Evaluation of hGM-CSF/hTNFα surface-modified prostate cancer therapeutic vaccine in the huPBL-SCID chimeric mouse model

Lai, Shouhua; Huang, Zhiyong; Guo, Yunting; Cui, Yunqin; Wang, Lei; Ren, Weifeng; Ying, Furong; Gao, Hui; He, Lingxia; Zhou, Tong; Jiang, Jie‐Gen; Gao, Jimin

doi:10.1186/s13045-015-0175-8

Cited by 1 publication

(2 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The changes in gene expression in the cells were mainly related to cell metabolism, but the cell morphology and the original immunogenicity of its own didn’t change significantly [ 41 ]. Indeed, we didn’t notice any significant changes in terms of PC-3 immunogenicity, since we attempted to prepare large-scale culture of PC3 by use of our optimized media for the generation of hGM-CSF/hTNFα-surface-modified PC3 therapeutic vaccine for the prostate cancer [ 5 ].…”

Section: Discussionmentioning

confidence: 99%

“…The first therapeutic cancer vaccine of prostate cancer approved by FDA is Sipuleucel-T , which is essentially an autologous ex-vivo dendritic cell-based vaccine, pulsed with a single antigen of prostatic acid phosphatase (PAP) fused to GM-CSF [ 3 , 4 ]. Our novel therapeutic prostate cancer vaccine has been developed by use of GM-CSF/TNFα surface-modified PC-3 cells to treat the patients with advanced prostate cancer [ 5 ], and thus large-scale production of anchorage-dependent PC-3 prostate cancer cells will be required for vaccine manufacturing.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Use of real-time cellular analysis and Plackett-Burman design to develop the serum-free media for PC-3 prostate cancer cells

et al. 2017

Self Cite

View full text Add to dashboard Cite

In this study, we developed a rapid strategy to screen a serum-free medium for culturing the anchorage-dependent PC-3 prostate cancer cells, which was going to be prepared in large scale to generate GM-CSF/TNFα-surface-modified whole cell prostate cancer vaccine. Automated real-time cellular analysis as a rapid and non-invasive technology was used to monitor the growth of PC-3 cells in 16-well plates. At the same time, Plackett-Burman design was employed to identify the most influential formulation by integrating relevant information statistically. The effects of the 16 selected factors were evaluated during exponential cell growth and three medium constituents (EGF, FGF and linoleic acid) were identified to have significant effects on the cell growth. Subsequently, the response surface methodology with central composite design was applied to determine the interactions among the three factors so that these factors were optimized to improve cell growth. Finally, the prediction of the best combination was made under the maximal response to optimize cell growth by Design-Expert software 7.0. A total of 20 experiments were conducted to construct a quadratic model and a second-order polynomial equation. With the optimized combination validated by the stability test of serial passaging PC-3 cells, the serum-free medium had similar cell density and cell viability to the original serum medium. In summary, this high-throughput scheme minimized the screening time and may thus provide a new platform to efficiently develop the serum-free media for adherent cells.

show abstract

Section: Discussionmentioning

confidence: 99%