Evaluation of hematological and biochemical activity of Bauhinia strychnifolia Craib leaves ethanol extracts in normal albino rats

Kwansang, Juthaporn; Damjuti, Watchara; Siriwon, Natnaree

doi:10.5897/ajpp2020.5208

Cited by 2 publications

(2 citation statements)

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“…Only two investigations on antidiabetic activity have been published. In vitro, ethanolic extracts of BC inhibited AG, leading to decreased glucose [ 19 ] and cholesterol and triglyceride in the blood circulation of albino rats [ 20 ]. In addition, the two compounds 3,5,7,3′,5′-pentahydroxy-flavanonol-3-O- α -L-rhamnopyranoside and 3,5,7-trihydroxychromone-3-O-α-L-rhamnopyranoside that were extracted from the stems of BC with ethanol displayed potent inhibitory activity against cancer and AG [ 17 , 19 ] However, further glucose-lowering actions of the stems of BC have not been explored, and the principal bioactive components remain unidentified.…”

Section: Introductionmentioning

confidence: 99%

Alpha-glucosidase inhibitory activities of astilbin contained in Bauhinia strychnifolia Craib. stems: an investigation by in silico and in vitro studies

Eawsakul

Ongtanasup

Ngamdokmai

et al. 2023

BMC Complement Med Ther

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Introduction Bioactive compounds from traditional medicines are good alternatives to standard diabetes therapies and may lead to new therapeutic discoveries. The stems of Bauhinia strychnifolia Craib. (BC) have a possible antihyperglycemic effect; However, the extraction of astilbin from BC has never been recorded in alpha-glucosidase inhibitory activities. Methods Using liquid chromatography–mass spectrometry (LC–MS/MS), 32 compounds were detected in the BC extract. The screening was based on peak area. Seven compounds found. PASS recognized all seven compounds as potential alpha-glucosidase (AG) inhibitors. Astilbin and quercetin 3-rhamnoside were the most likely inhibitors of AG. Arguslab, AutoDock, and AutoDock Vina investigated the binding of the two compounds and AG. The binding stability was confirmed by molecular dynamics (MD). In addition, the optimum solvent extraction was studied via CosmoQuick, and extracts were examined with 1H-NMR prior to testing with AG. Results All three software programs demonstrated that both compounds inhibit AG more effectively than acarbose. According to the sigma profile, THF is recommended for astilbin extraction. The BC extract with THF showed outstanding AG inhibitory action with an IC50 of 158 ± 1.30 µg mL-1, which was much lower than that of the positive control acarbose (IC50 = 190 ± 6.97 µg mL-1). In addition, astilbin from BC was found to inhibit AG strongly, IC50 = 22.51 ± 0.70 µg mL-1 through the extraction method of large-scale astilbin with THF has the best extraction capacity compared to other solvents, hence the initial stage of extraction employs THF to extract and precipitate them with ethyl acetate and water. Conclusion In silico and in vitro studies reveal that astilbin inhibits AG and is superior to acarbose, validating its promise as an AG inhibitor. Overall, astilbin was the most bioactive component of BC for antidiabetic action.

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Section: Introductionmentioning

confidence: 99%

Alpha-glucosidase inhibitory activities of astilbin contained in Bauhinia strychnifolia Craib. stems: an investigation by in silico and in vitro studies

Eawsakul

Ongtanasup

Ngamdokmai

et al. 2023

BMC Complement Med Ther

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“…33 However, contrary to previous studies, rats fed LS ethanol extract at a dose of 125 mg/bw for 21 days cholesterol, TG statistically increased (p<0.05). 34 Altogether, LS capsule was safe and might be utilized as an alternate herb in detoxifying, blood sugar control, and lipid profile management. Moreover, LS also reduced creatinine levels which are beneficial to the kidneys.…”

Section: Discussionmentioning

confidence: 98%

Safety Assessment of Oral Lysiphyllum strychnifolium Aqueous Extract in Healthy Volunteers

Jumpa ngern,

Tungsukruthai,

Kamalashiran

et al. 2024

Pharmacogn J.

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Background: Lysiphyllum strychnifolium (LS), widely known as Ya nang daeng in Thailand, is a traditional herbal remedy that has long been used to promote health and treat diverse health conditions, especially detoxification, by alleviating the severity of symptoms and lowering the risks associated with toxic exposures. Although it is extensively used in Thailand, human safety studies have been lacking. Thus, this study aimed to examine the safety of using LS capsules in healthy participants through a Phase I clinical trial. Objective: This study aimed to investigate the safety of aqueous extract of LS in twenty-four healthy Thai participants. Method: The participants were received 1,000 mg of LS aqueous extract each morning before their meals for seven days. All participants were examined safety assessment including history taking, physical examination, and laboratory tests at day 0, 8 and 14 (follow-up). Results: The findings showed that there were no significant side effects or abnormalities found during the history taking, physical examination, or laboratory evaluation. Particularly, when compared to baseline, participants who received LS experienced statistically significant reductions in blood sugar, triglyceride, LDL cholesterol, and creatinine (P < 0.05), but still within normal ranges. Conclusions: Dietary supplementation with 1,000 mg of LS aqueous extract per day may have a beneficial effect on blood sugar and cholesterol management while remaining safe for healthy people.

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Evaluation of hematological and biochemical activity of Bauhinia strychnifolia Craib leaves ethanol extracts in normal albino rats

Cited by 2 publications

References 8 publications

Alpha-glucosidase inhibitory activities of astilbin contained in Bauhinia strychnifolia Craib. stems: an investigation by in silico and in vitro studies

Alpha-glucosidase inhibitory activities of astilbin contained in Bauhinia strychnifolia Craib. stems: an investigation by in silico and in vitro studies

Safety Assessment of Oral Lysiphyllum strychnifolium Aqueous Extract in Healthy Volunteers

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