Evaluation of Gravitropism in Non-seed Plants

Zhang, Yuzhou; Li, Lanxin; Friml, Jiřı́

doi:10.1007/978-1-0716-1677-2_2

Cited by 1 publication

(2 citation statements)

References 14 publications

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“…These suggest that FER contributes to RALF1-triggered rapid responses, consistent with previous studies showing that FER mediates the rapid net H + influx induced by RALF33 ( 34 ) and the long-term medium pH changes triggered by RALF1 ( 9 ). To track the long-term growth response, we recorded seedlings transferred to media containing various concentrations of RALF1 on a high-throughput vertical scanner ( 35 ). We found that the fer-4 mutants were completely insensitive to 0.1 to 10 µM of RALF1 ( SI Appendix , Fig.…”

Section: Resultsmentioning

confidence: 99%

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RALF1 peptide triggers biphasic root growth inhibition upstream of auxin biosynthesis

Chen

Alotaibi

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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Plant cell growth responds rapidly to various stimuli, adapting architecture to environmental changes. Two major endogenous signals regulating growth are the phytohormone auxin and the secreted peptides rapid alkalinization factors (RALFs). Both trigger very rapid cellular responses and also exert long-term effects [Du et al., Annu. Rev. Plant Biol. 71, 379–402 (2020); Blackburn et al., Plant Physiol. 182, 1657–1666 (2020)]. However, the way, in which these distinct signaling pathways converge to regulate growth, remains unknown. Here, using vertical confocal microscopy combined with a microfluidic chip, we addressed the mechanism of RALF action on growth. We observed correlation between RALF1-induced rapid Arabidopsis thaliana root growth inhibition and apoplast alkalinization during the initial phase of the response, and revealed that RALF1 reversibly inhibits primary root growth through apoplast alkalinization faster than within 1 min. This rapid apoplast alkalinization was the result of RALF1-induced net H + influx and was mediated by the receptor FERONIA (FER). Furthermore, we investigated the cross-talk between RALF1 and the auxin signaling pathways during root growth regulation. The results showed that RALF-FER signaling triggered auxin signaling with a delay of approximately 1 h by up-regulating auxin biosynthesis, thus contributing to sustained RALF1-induced growth inhibition. This biphasic RALF1 action on growth allows plants to respond rapidly to environmental stimuli and also reprogram growth and development in the long term.

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Section: Resultsmentioning

confidence: 99%

“…As a high-throughput method of growth analysis, we used a vertical flatbed scanner (Epson perfection V370) ( 35 ) with slots for Petri dishes containing 1/2 MS medium with treatments as indicate. Automatic scanning at 1,200 dpi every hour using the AutoIt script was described previously ( 51 ).…”

Section: Methodsmentioning

confidence: 99%