Evaluation of Gonozyme, an enzyme immunoassay for the rapid diagnosis of gonorrhea

Abstract

A new indirect enzyme immunoassay (EIA), Gonozyme (Abbott Laboratories), was assessed for rapid detection of gonococcal antigens. A correlation of optic density (OD) readings by EIA with colony counts of

Clinical laboratory applications of monoclonal antibodies

“…The sensitivity and specificity of probe assays appear to be comparable to those generated with a commercial polyclonal ELISA for detection of Neisseria gonorrhoeae. Sensitivities ranging from 80 to 99% and specificities ranging from 86 to 98% have been reported with the Gonozyme assay (105,117,147,163). MAb-based assays for detection of Neisseria gonorrhoeae are currently used as culture confirmation tests.…”

Comparison of Gram stain with DNA probe for detection of Neisseria gonorrhoeae in urethras of symptomatic males

“…However, the expensiveness of this technique dictates that it be clearly demonstrated that probes are significantly more advantageous than conventional techniques before they be put to routine use. An important example might be in the use of nucleic acid probes for the detection of gonorrhea in symptomatic males, in which cases simple Gram stains of urethral smears have been reported to be 89 to 95% sensitive and 94 to 97% specific (1)(2)(3). We decided to compare the Gram stain method with the Gen-Probe system (Pace II; Gen-Probe, San Diego, Calif.) for the detection of gonococcal infection in males with symptoms of urethritis attending our sexually transmitted disease clinics.…”