Evaluation of Hawaiian Green Turtles (Chelonia Mydas) for Potential Pathogens Associated With Fibropapillomas

Aguirre, A. Alonso; Balazs, George H.; Zimmerman, Brian; Spraker, Terry R.

doi:10.7589/0090-3558-30.1.8

Cited by 89 publications

(57 citation statements)

References 13 publications

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“…Solitary or multiple tumors were found in all regions of the body, including lines of suture adjacent to the dermal shields. The formations are described by other authors in the same way in the turtles included in this work (Jacobson et al 1989, Norton et al 1990, Aguirre et al 1994, 1998, 2002, Brooks et al 1994, Adnyana et al 1997, Work et al 2004.…”

Section: Discussionmentioning

confidence: 56%

Hematological and histopathological evaluation of wildlife green turtles (Chelonia mydas) with and without fibropapilloma from the north coast of São Paulo State, Brazil

Zwarg

Rossi²,

Sanches³

et al. 2014

Pesq. Vet. Bras.

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Section: Discussionmentioning

confidence: 56%

Hematological and histopathological evaluation of wildlife green turtles (Chelonia mydas) with and without fibropapilloma from the north coast of São Paulo State, Brazil

Zwarg

Rossi²,

Sanches³

et al. 2014

Pesq. Vet. Bras.

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“…However, Vibrio spp. are also apparently common commensals in cloacal flora of green turtles from Hawaii with and without FP (Aguirre et al 1994b). The role of Vibrio spp.…”

Section: Discussionmentioning

confidence: 99%

Bacteraemia in free-ranging Hawaiian green turtles Chelonia mydas with fibropapillomatosis

Work

Balazs²,

Wolcott³

et al. 2003

Dis. Aquat. Org.

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Past studies of free-ranging green turtles Chelonia mydas with fibropapillomatosis (FP) in Hawaii have shown that animals become immunosuppressed with increasing severity of this disease. Additionally, preliminary clinical examination of moribund turtles with FP revealed that some animals were also bacteraemic. We tested the hypothesis that bacteraemia in sea turtles is associated with the severity of FP. We captured free-ranging green turtles from areas in Hawaii where FP is absent, and areas where FP has been endemic since the late 1950s. Each turtle was given an FP severity score ranging from 0 (no tumours) to 3 (severely affected). A fifth category included turtles that were stranded ashore and moribund with FP. We found that the percentage of turtles with bacteraemia increased with the severity of FP, and that the majority of bacteria cultured were Vibrio spp. Turtles with severe FP were more susceptible to bactaeremia, probably in part due to immunosuppression. The pattern of bacteraemia in relation to severity of disease strengthens the hypothesis that immunosuppression is a sequel to FP.

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“…Histologically, fibropapillomas are characterized by benign papillary epidermal hyperplasia supported on broad stalks of proliferating fibrovascular stroma (Lucke 1938, Smith & Coates 1938, 1939, Schlumberger & Lucke 1948, Jacobson et al 1989, Harshbarger 1991, Aguirre et al 1994, Williams et al 1994. Agents known to cause similar proliferative cutaneous lesions in other species, including chemical carcinogens, ultraviolet light, oncogenic viruses, and metazoan parasites, have been proposed as possible causes of GTFP and have been reviewed by Sundberg (1991) and Herbst (1994).…”

Section: Introductionmentioning

confidence: 99%

“…Jacobson et al 1989, Hoffman & Wells 1991. The presence of spirorchid trematode ova in the vasculature of many fibropapillomas (Smith & Coates 1939, Harshbarger 1984, Norton et al 1990, Jacobson et al 1991, Aguirre et al 1994., E. Greiner pers. comm.)…”

Section: Introductionmentioning

confidence: 99%

Experimental transmission of green turtle fibropapillomatosis using cell-free tumor extracts

et al. 1995

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Green turtle fibropapillomatosis (GTFP), characterized by multiple benign fibroepithelial tumors on the skin and eyes, has become a growing threat to green turtle Chelonja mydas populations worldwide. The cause of GTFP 1s unknown, but a viral etiology is suspected. This study investigated whether GTFP could be experimentally transmitted to young captive-reared green turtles using cell-free fibropapilloma extracts prepared from free-ranging turtles with spontaneous disease Turtles raised from eggs collected from 4 separate clutches in the wild were assigned to 4 expenmental groups and 1 control group. For each experiment a crude homogenate (33 % w/v) was prepared from fibropapillomas removed from a free-ranging turtle with spontaneous disease. The crude tumor homogenates were freeze-thawed and centrifuged to yield cell-free extracts that were used (both filtered and unaltered) for inoculation. Recipients were inoculated by intradermal injection or by scarification; control turtles were not treated but \yere housed with treated turtles. Fibropapillomas developed in all 12 turtles receiving 3 of the 4 tumor extracts, and were first detected between 15 and 43 wk post inoculation. Both filtered and unfiltered tumor extracts successfully induced tumor development. During the 10 and 12 mo monitoring periods. fibropapillomas did not develop in control turtles or in any turtles inoculated with the fourth tumor extract. Although 2 sets of experiments were performed 8 wk apart, most of the tumors in both sets became evident simultaneously after water temperatures rose Experimental tumors were h~stologically i n d~s -tinguishable from spontaneous fibropapillomas found In free-living turtles but lacked evidence of endoparasites. Scattered foci of epidermal degeneration were found in most sections of experimentally induced fibropapillomas and within some sections taken from donor turtles. Electron rnicroscopy revealed virus-like particles conforming in size, morphology, and intranuclear location with herpesvirus. Negativestaining electron microscopy of transmission-positive tumor extracts failed to demonstrate intact virus particles. This study demonstrates that the etiology of GTFP is an infectious filterable subcellular agent. The herpesvirus identified in this study is 1 possible candidate for the etiology of GTFP.

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Evaluation of Hawaiian Green Turtles (Chelonia Mydas) for Potential Pathogens Associated With Fibropapillomas

Cited by 89 publications

References 13 publications

Hematological and histopathological evaluation of wildlife green turtles (Chelonia mydas) with and without fibropapilloma from the north coast of São Paulo State, Brazil

Hematological and histopathological evaluation of wildlife green turtles (Chelonia mydas) with and without fibropapilloma from the north coast of São Paulo State, Brazil

Bacteraemia in free-ranging Hawaiian green turtles Chelonia mydas with fibropapillomatosis

Experimental transmission of green turtle fibropapillomatosis using cell-free tumor extracts

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