bone defect model. HrGFP-labeled MSCs were cultured for 2 weeks on the BG-COL-HYA-PS scaffold before implantation into the defect. A cell-free scaffold and an untreated defect were used as controls. The regeneration process was evaluated by histology, X-ray, and mechanical rigidity experiments at different time points post-implantation. The results revealed that BG-COL-HYA-PS scaffold exhibited a low inflammatory response and foreign body response within 3 weeks. At week 6, those responses disappeared following the resorption of scaffolds and the formation of new bone. Compared with the pure scaffold or empty group, the introduction of MSCs into the porous scaffold dramatically enhanced the efficiency of the new bone formation and biomechanical property of the femur. In addition, the transplanted MSCs could survive for up to 3 weeks or longer. The results demonstrated that the BG-COL-HYA-PS scaffold was biocompatible and osteoconductive and the transplanted MSCs with the scaffold enhanced the healing of the bone defect.

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Evaluation of human mesenchymal stem cells response to biomimetic bioglass‐collagen‐hyaluronic acid‐phosphatidylserine composite scaffolds for bone tissue engineering

Cited by 31 publications

References 31 publications

Polymeric scaffolds as stem cell carriers in bone repair

Polymeric scaffolds as stem cell carriers in bone repair

Tissue Engineered Bone Grafts: Biological Requirements, Tissue Culture and Clinical Relevance

A novel biomimetic composite scaffold hybridized with mesenchymal stem cells in repair of rat bone defects models

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