Evaluation of hepatic changes and local and systemic immune responses in goats immunized with recombinant Peroxiredoxin (Prx) and challenged with Fasciola hepatica

Mendes, Ricardo E.; Pérez-Écija, Alejandro; Zafra, R.; Buffoni, L.; Martı́nez-Moreno, A.; Dalton, John P.; Mulcahy, Grace; Pérez, J.

doi:10.1016/j.vaccine.2010.01.055

Cited by 47 publications

(33 citation statements)

References 39 publications

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“…This is an indication that the expected quantitative reactions of these immune cells to non-self antigens in the mature males and females, and in the kids of both local and Saanen goats might be similar. This includes allergic reactions (McEwen 1992;Rao 2006), reactions to helminthes infestation (McEwen 1992;Mendes et al 2010), phagocytosis and opsonization reactions to different organisms (Rooijakkers et al 2005;Rao 2006;Al-Busaidi et al 2008), and B or T lymphocytes responses to various antigens (Rao 2006;De Marchi et al 2007;Costa et al 2009). The significant high mean blood-CD8 cell count in local mature males compared to the lower means of the other four groups of goats (p<0.05) could indicate a higher immune potential in the local mature males in resisting viral, intracellular infections and in higher protection against neoplastic diseases (Rao 2006;Ponti et al 2008).…”

Section: Resultsmentioning

confidence: 99%

Preliminary comparison of different immune and production components in local and imported Saanen goats reared under a sub-tropical environment

Barbour

Itani

Sleiman

et al. 2011

Trop Anim Health Prod

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Three objectives were included in this research work. The first objective compared different immune components in healthy mature males, mature females, and female kids of local and imported Saanen goats, reared under a sub-tropical environment. The significantly differing immune components were the blood monocyte percent, blood CD8 count, and the total white blood cell count. The second objective compared the performance of Saanen versus local does. The means of the milk yield and prolificacy of the imported Saanen does were significantly higher than those of the local does (p<0.05). The third objective compared the immune responses (hemagglutination-HA titers) and complement fixation (CF) titers in mature does of the two breeds to chicken red blood cells (c-RBC). The HA titers showed a significant seroconversion only in imported Saanen (p<0.05) but not in local does; however, the CF titers increased significantly at 4 weeks following priming with c-RBC in local (p<0.05) but not in the imported Saanen does. The impact of the differences in blood immune components and responses to antigens in the compared goats on protection potential against prevalent diseases in the sub-tropical zone of the eastern Mediterranean countries is discussed.

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Section: Resultsmentioning

confidence: 99%

Preliminary comparison of different immune and production components in local and imported Saanen goats reared under a sub-tropical environment

Barbour

Itani

Sleiman

et al. 2011

Trop Anim Health Prod

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show abstract

“…Although not biochemically characterized, FhPrx1b (which is 99% identical in sequence to FgPrx1b) has a weakly predicted mitochondrial transit peptide of 24 residues at its N-terminus, and FhPrx1a does not (71). FhPrx1a has demonstrated efficacy as a vaccine antigen in an animal model (161), whereas an FgPrx1b-based vaccine has failed in a separate animal trial (211).…”

Section: Prxs In Helminthsmentioning

confidence: 99%

Peroxiredoxins in Parasites

Gretes

Poole²,

Karplus³

2012

Antioxidants & Redox Signaling

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“…Despite intensive research efforts, progress in the development of effective vaccine for the control of F. hepatica and F. gigantica infection has been relatively slow (Mendes et al 2010). Several parasite proteins with a potential to induce protective response in the host have been identified, but the protection conferred by these molecules in different hosts has been shown to vary in different assays.…”

Section: Discussionmentioning

confidence: 99%

“…Fasciolosis is endemic to several parts of India and is one of the major constraints in buffalo productivity (Sanyal 2001). Progress in the development of effective vaccine for Fasciola hepatica and F. gigantica control in ruminants has been relatively slow, despite intensive research efforts (Mendes et al 2010). Several parasite molecules have been identified with a potential to induce a protective immune response in the laboratory and large animals, but the protection conferred to the natural host is variable.…”

Section: Introductionmentioning

confidence: 99%

Vaccination of buffaloes with Fasciola gigantica recombinant glutathione S-transferase and fatty acid binding protein

et al. 2011

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Fasciola gigantica, causative agent of tropical fasciolosis, inflicts substantial economic losses on the livestock industry, affecting severely buffalo productivity in the tropical countries. Very few vaccination trials with different target antigens against F. gigantica infection have been conducted in this host. Present study describes a vaccination trial in buffaloes with F. gigantica recombinant glutathione S-transferase and fatty acid binding protein. The two recombinant proteins were expressed in Escherichia coli and evaluated for their immunoprophylactic potential in buffalo calves, using montanide 70 M-VG, a mineral oil-based adjuvant, for delivering the antigens. Buffalo calves were distributed in three groups, with group I, II and III calves immunized with recombinant glutathione S-transferase, fatty acid binding protein and a cocktail of these two antigens, respectively. Immunization of the calves evoked a mixed IgG1 and IgG2 antibody response. Present vaccination trial in these animals achieved a maximum protection level of 35%, when the two antigens were used in combination. Eosinophils were measured in both immunized and non-immunized challenge control animals, which showed a steady increase in their count in response to immunization with both the antigens and infection with F. gigantica, respectively.

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Evaluation of hepatic changes and local and systemic immune responses in goats immunized with recombinant Peroxiredoxin (Prx) and challenged with Fasciola hepatica

Cited by 47 publications

References 39 publications

Preliminary comparison of different immune and production components in local and imported Saanen goats reared under a sub-tropical environment

Preliminary comparison of different immune and production components in local and imported Saanen goats reared under a sub-tropical environment

Peroxiredoxins in Parasites

Vaccination of buffaloes with Fasciola gigantica recombinant glutathione S-transferase and fatty acid binding protein

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