Evaluation of gram negative bacterial contamination in dental unit water supplies in a university clinic in Tabriz, Iran

Pouralibaba, Firoz; Balaei, Esrafil; Kashefimehr, Atabak

doi:10.5681/joddd.2011.021

Cited by 4 publications

(2 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Next, uses in water remediation, to clear up waters contaminated by bacteria, which are often predominantly Gram-negatives, are conceivable, too. For example, dental unit water supplies are most commonly contaminated by Pseudomonas and Legionella among other Gram-negative bacteria [ 129 ]. Aerator water supplies are also most commonly contaminated by Legionella pneumophila and other Gram-negatives [ 130 ], presenting the major source of infection for immunocompromised patients in hospitals.…”

Section: Resultsmentioning

confidence: 99%

Altering Microbiomes with Hydroxyapatite Nanoparticles: A Metagenomic Analysis

Uskoković

Wu²

2022

Materials

View full text Add to dashboard Cite

Hydroxyapatite (HAp), the most abundant biological material among mammals, has been recently demonstrated to possess moderate antibacterial properties. Metagenomics provides a series of tools for analyzing the simultaneous interaction of materials with larger communities of microbes, which may aid in optimizing the antibacterial activity of a material such as HAp. Here, a microbiome intrinsic to the sample of sandy soil collected from the base of an African Natal plum (Carissa macrocarpa) shrub surrounding the children’s sandbox at the Arrowhead Park in Irvine, California was challenged with HAp nanoparticles and analyzed with next-generation sequencing for hypervariable 16S ribosomal DNA base pair homologies. HAp nanoparticles overwhelmingly reduced the presence of Gram-negative phyla, classes, orders, families, genera and species, and consequently elevated the relative presence of their Gram-positive counterparts. Thermodynamic, electrostatic and chemical bonding arguments were combined in a model proposed to explain this selective affinity. The ability of amphiphilic surface protrusions of lipoteichoic acid in Gram-positive bacteria and mycolic acid in mycobacteria to increase the dispersibility of the bacterial cells and assist in their resistance to capture by the solid phase is highlighted. Within the Gram-negative group, the variability of the distal, O-antigen portion of the membrane lipopolysaccharide was shown to be excessive and the variability of its proximal, lipid A portion insufficient to explain the selectivity based on chemical sequence arguments. Instead, flagella-driven motility proves to be a factor favoring the evasion of binding to HAp. HAp displayed a preference toward binding to less pathogenic bacteria than those causative of disease in humans, while taxa having a positive agricultural effect were largely captured by HAp, indicating an evolutionary advantage this may have given it as a biological material. The capacity to selectively sequester Gram-negative microorganisms and correspondingly alter the composition of the microbiome may open up a new avenue in environmental and biomedical applications of HAp.

show abstract

Section: Resultsmentioning

confidence: 99%

Altering Microbiomes with Hydroxyapatite Nanoparticles: A Metagenomic Analysis

Uskoković

Wu²

2022

Materials

View full text Add to dashboard Cite

show abstract

“…Furthermore, within the dental clinic, water spreads as an aerosol, increasing both bacterial spread and the risk of infection to all people in the premises [12,[21][22][23][24]. Oral flora [25] and human pathogens (e.g., Pseudomonas aeruginosa [26], various Legionella species (Legionella pneumophila) [27,28], non-tuberculous Mycobacterium species (Mycobacterium) [29], Helicobacter pylori [30] and other microorganisms including unicellular algae, bacteria, fungi, and protozoa [16,31]) have already been isolated from dental unit water systems [32]. They can coat and colonize almost any material in the dental clinic [33,34], especially the suction tube [16], forming biofilms.…”

Section: Introductionmentioning

confidence: 99%

Estimation and Factors Affecting Water Quality and Sustainability in the Private Dental Sector in Greece

Antoniadou,

Intzes,

Kladouchas

et al. 2023

Preprint

View full text Add to dashboard Cite

Dental healthcare providers, staff and patients are constantly exposed to the risk of infection or cross-infection in the dental office, particularly due to the formation of biofilms within Dental Unit Waterlines (DUWLs). Good water quality, achieved with environmentally friendly means, is essential in ensuring the safe operation of the clinic. The aim of this study was to evaluate the practices and protocols used in dental offices of Metropolitan Athens in Greece with regards to water quality in DUWLs. The questionnaire about water quality was distributed to dental practitioners in 2022 and was completed by 206 dentists. Water supplied to dental units was mainly from the public network (65.0%), with 55.8% using in-office filtration. Most dentists (71.8%) do not know the active substance of the antiseptic used for the DUWLs, while 96.1% reported having an antiseptic reservoir embedded in the dental unit. Daily flushing with a small and a large amount of fluid was practiced in approximately 83% and 61% of dental offices, respectively. Dental associations should raise awareness regarding water quality and maintenance of DUWLs in dental offices, investing in lifelong learning, while implementing protocols and green strategies.

show abstract

Water Contamination Risks at the Dental Clinic

Cicciù

2020

Biology

View full text Add to dashboard Cite

Dental clinics, furnished with an array of specialized equipment, are commonplace, particularly in industrialized countries. Minimizing the risk of infection at the dental practice requires the formulation and implementation of strict protocols. These protocols must address the real risk posed by water contamination, particularly given that water is both integral to the function of some dental equipment, and is typically administered directly to the patient. The water in the dental clinic may be of local origin or from a water main, this can be problematic since the clinician often has little assurance regarding the quality of water reaching the dental chair. Though most modern dental equipment includes self-sterilization protocols, care must be taken that water does not stagnate anywhere in the dental equipment or clinic. The management of water quality at the dental clinic is an important part of respecting the protocols needed to manage the risk of patient infections.

show abstract

Evaluation of gram negative bacterial contamination in dental unit water supplies in a university clinic in Tabriz, Iran

Cited by 4 publications

References 26 publications

Altering Microbiomes with Hydroxyapatite Nanoparticles: A Metagenomic Analysis

Altering Microbiomes with Hydroxyapatite Nanoparticles: A Metagenomic Analysis

Estimation and Factors Affecting Water Quality and Sustainability in the Private Dental Sector in Greece

Water Contamination Risks at the Dental Clinic

Contact Info

Product

Resources

About