Evaluation of histidine-containing dipeptides in twelve marine organisms and four land animal meats by hydrophilic interaction liquid chromatography with ultraviolet detection

Mori, Akira; Hikihara, Risako; Ishimaru, Mami; Hatate, Hideo; Tanaka, Ryusuke

doi:10.1080/10826076.2018.1526803

Cited by 9 publications

(6 citation statements)

References 28 publications

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“…The ANS content of ordinary muscle in skipjack tuna was higher than that in dark muscle. IDPs quantification results of the above five samples were consistent with previously reported data [11,[14][15][16]. Therefore, although the sample matrix effect needs to be considered individually, this method can be used to quantify the IDPs in various biological samples.…”

Section: Application To Other Biological Samplessupporting

confidence: 90%

“…The LOQs of ANS, CAR, and BAL were also evaluated as 0.11 µmol/L, 0.21 µmol/L, and 0.12 µmol/L, respectively (Table 2). These LOQs (ANS, 26 ng/mL; CAR, 48 ng/mL; BAL, 30 ng/mL) were lower than those previously reported for the HILIC-HPLC method [15,16]. The quantification values of IDPs in all dolphin sera analyzed in this study exceeded these LOQ values.…”

Section: Calibration Curves Lod and Loqcontrasting

confidence: 66%

“…HPLC, followed by ultraviolet (UV) detection, is often used for the analytical separation and detection of IDPs in foods and living organisms. For simple, non-derivative HPLC analysis of IDPs, the hydrophilic interaction chromatography (HILIC) mode [15][16][17] and reversed-phase (RP) mode [18][19][20][21] have both been applied. The HILIC mode is generally inferior in terms of separation and also more difficult to handle than the RP mode.…”

Section: Introductionmentioning

confidence: 99%

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Quantification of Histidine-Containing Dipeptides in Dolphin Serum Using a Reversed-Phase Ion-Pair High-Performance Liquid Chromatography Method

et al. 2021

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The quantification of histidine-containing dipeptides (anserine, carnosine, and balenine) in serum might be a diagnostic tool to assess the health condition of animals. In this study, an existing reversed-phase ion-pair high-performance liquid chromatography (HPLC)–ultraviolet detection method was improved and validated to quantify serum anserine, carnosine, and balenine levels in the dolphin. The serum was deproteinized with trichloroacetic acid and directly injected into the HPLC system. Chromatographic separation of the three histidine-containing dipeptides was achieved on a TSK–gel ODS-80Ts (4.6 mm × 150 mm, 5 µm) analytical column using a mobile phase of 50 mmol/L potassium dihydrogen phosphate (pH 3.4) containing 6 mmol/L 1-heptanesulfonic acid and acetonitrile (96:4). The standard curve ranged from 0.1 µmol/L to 250 µmol/L. The average accuracy of the intra- and inter-analysis of anserine, carnosine, and balenine was 97–106%. The relative standard deviations of total precision (RSDr) of anserine, carnosine, and balenine in dolphin serum were 5.9%, 4.1%, and 2.6%, respectively. The lower limit of quantification of these compounds was 0.11–0.21 µmol/L. These results indicate that the improved method is reliable and concise for the simultaneous determination of anserine, carnosine, and balenine in dolphin serum, and may be useful for evaluation of health conditions in dolphins. Furthermore, this method can also be applied to other biological samples.

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Section: Application To Other Biological Samplessupporting

confidence: 90%

Section: Calibration Curves Lod and Loqcontrasting

confidence: 66%

Section: Introductionmentioning

confidence: 99%

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Quantification of Histidine-Containing Dipeptides in Dolphin Serum Using a Reversed-Phase Ion-Pair High-Performance Liquid Chromatography Method

et al. 2021

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“…Interestingly, these MMPs also showed antioxidant action that was enhanced as pH was increased to alkaline conditions, which could, at least in part, reflect the presence of endogenous antioxidant peptides that have adapted to the alkalinity of marine conditions 241 . Antioxidants play an important role in protecting marine organisms from oxidative stress 245,246 and endogenous antioxidant peptides, such as glutathione, carnosine, anserine and balenine, have been identified in a variety of these organisms, including fish, seals, whales and dolphins [247][248][249] . In addition, uncharacterized endogenous antioxidant peptides have been reported in Ocypoda macrocera (The red ghost crab) 250 , Actinopyga lecanora (The stone fish or sea cucumber) 251 , Galatea paradoxa (The common galatea clam) and Patella rustica (The rustic limpet) 252 .…”

