Evaluation of honey as an antibacterial agent against drug-resistant uropathogenic <i>E. coli</i> strains

Mohamed, Sara H.; Elshahed, Maram M. S.; Saied, Yasmine M.

doi:10.5958/0974-360x.2020.00658.7

Cited by 5 publications

(3 citation statements)

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“…Our study further found that honey exhibited high antibacterial activity against E. coli, which aligns with previous findings demonstrating honey's effectiveness against E. coli [59,[98][99][100] . The antibacterial activity of honey could be due to the presence of hydrogen peroxide which is a disinfectant and a strong oxidizing agent [39,55] .…”

Section: Discussionsupporting

confidence: 92%

“…In Scotland, honey was reported to have antibacterial activity against E. coli due to its phytochemical composition such as the novel fatty diacid glycoside derivatives [77] . A study in Egypt revealed that honey was effective against antibiotic-resistant E. coli [100] , similar to reports from Ethiopia [94] . This makes honey a valuable potential alternative or source of antibiotics after further investigations.…”

Section: Discussionsupporting

confidence: 73%

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Antibacterial activities of honey against Escherichia coli and Staphylococcus aureus: A potential treatment for bacterial infections and alternative to antibiotics

Mudenda¹,

Hikaambo²,

Chabalenge³

et al. 2023

J Pharmacogn Phytochem

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The global problem of antimicrobial resistance (AMR) has increased interest in natural products, such as honey in the treatment of diseases. This study evaluated the antibacterial activities of honey against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). This cross-sectional study was conducted from November 2020 to May 2021 using E. coli and S. aureus as indicator organisms. The antibacterial activities of honey were assessed using the agar well diffusion assay. The sensitivities of E. coli and S. aureus to honey were indicated by zones of inhibition which were measured using a ruler in millimetres. Data analysis was performed using Stata version 16.1. Honey produced a dose-dependent antibacterial activity against E. coli and S. aureus. Our study demonstrated that honey had stronger antibacterial activities against E. coli compared to S. aureus. This study highlights the need for further research on honey to investigate its potential use in treating bacterial infections.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 73%

Antibacterial activities of honey against Escherichia coli and Staphylococcus aureus: A potential treatment for bacterial infections and alternative to antibiotics

Mudenda¹,

Hikaambo²,

Chabalenge³

et al. 2023

J Pharmacogn Phytochem

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“…Recently, two series of synthetic chemical compounds ( 1a – e and 2a – e ) were tested against different highly virulent strains of clinical isolates E.coli , K. pneumonia , and Staphylococcus aureus MRSA that previously showed resistance to ciprofloxacin [ 25 , 27 , 28 , 29 ], besides two reference strains ( Staphylococcus aureus ATCC 25923, and E. coli ATCC 11229) and exhibited good antibacterial activity [ 26 ] as depicted in Figure 2 and Table S1 (cf. supplementary file) .…”

Section: Resultsmentioning

confidence: 99%

Experimental and Molecular Docking Studies of Cyclic Diphenyl Phosphonates as DNA Gyrase Inhibitors for Fluoroquinolone-Resistant Pathogens

et al. 2022

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DNA gyrase and topoisomerase IV are proven to be validated targets in the design of novel antibacterial drugs. In this study, we report the antibacterial evaluation and molecular docking studies of previously synthesized two series of cyclic diphenylphosphonates (1a–e and 2a–e) as DNA gyrase inhibitors. The synthesized compounds were screened for their activity (antibacterial and DNA gyrase inhibition) against ciprofloxacin-resistant E.coli and Klebsiella pneumoniae clinical isolates having mutations (deletion and substitution) in QRDR region of DNA gyrase. The target compound (2a) that exhibited the most potent activity against ciprofloxacin Gram-negative clinical isolates was selected to screen its inhibitory activity against DNA gyrase displayed IC50 of 12.03 µM. In addition, a docking study was performed with inhibitor (2a), to illustrate its binding mode in the active site of DNA gyrase and the results were compatible with the observed inhibitory potency. Furthermore, the docking study revealed that the binding of inhibitor (2a) to DNA gyrase is mediated and modulated by divalent Mg2+ at good binding energy (–9.08 Kcal/mol). Moreover, structure-activity relationships (SARs) demonstrated that the combination of hydrazinyl moiety in conjunction with the cyclic diphenylphosphonate based scaffold resulted in an optimized molecule that inhibited the bacterial DNA gyrase by its detectable effect in vitro on gyrase-catalyzed DNA supercoiling activity.

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Detection of Heavy Metal Tolerance among different MLSB Resistance Phenotypes of Methicillin-Resistant S. aureus (MRSA)

Mohamed¹,

Elshahed²,

Saied³

et al. 2020

J Pure Appl Microbiol

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Methicillin-resistant Staphylococcus aureus (MRSA) strains are widespread globally. Besides their virulence factors, the co-occurrence of antimicrobial and metal resistance has been reported. This study was designed to evaluate the antibiotic resistance and resistance phenotypes, investigate the occurrence of virulence factors, and detect heavy metal tolerance among MRSA strains. Antibiogram profiling was done as recommended by CLSI instructions. Resistance phenotypes were detected by D test, followed by characterization of enzymatic activities and biofilm formation assay. Antibacterial activity of different heavy metals was tested, and predictable synergistic assay was performed. Among MRSA strains collected, high resistance to ampicillin and amoxicillin/clavulanate (100%) and high susceptibility to clindamycin (70%) were obtained. Resistance phenotypes were detected as S, constitutive MLSB, inducible MLSB, and MS by percentages of 10%, 30%, 30% and 30% respectively. Virulence factors like lipolytic (50%) and hemolytic (70%) activity, and biofilm formation ability (100%) were detected. High resistance towards potassium and magnesium was observed. MTC of 500 ppm was detected for all isolates in case of cobalt and iron. In case of zinc and copper, MTC was detected as 500 ppm except for one isolate which was highly resistant, and 500 ppm for all isolates except for two isolates which were highly sensitive respectively. Magnesium in different concentrations (500 and 2000 ppm) showed synergistic activity with erythromycin and clindamycin. Results reveal high heavy metal tolerance among antibiotic resistant MRSA strains, in addition to the presence of virulence factors. Upcoming studies must be focused on the combination of sub-inhibitory concentration of different heavy metals with the available antibiotics.

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Evaluation of honey as an antibacterial agent against drug-resistant uropathogenic E. coli strains

Cited by 5 publications

References 0 publications

Antibacterial activities of honey against Escherichia coli and Staphylococcus aureus: A potential treatment for bacterial infections and alternative to antibiotics

Antibacterial activities of honey against Escherichia coli and Staphylococcus aureus: A potential treatment for bacterial infections and alternative to antibiotics

Experimental and Molecular Docking Studies of Cyclic Diphenyl Phosphonates as DNA Gyrase Inhibitors for Fluoroquinolone-Resistant Pathogens

Detection of Heavy Metal Tolerance among different MLSB Resistance Phenotypes of Methicillin-Resistant S. aureus (MRSA)

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