Evaluation of hepatotoxicity and oxidative stress in rats treated with tert-butyl hydroperoxide

Oh, Jung Min; Jung, Young Suk; Jeon, Byung-Suk; Yoon, Byung Il; Lee, Kye Sook; Kim, Bong Hee; Oh, Soo Jin; Kim, Sang Kyum

doi:10.1016/j.fct.2012.01.031

Cited by 26 publications

(25 citation statements)

References 38 publications

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“…Results from this study confirmed t -BHP-induced hepatotoxic effects as shown by the significant increase in the activity of ALT, AST and LDH in the serum of t -BHP-treated rats. These observations are in accordance with those obtained by previous studies [66, 87–89]. Alanine amino transferase, AST, and LDH are cytoplasmic, and the rise in their serum levels is attributed to damaged structural integrity of the liver and as a result these enzymes are released into the blood circulation after the rupture of the plasma membranes [66, 90].…”

Section: Discussionsupporting

confidence: 92%

“…Present in the cells in both the reduced (GSH) and oxidized (GSSG) forms, but because of the action of the NADPH-dependent enzyme GR, the cellular content of glutathione is predominantly in favour of GSH under normal physiologic conditions [108]. In agreement with previous reports [6, 7, 88, 89], the current study revealed that t -BHP treatment resulted in a reduction in GSH levels both in the erythrocytes and liver, while GSSG level was only increased in the liver. The oxidation of GSH to GSSG is a sensitive marker of oxidative stress and under condition of increased stress, the GSH : GSSG ratio decreases either due to increased GSSG or decreased GSH levels [109].…”

Section: Discussionsupporting

confidence: 89%

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Protective Effects of Rooibos (Aspalathus linearis) and/or Red Palm Oil (Elaeis guineensis) Supplementation ontert-Butyl Hydroperoxide-Induced Oxidative Hepatotoxicity in Wistar Rats

Ajuwon

Katengua-Thamahane

Rooyen

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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The possible protective effects of an aqueous rooibos extract (Aspalathus linearis), red palm oil (RPO) (Elaeis guineensis), or their combination on tert-butyl-hydroperoxide-(t-BHP-)induced oxidative hepatotoxicity in Wistar rats were investigated. tert-butyl hydroperoxide caused a significant (P < 0.05) elevation in conjugated dienes (CD) and malondialdehyde (MDA) levels, significantly (P < 0.05) decreased reduced glutathione (GSH) and GSH : GSSG ratio, and induced varying changes in activities of catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase in the blood and liver. This apparent oxidative injury was associated with histopathological changes in liver architecture and elevated levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH). Supplementation with rooibos, RPO, or their combination significantly (P < 0.05) decreased CD and MDA levels in the liver and reduced serum level of ALT, AST, and LDH. Likewise, changes observed in the activities of antioxidant enzymes and impairment in redox status in the erythrocytes and liver were reversed. The observed protective effects when rooibos and RPO were supplemented concomitantly were neither additive nor synergistic. Our results suggested that rooibos and RPO, either supplemented alone or combined, are capable of alleviating t-BHP-induced oxidative hepatotoxicity, and the mechanism of this protection may involve inhibition of lipid peroxidation and modulation of antioxidants enzymes and glutathione status.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 89%

Protective Effects of Rooibos (Aspalathus linearis) and/or Red Palm Oil (Elaeis guineensis) Supplementation ontert-Butyl Hydroperoxide-Induced Oxidative Hepatotoxicity in Wistar Rats

Ajuwon

Katengua-Thamahane

Rooyen

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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“…Results from this study confirmed t-BHP-induced hepatotoxic effects as shown by the significant increase in the activity of ALT, AST and LDH in the serum of t-BHPtreated rats. These observations are in accordance with those obtained by previous studies [66,[87][88][89]. Alanine amino transferase, AST, and LDH are cytoplasmic, and the rise in their serum levels is attributed to damaged structural Evidence-Based Complementary and Alternative Medicine 13 integrity of the liver and as a result these enzymes are released into the blood circulation after the rupture of the plasma membranes [66,90].…”