Section: Amps From Marine Sourcesmentioning

confidence: 99%

Antimicrobial Peptides with pH-Dependent Activity and Alkaline Optima: Their Origins, Mechanisms of Action and Potential Applications

Phoenix

Harris

Dennison

2021

CPPS

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: A number of disorders and diseases are associated with conditions of high pH and many conventional antibiotics lose their efficacy under these pH conditions, generating a need for novel antimicrobials, and a potential solution to fulfil this need is antimicrobial peptides (AMPs) with high pH optima. This review shows that a variety of anionic and cationic AMPs with this pH dependency are produced by creatures across the eukaryotic kingdom, including humans, rabbits, cattle, sheep, fish and frogs. These AMPs exhibit activity against viruses, bacteria and fungi that involves membrane interactions and appear to be facilitated by a variety of mechanisms that generally promote passage across membranes to attack intracellular targets, such as DNA or protein synthesis, and / or membrane lysis. Some of these mechanisms are unknown but those elucidated include the use of bacterial pores and transporters, the self-promoted uptake pathway and established models of membrane interaction, such as the carpet mechanism, toroidal pore formation, the adoption of tilted peptide and the SHM model. A variety of potential roles have been proposed for these AMPs, including use as antivirals, antibacterials, antifungals, adjuvants to antimicrobial therapy, biomarkers of disease and probes for pathogenic microbes. In this review, these properties are described and discussed, with an emphasis on the antimicrobial mechanisms used by these AMPs and the pH dependency of these mechanisms.

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“…IDPs were analyzed using an HPLC method that we developed previously (Mori et al 2018). The HPLC system included a PU-980 pump, a DG-980-50 degasser, a CO-965 column oven (40 °C), a UV-970 UV-VIS detector (wavelength 214 nm) (JASCO Corporation, Tokyo, Japan), and a 7725i Rheodyne-sampling injector that was connected to a 20-μL sample loop (Rheodyne, Rohnert Park, CA, USA).…”

Section: Hplc Systemmentioning

confidence: 99%

Ability of Three Kind of Imidazole Dipeptides, Carnosine, Anserine, and Balenine, to Interact with Unsaturated Fatty Acid-Derived Aldehydes and Carbohydrate-Derived Aldehydes

Mori

Hatate

Tanaka

2019

Int J Pept Res Ther

Self Cite

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Imidazole dipeptides (IDPs) such as carnosine (CAR), anserine (ANS), and balenine (BAL) are widely distributed in the skeletal muscle of vertebrates. Recently, several studies have revealed that CAR plays an important role in the detoxification of cytotoxic aldehydes arising from the peroxide of unsaturated fatty acids and carbohydrate metabolite. Although intensive studies on the detoxification of aldehydes by CAR have been performed, few studies have focused on the effects of detoxification by ANS and BAL. To determine the potential of minor IDPs such as ANS and BAL to react with cytotoxic aldehydes, the present study was established to investigate the consumption of IDP after co-incubation with cytotoxic aldehydes using high-performance liquid chromatography (HPLC). In the case of unsaturated fatty acid-derived aldehydes such as 4-hydroxy-2-trans-nonenal (from n-6 fatty acid) and 4-hydroxy-2-trans-hexenal (from n-3 fatty acid), ANS and CAR decreased considerably after co-incubation, but BAL did not. In the case of 3-deoxyglucosone and methylglyoxal as carbohydrate metabolites, no IDPs decreased after co-incubation; however, the absorbance at 336 nm of the CAR and BAL mixtures increased dramatically in a time-dependent manner. In the case of glyceraldehyde, which is also a carbohydrate metabolite, all IDPs, especially BAL, decreased after co-incubation and a new peak, surmised to represent an IDP-glyceraldehyde adduct, appeared on the HPLC chromatogram. These results can help explain the unique function and behavior of ANS and BAL in specific species.

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Evaluation of histidine-containing dipeptides in twelve marine organisms and four land animal meats by hydrophilic interaction liquid chromatography with ultraviolet detection

Cited by 9 publications

References 28 publications

Quantification of Histidine-Containing Dipeptides in Dolphin Serum Using a Reversed-Phase Ion-Pair High-Performance Liquid Chromatography Method

Quantification of Histidine-Containing Dipeptides in Dolphin Serum Using a Reversed-Phase Ion-Pair High-Performance Liquid Chromatography Method

Antimicrobial Peptides with pH-Dependent Activity and Alkaline Optima: Their Origins, Mechanisms of Action and Potential Applications

Ability of Three Kind of Imidazole Dipeptides, Carnosine, Anserine, and Balenine, to Interact with Unsaturated Fatty Acid-Derived Aldehydes and Carbohydrate-Derived Aldehydes

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