Evaluation of human hemoglobin vesicle as an oxygen carrier: recovery from hemorrhagic shock in rabbits

Ogata, Yoshitaka

doi:10.1002/1099-1581(200006)11:6<301::aid-pat981>3.0.co;2-d

Cited by 10 publications

(9 citation statements)

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“…Nonetheless, safety concerns and untoward effects still prevent clinical application, whereas short functional life may reduce their utility as blood substitutes. 6 -12 Considering its perfusion characteristics deriving from its size and viscosity, 11,12 we performed the current experiments to examine the hypothesis that LEH may better-perfuse collaterals and capillaries with plasma to reduce ischemic and reperfusion damage. The results suggest that LEH in a small dose (2 mL/kg) is effective in reducing the extent of infarction …”

Section: Discussionmentioning

confidence: 99%

“…11,12 Briefly, it is a liposome capsule measuring 230 nm in mean diameter, containing hemoglobin eluted from human RBC outdated for transfusion. The liposome capsule is coated with polyethylene glycol to reduce aggregation and capture by the reticuloendothelial system to prolong the circulation half-life to 30 hours in rats.…”

Section: Lehmentioning

confidence: 99%

“…Whereas LEH is considered to be more efficient in O 2 transport than RBC under the room air respiration, RBC is considered to be more efficient under hypoxic conditions ( Figure 1). LEH is suspended in saline to a hemoglobin concentration of 6 g/dL or 25% of volume (LEH-crit), which has a comparable O 2 carrying capacity ( Figure 1) and a reduced viscosity (2 cp) 12 compared with blood (5 cp). LEH is precipitated between plasma and RBC by a centrifuge with 50 000g for 120 minutes.…”

Section: Lehmentioning

confidence: 99%

“…Recently, cell-free hemoglobin 8 -10 has been developed as an artificial O 2 carrier; renal clearance and hypertensive response obscured neuroprotective effects in experiments 8,9 and in a human trial. 10 Liposomeencapsulated hemoglobin (LEH) 11,12 may be effective in acute brain ischemia, because its size (230 nm) may not only prevent extravasation but also allow O 2 delivery beyond the obstruction with plasma to areas where red blood cells (RBCs) seldom reach, thereby reducing the O 2 diffusion distance. Sigmoid O 2 dissociation characteristics may allow more efficient O 2 delivery in room air than perfluorocarbon, which needs a higher O 2 inspiration because of its linear O 2 dissociation capacity.…”

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confidence: 99%

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Liposome-Encapsulated Hemoglobin Reduces the Size of Cerebral Infarction in the Rat

Kawaguchi

Fukumoto

Haida

et al. 2007

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Background and Purpose-Liposome-encapsulated hemoglobin (LEH; TRM-645) is a novel oxygen (O 2 ) carrier with a lower O 2 affinity (P 50 O 2 ϭ40 mm Hg) than red blood cells. In contrast to cell-free hemoglobin, encapsulation prevents hemoglobin extravasation, whereas its subcellular size (230 nm) may improve O 2 delivery and limit the severity of cerebral infarction. Methods-The extent of cerebral infarction was determined 24 hours after photochemically induced thrombosis of the middle cerebral artery from the integrated area of infarction detected by triphenyltetrazolium chloride staining in rats receiving no treatment, 10 mL/kg of LEH, homologous blood, empty liposomes, or saline. To develop a dose-response relationship, LEH dose was reduced from 10 mL/kg to 2 mL/kg, 0.4 mL/kg, and 0.08 mL/kg. Results-Infarction extent was significantly suppressed in rats receiving LEH as compared with animals receiving no infusion, saline, empty liposome, or transfusion in the cortex but not in the basal ganglia, where all had similar degrees of damage. The dose-response relationship revealed that as little as 2 mL/kg of LEH was protective, whereas the total blood O 2 content, hemoglobin level, and transfusion and/or infusion of empty liposomes or saline were not effective. Conclusions-Our results suggest that LEH significantly reduces the area of infarction in the cortex but not in basal ganglia after photochemically induced thrombosis of the middle cerebral artery in the rat.

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Section: Discussionmentioning

confidence: 99%

Section: Lehmentioning

confidence: 99%

Section: Lehmentioning

confidence: 99%

mentioning

confidence: 99%

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Liposome-Encapsulated Hemoglobin Reduces the Size of Cerebral Infarction in the Rat

Kawaguchi

Fukumoto

Haida

et al. 2007

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“…20,21 Modificações estruturais da Hb incluem: ligação quí-mica cruzada a fim de estabilizar os tetrâmeros de Hb; polimerização da Hb produzindo moléculas de alto peso molecular, encapsulação de vários preparados de Hb e molé-culas de Hb recombinantes por engenharia genética. 22,23,24 Em 1957, Chang preparou Hb artificial encapsulada com o intuito de produzir glóbulos vermelhos artificiais. 25 O mesmo autor, em 1964, demonstrou que seria possível a produção de Hb ligada por pontes intermoleculares, estabilizando a Hb.…”

Section: Introductionunclassified

Importância dos carreadores de oxigênio livre de células

Novaretti¹

2007

Rev. Bras. Hematol. Hemoter.

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IntroduçãoNo mundo todo, a necessidade de transfusões sangüíneas vem crescendo progressivamente. No Brasil, enquanto a demanda de sangue cresce à taxa de 1% ao ano, as expectativas de crescimento das doações de sangue variam de 0,5 a 0,7%/ano. 1 Conseqüentemente, a estimativa para o ano de 2030 é de um déficit de aproximadamente um milhão de unidades de sangue coletado, levando-se em conta o crescimento populacional projetado e as necessidades crescentes de transfusão, em especial para os que a necessitam cronicamente, para cirurgias de grande complexidade e no atendimento às emergências.2 Deve-se ressaltar que essa projeção não inclui situações de calamidade pública e desastres naturais.Se estimarmos as necessidades internacionais de transfusão de sangue e componentes, tendo por base a estimativa de 0,05 unidades per capita por ano, pode ser projetada uma demanda de assustadoras 300 milhões de unidades de sangue por ano.3 Quando são computados os números disponí-veis de transfusões de sangue em termos mundiais, temos cerca de 90 milhões de unidades transfundidas por ano. Caso esse número seja verdadeiro, há falta de 200 milhões de bolsas de sangue/ano. 4 Obviamente essas estimativas estão sujeitas a críticas, mas é evidente que não há suprimento total de sangue, particularmente de alguns tipos (como os Rhnegativo) em situações emergenciais, e a sazonalidade é importante fator que compromete o fornecimento de sangue. Deve-se ressaltar que, apesar de os progressos para o aumento da segurança transfusional e da diminuição vertigi-

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Liposome-Encapsulated Hemoglobin as an Artificial Oxygen Carrier: Technological Features, Manufacturing and Issues for Practical Application

Kaneda

Ishizuka

Gotô

et al. 2013

Hemoglobin-Based Oxygen Carriers as Red Cell Substitutes and Oxygen Therapeutics

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Evaluation of human hemoglobin vesicle as an oxygen carrier: recovery from hemorrhagic shock in rabbits

Cited by 10 publications

References 10 publications

Liposome-Encapsulated Hemoglobin Reduces the Size of Cerebral Infarction in the Rat

Liposome-Encapsulated Hemoglobin Reduces the Size of Cerebral Infarction in the Rat

Importância dos carreadores de oxigênio livre de células

Liposome-Encapsulated Hemoglobin as an Artificial Oxygen Carrier: Technological Features, Manufacturing and Issues for Practical Application

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