Evaluation of Glucose Dehydrogenase and Pyrroloquinoline Quinine (pqq) Mutagenesis that Renders Functional Inadequacies in Host Plants

Naveed, Muhammad; Sohail, Younas; Khalid, Nauman; Ahmed, Iftikhar; Mumtaz, Abdul Samad

doi:10.4014/jmb.1501.01075

Cited by 14 publications

(14 citation statements)

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“…The biochemical pathways and genetics of PQQ-GDH synthesis and expression have been studied in many bacteria [32,41,42]. Van Schie et al [42] showed that the synthesis of PQQ-GDH holoenzyme and the PQQ cofactor could be non-coordinated and the control of PQQ-GDH activity by PQQ synthesis maybe widespread among bacteria.…”

Section: Discussionmentioning

confidence: 99%

Mineral Phosphate Solubilization in Burkholderia tropica Involves an Inducible PQQ-Glucose Dehydrogenase

Bernabeu

Garcia

Ferreyra

et al. 2016

BMRJ

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Authors' contributionsThis manuscript is the result of teamwork. Author PRB designed all the experiments and managed the analysis and discussion of results. Author SSG contributed with experiments in flasks and gluconic acid and phosphorous determinations and managed literature searches. Author MLG carried out the supervision of experiments in flasks and bioreactors in relation with enzyme activities measures. Authors GGF and VIG realized all the experiments in bioreactors and enzyme activities measures. Author JLB contributed with the discussion of results, organization and planning of the manuscript.Author MFL developed the experimental design, supervised the experiments and managed the analysis and discussion of results. All authors read and approved the final manuscript. Article InformationABSTRACT Aims: The objective of this work was to provide knowledgement about the mechanism and regulation of the mineral phosphate solubilization in Burkholderia tropica. To this end, the expression of the direct extracellular oxidative pathway in B. tropica was studied using different culture approaches. Bernabeu et al.; BMRJ, 13(4): 1-8, 2016; Article no.BMRJ.24405 2 Study Design: Plate assays and batch cultures in flasks and bioreactor were carried out in this study with B. tropica Mto-293 like target organism. The experiments were achieved at least three times with two repetitions per time. Place and Duration of Study: Departamento de Química, Centro de Investigación y Desarrollo en Fermentaciones Industriales, UNLP, CCT-La Plata-CONICET, between November 2014-2015. Methodology: Qualitative plate assays with different Carbon sources were carried out for the evaluation of Mineral Phosphate Solubilization phenotype. Batch cultures in flasks were carried out with different Carbon, Phosphorus and Nitrogen sources to determine quantitatively soluble phosphorus, gluconic acid and other ketoacids in the supernatants, and also PQQ-linked glucose and gluconate dehydrogenase activities in whole cells. Original Research ArticleCultures with some of the conditions mentioned before were carried out in bioreactor specifically to control pH. Results: This organism was able to produce significant amounts of gluconic acid via the expression of a PQQ-GDH and also showed a significant activity of GaDH. However, the direct oxidative pathway was only observed under conditions of Phosphorus starvation and/or Nitrogen fixation. Conclusion:The Mineral Phosphate Solubilization phenotype for B. tropica can be ascribed to the expression of the direct oxidative pathway which involves the expression of an active PQQ-linked glucose dehydrogenase. Nevertheless, this pathway is not expressed constitutively in this bacterium. Environmental conditions, like low P and N availability, led to an active extracellular glucose oxidation. Therefore, mineral phosphate solubilization in B. tropica involves an inducible pyrroloquinoline quinone-linked glucose dehydrogenase. These findings may contribute to the use of this bacterium as plant growth promoting bacte...

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Section: Discussionmentioning

confidence: 99%

Mineral Phosphate Solubilization in Burkholderia tropica Involves an Inducible PQQ-Glucose Dehydrogenase

Bernabeu

Garcia

Ferreyra

et al. 2016

BMRJ

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“…QAU-92 (Table 1). PCR was carried out according to the procedure described by Naveed et al (2015). The amplified PCR products were sequenced commercially from LGC genomics (Germany) and compared with already published sequences in NCBI GenBank databases.…”

Section: Identification Of Isolate and Amplification Of Pqq Operonmentioning

confidence: 99%

“…To induce mutation in the pqq operon, the pqq biosynthesis "pqqC" was targeted for site directed mutation using PCR based knock out method (Naveed et al 2015) as described for QAU-92. The deletion plasmid of Pseudomonas spp.…”

Section: In Vivo Cloning and Site-specific Mutagenesis And Phosphate Solubilizationmentioning

confidence: 99%

Role of Pyrroloquinoline Quinone in Biocontrol Together with Induced Systemic Resistance: A Novel Resource Trialed for Rice Disease Control

