Evaluation of hepatoprotective potential of Erythrina indica leaves against antitubercular drugs induced hepatotoxicity in experimental rats

Abstract

BackgroundErythrina indica Lam. traditionally used in the treatment of laxative, diuretic, worm infestation, liver ailment and joints pain.ObjectiveTo evaluate the antihepatotoxic potential of Erythrina indica against isoniazid (INH) and rifampicin (RIF) induced hepatotoxicity in rats.Methods and materialLiver toxicity was induced by antitubercular drugs (INH+ RIF) at dose level of 50 mg/kg each, p.o for 28 days. 50% methanolic extract of Erythrina indica (100 and 200 mg/kg) were administered orally once daily for 28 days. The hepatoprotective activity was assessed using various biochemical parameters SGOT, SGPT, ALP, bilirubin, total protein, albumin and LDH. Meanwhile, in vivo antioxidant activities as SOD, CAT, GSH and, LPO were measured in liver homogenate also histological examinations were carried out to assess hepatoprotective activity.Statistical analysis usedThe values were subjected to one way analysis of variance (ANOVA) followed by Tukey multiple compare test. Results were considered statistically significant when P < 0.05.ResultsObtained results demonstrated that the treatment with Erythrina indica (E. indica) significantly prevented drug induced increase in serum levels of hepatic enzymes. Furthermore, Erythrina indica significantly reduced the lipid peroxidation (P < 0.01 tp P < 0.001) in the liver tissue and restored activities of defense antioxidant enzymes GSH (2.15 ± 0.08 to 2.48 ± 0.99; P < 0.05), SOD (2.69 ± 0.752 to 3.712 ± 0.056; P < 0.05 to P < 0.01) and CAT (10.20 ± 0.58 to 12.59 ± 0.42; P < 0.05 to P < 0.001) towards normal. Histopathology of liver tissue showed that Erythrina indica attenuated the hepatocellular necrosis, regeneration and repair of cells toward normal.ConclusionThe results of this study strongly indicate the protective effect of Erythrina indica against liver injury which may be attributed to its hepatoprotective activity, and there by scientifically support its traditional use.

Hepatoprotective Activity of Melittin on Isoniazid and Rifampicin Induced Liver Damage in Male Albino Rats

“…Despite tremendous advances in modern medicine, the prevention and treatment of liver diseases such as chronic hepatitis, cirrhosis, fibrosis, and hepatocellular carcinoma remain serious health complications in the world today [1]. The unique metabolic function and relationship of the liver to the gastrointestinal tract makes it an important target of toxicity to xenobiotics, hepatotoxins, environmental pollutants and absorbed drugs [2].…”

In-vivo Hepatoprotective and Antioxidant Activities of Sphaeranthus amaranthoides Burm.f. Against Anti-Tubercular Drugs Induced Hepatotoxicity in Rats

“…The animals were killed on the 15 th day after the dose of Isoniazid -Rifampicin administration. Rats will have free access to food and drinking water during the study [9].…”

Promising Antioxidant Activity of Erythrina Genus: An Alternative Treatment for Inflammatory Pain?

“…In turn, this can impact the modulation of the synthesis and release of inflammatory mediators, such as prostaglandins, nitric oxide, and cytokines such as IL-1 and TNF α [ 19 ]. Docking studies shows that phaseollin of Erythrina variegata has the best fitness score against the COX-1 which is 56.64 and 59.63 for COX-2 enzyme [ 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 ]. However, is required to delve into the possible mechanisms of action, as well as the phytoconstituents and their relationship with the biological activity [ 58 ].…”

“…Inhibition of lipoxygenase and xanthine oxidase, enzymes that participate in the production of reactive oxygen species and pro-inflammatory agents were other tests used [ 55 , 56 , 70 ]. Likewise, the decrease in lipid peroxidation (TBARS) and the inhibition of NO were used to evaluate the antioxidant properties [ 52 , 53 , 71 , 72 ]. Among the compounds responsible for these activities, Eryvarin H, Abyssinone V, mildbone, mildbenone, 7,3′-dihydroxy-4′-methoxy-5′-(3 methylbut-2-enyl)flavanone, erylivingstone H, 7,3′,4′-trihydroxyflavanone, trans-3,4,2′,4′-tetrahydroxychalcone, Eryvarin J and erycrisagallin [ 46 , 55 , 65 , 68 , 73 , 74 , 75 ].…”

“…Additionally, a significant decrease in the hepatic tissue content of TNF-α was demonstrated [ 71 ]. Likewise, the GSH, SOD, and CAT content significantly increased ( p < 0.05 to p < 0.001) in the groups treated with the methanol extract of E. indica leaves [ 52 ]. All this compared with silymarin a potent antioxidant.…”