Section: Discussionsupporting

confidence: 81%

Protective Effects of Rooibos (Aspalathus Linearis) and/or Red Palm Oil (Elaeis Guineensis) Supplementation on Tert-Butyl Hydroperoxide-Induced Oxidative Hepatotoxicity in Wistar Rats

Ajuwon

Katengua-Thamahane

Rooyen

et al. 2012

Free Radical Biology and Medicine

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The possible protective effects of an aqueous rooibos extract (Aspalathus linearis), red palm oil (RPO) (Elaeis guineensis), or their combination on tert-butyl-hydroperoxide-(t-BHP-)induced oxidative hepatotoxicity in Wistar rats were investigated. tert-butyl hydroperoxide caused a significant ( < 0.05) elevation in conjugated dienes (CD) and malondialdehyde (MDA) levels, significantly ( < 0.05) decreased reduced glutathione (GSH) and GSH : GSSG ratio, and induced varying changes in activities of catalase, superoxide dismutase, glutathione peroxidase, and glutathione reductase in the blood and liver. This apparent oxidative injury was associated with histopathological changes in liver architecture and elevated levels of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH). Supplementation with rooibos, RPO, or their combination significantly ( < 0.05) decreased CD and MDA levels in the liver and reduced serum level of ALT, AST, and LDH. Likewise, changes observed in the activities of antioxidant enzymes and impairment in redox status in the erythrocytes and liver were reversed. The observed protective effects when rooibos and RPO were supplemented concomitantly were neither additive nor synergistic. Our results suggested that rooibos and RPO, either supplemented alone or combined, are capable of alleviating t-BHP-induced oxidative hepatotoxicity, and the mechanism of this protection may involve inhibition of lipid peroxidation and modulation of antioxidants enzymes and glutathione status.

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“…In hepatocytes, t-BHP is metabolized by CYP450 into free radical intermediates such as t -butoxyl and t -methyl radicals [91], which induce lipid peroxidation and glutathione (GSH) depletion resulting in organelle damage and cell death [92]. Hence, t-BHP-induced hepatotoxicity model is widely used for evaluating merits of Nrf2-ARE signaling in hepatocytes.…”

Section: Role Of Nrf2 In Chemical-induced Liver Injurymentioning

confidence: 99%

Naturally Occurring Nrf2 Activators: Potential in Treatment of Liver Injury

Jadeja

Upadhyay

Devkar

et al. 2016

Oxidative Medicine and Cellular Longevity

107

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Oxidative stress plays a major role in acute and chronic liver injury. In hepatocytes, oxidative stress frequently triggers antioxidant response by activating nuclear erythroid 2-related factor 2 (Nrf2), a transcription factor, which upregulates various cytoprotective genes. Thus, Nrf2 is considered a potential therapeutic target to halt liver injury. Several studies indicate that activation of Nrf2 signaling pathway ameliorates liver injury. The hepatoprotective potential of naturally occurring compounds has been investigated in various models of liver injuries. In this review, we comprehensively appraise various phytochemicals that have been assessed for their potential to halt acute and chronic liver injury by enhancing the activation of Nrf2 and have the potential for use in humans.

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Evaluation of hepatotoxicity and oxidative stress in rats treated with tert-butyl hydroperoxide

Cited by 26 publications

References 38 publications

Protective Effects of Rooibos (Aspalathus linearis) and/or Red Palm Oil (Elaeis guineensis) Supplementation ontert-Butyl Hydroperoxide-Induced Oxidative Hepatotoxicity in Wistar Rats

Protective Effects of Rooibos (Aspalathus linearis) and/or Red Palm Oil (Elaeis guineensis) Supplementation ontert-Butyl Hydroperoxide-Induced Oxidative Hepatotoxicity in Wistar Rats

Protective Effects of Rooibos (Aspalathus Linearis) and/or Red Palm Oil (Elaeis Guineensis) Supplementation on Tert-Butyl Hydroperoxide-Induced Oxidative Hepatotoxicity in Wistar Rats

Naturally Occurring Nrf2 Activators: Potential in Treatment of Liver Injury

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