Naveed¹

2021

IJAB

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Plants show strong levels of resistance to an extensive range of pathogens on account of root colonization through plant growth-promoting rhizobacteria (PGPR), namely, induced systemic resistance (ISR). Little is known about bacterial determinants and plant signaling pathways that underpin ISR in cereal crops associated with ISR in dicotyledonous plants. The present study evaluates the potential of Pseudomonas spp. QAU-92 using site directed mutagenesis of the pqqC gene to elicit ISR in rice (Oryza sativa L.) against the fungal pathogen Cochliobolus miyabeanus. The comparison between the wild-type strain and the mutant strain for biochemical attributes, in vitro and in vivo antagonistic activity, carbon source utilization assay and in vivo analyses on rice (cv. C-039) revealed the statistically significant role of Pyrroloquinoline Quinone (PQQ) in plant growth promotion. RT-qPCR analysis revealed that the plant recognition of QAU-92 results in the activation of ethylene (ET) and jasmonic acid (JA) pathways and also shows clear differences in resistance against C. miyabeanus disease compared with the pqqC mutants (QAU92-2). The expression of TF 89 (EBP89), a susceptible gene, as well as the pathogenesis-related protein 1a (PR1a) were much higher in the infected control and pqqC mutant plant than in wild type inoculated plants. Hence, this study is the first of the kind that has investigated the expressional analysis of PQQ against antifungal activity, phosphate solubilization and the induced systemic resistance of QAU-92 against C. miyabeanus in rice. Additionally, PQQ genes may act as a key regulator of PR1a/ET cross-talk and its interference with the fungal manipulation of plants. © 2021 Friends Science Publishers

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“…Novel allyl PQQ combination with chlorogenic acid and methyl gentisate is found effective for hyper pigmentation acknowledged at diminution of skin hyper pigmentation (Gold; jhskincareclinic.co.uk).Loss of PQQ causes a reduction in connective tissue growth and repair proved by Cellular studies on fibroblasts leading to friable skin, loss of elasticity and connective tissue health also used in makeup and creams for aging and skin care etc. PQQ contributes on epidermal water dissipation, skin moisture, texture, viscoelasticity, and reduction in mast cell quantity in the dermis and epidermis and quantity of CD3⁺ T-cells giving improved skin barrier and function (Table 2) [112]. PQQ causing biological aging induced by ultraviolet A UVA in dermal fibroblasts of humans HDFs via ant apoptotic SIRT1-SIRT6-HO-1 and Nrf2 signaling pathways [113].…”

Section: Pqq Role In Skinmentioning

confidence: 99%

The Life History of Pyrroloquinoline Quinone (PQQ): A Versatile Molecule with Novel Impacts on Living Systems

Naveed¹

2016

IJMBOA

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Pyrroloquinoline quinone (PQQ) acting as redox cofactor of Glucose dehydrogenase is orthocyclic antioxidant acting under multifarious environmental stress and rich conditions in prokaryotes as well as eukaryotes. Microbes are the exclusive source of PQQ biosynthesis and for biocatalysis of glucose into gluconic acid and 2-ketogluconic acid by gram positive and negative bacteria. This study focus to describe PQQ biography highlighted its applications acts as biocatalyst by enhanced production of NADH from pqqC, involved in several important mechanisms like bacterial energy transduction by m-ATPase, production of biocontrol substances, growth stimulating activities and DNA repair. PQQ acting as anti-neurological, anti-degenerative, anti-melanogenic and anti-cancer agent due to its antioxidant nature by scavenging free radicals. PQQ is modulator of immunity by CD4 cells count and IL-2, sleep maintenance by PGC-1 alpha pathway and inflammation due to ROS. The mineral phosphate solubilization results in plant growth promotional, biocontrol, antifungal and ISR activities. PQQ nanoparticles used for production of Biofuels cells and sulfonated polymers. It acts as a modulator of diverse signaling pathways like STAT, MAPK, JAK, JNK, P13K/Akt, mTOR, EGFR and Raps for cell proliferation, differentiation, apoptosis, Box translocation and metabolic pathways by phosphorylation of ADP and suppression of reactive oxygen species. It protects from oxidative stress by acting as Alpha AA modulator of lysine metabolism, TrxR1 in selenium metabolism, low density lipoprotein in lipid metabolism, ATP production in Energy, Glucose and carbohydrate metabolism. It has been structurally characterized with bioinformatics tools and future perspectives being molecular modeling and docking for analytical drug design.

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Evaluation of Glucose Dehydrogenase and Pyrroloquinoline Quinine (pqq) Mutagenesis that Renders Functional Inadequacies in Host Plants

Cited by 14 publications

References 32 publications

Mineral Phosphate Solubilization in Burkholderia tropica Involves an Inducible PQQ-Glucose Dehydrogenase

Mineral Phosphate Solubilization in Burkholderia tropica Involves an Inducible PQQ-Glucose Dehydrogenase

Role of Pyrroloquinoline Quinone in Biocontrol Together with Induced Systemic Resistance: A Novel Resource Trialed for Rice Disease Control

The Life History of Pyrroloquinoline Quinone (PQQ): A Versatile Molecule with Novel Impacts on Living Systems

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