Evaluation of housekeeping genes for normalizing RT-qPCR and analysis of the expression patterns of WRKY1 transcription factor and rhynchophylline biosynthetic-related genes in Uncaria rhynchophylla
Abstract:Background: Uncaria rhynchophylla(Miq.)Miq.ex Havil, a traditional medicinal herb, is enriched with a number of pharmacological active terpenoid indole alkaloids (TIAs). At present, a comprehensive selection and evaluation of the appropriate housekeeping genes for gene expression analysis, especially transcription factors and key enzyme genes involved in biosynthesis pathway of TIAs in U. rhynchophylla, have not been reported. Reverse transcription quantitative PCR (RT-qPCR) is currently the most common method… Show more
Set email alert for when this publication receives citations?
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.