Evaluation of Haematological and Biochemical Activity of Thai Traditional Antinausea Remedy in Normal Albino rats

Abstract

The present study was aimed to evaluate the effect of Thai Traditional anti-nausea remedy against haematological and biochemical effects in animals in the acute toxicity and sub-acute toxicity phase. Twenty-two male and female rats were divided into four groups with equal numbers of sex, each group were respectively received distilled water, 500, 1000 and 2000 mg/kg body weight as a single dose by gavage. While the experiment in the sub-acute phase, eighteen male and female rats were divided into three groups with equal numbers of sex. Each group were respectively given distilled water, 500 and 1000 mg/kg body weight once a day for the duration of the experiment until collecting blood samples. The blood samples were collected for haematological: Red Blood Cell (RBC), Haemoglobin (HGB), Hematocrit (HCT), Mean Corpuscular Volume (MCV), White Blood Cell (WBC), Neutrophil (NEU), Lymphocyte (LYMPH), Monocyte (MONO), Eosinophil (EO), Basophil (BASO) and biochemical analysis; Glucose (GLU), Blood Urea Nitrogen (BUN), Creatinine (CREA), Cholesterol (CHOL) and Triglyceride (TG). The results showed that the remedy caused behavioural adverse effects, but no mortality observed. In the acute toxicity phase, single oral administration of anti-nausea remedy increased mean difference compared to the control group on Creatinine level and decreased in AST level and ALP level (P<0.05). In the subacute study, daily oral administration of the remedy did not result in death or changes in the body weight and food consumption but also showed comparable results on renal function marker including increasing of BUN levels and decreasing of AST level and ALP level (P<0.05). In haematological and some biochemical analyses showed few disadvantageous effects. Based on the results obtained, although there were some changes in blood chemistry values, these were small changes not higher than reference values, indicating that the remedy was safe to take for a short period of time.