Evaluation of genotoxicity of Bacillus mojavensis KJS-3 on culture supernatant for use as a probiotic

Kim, Kang Min; Jung, Taehun; Ok, Seon; Ko, Chan Young; Kang, Jae Seon

doi:10.1007/s13273-012-0010-z

Cited by 5 publications

(5 citation statements)

References 23 publications

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“…Bacillus species are used in many medical, pharmaceutical, agricultural, and industrial processes that take advantage of the bacteria's wide range of physiological characteristics and their ability to produce a host of enzymes, antibiotics, and other metabolites [5,12,13,16]. The rhizosphere, a narrow region of soil that is directly influenced by root secretions, is associated with microbial activity [3].…”

Section: B Mojavensis Kjs-3 a New B Mojavensis Strain Was Initiallymentioning

confidence: 99%

Characterization of Bacillus mojavensis KJS-3 for the Promotion of Plant Growth

Kim¹,

Liu²,

Go³

et al. 2015

Journal of Life Science

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Scientists have recently shown an interest in the characteristics of Bacillus mojavensis strains because of their increasing use in plants as a defense against diseases and mycotoxins. We have shown here that B. mojavensis KJS-3 possesses the typical characteristics of B. mojavensis strains including a strong resistance to high temperatures (≤50°C), tolerance to high salt concentrations (7% NaCl), ethanol tolerance (40% ethanol), and pH range for growth (pH 5-9). B. mojavensis KJS-3 has been used for the production of cyclic lipopeptides including important antifungal substances such as surfactin, iturin, and fengycin. Polymerase chain reaction analysis in this study showed that B. mojavensis KJS-3 can be used for the production of fengycin and the findings of LC-MS/MS analyses suggest that B. mojavensis KJS-3 can be used to produce iturin and surfactin. Antifungal activity analys is confirmed that B. mojavensis KJS-3 has antifungal effects on Botrytis cinerea, Rhizoctonia solani AG-4, Sclerotinia sclerotiorum, and Colletotricum goeosporioides. A microscopy assessment of the roots of wild ginseng plants planted together with B. mojavensis KJS-3 revealed that the roots contained B. mojavensis KJS-3, confirming the bacteria to be a plant growth promoting endophyte (PGPE) which acts against plant diseases and mycotoxins. Our findings lead us to conclude that B. mojavensis KJS-3 can be produced at an industrial level as a microbial pesticide or microbial fertilizer.

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Section: B Mojavensis Kjs-3 a New B Mojavensis Strain Was Initiallymentioning

confidence: 99%

Characterization of Bacillus mojavensis KJS-3 for the Promotion of Plant Growth

Kim¹,

Liu²,

Go³

et al. 2015

Journal of Life Science

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“…typhimurium strains TA97a, TA98, TA100, TA102, and TA1535 and also other LAB B . mojavensis KJS‐3 in TA98, TA100, TA1535, and TA1537, 13 so showed no mutagenic activity under analogous conditions based on the OECD Guidelines.…”

Section: Discussionmentioning

confidence: 96%

“…25 This test could be performed using different S. typhimurium and E. coli testers with and without S9 metabolic activation. Ames test could detect indirect mutants in the presence of S9 metabolic activation; however, in the absence of S9 metabolic activation, it could only detect TA B L E 6 The number of structural and numerical chromosome aberrations induced in CHO-K1 cells with Lactobacillus reuteri 100 exposures for 24 h in the absence of metabolic activation typhimurium strains TA97a, TA98, TA100, TA102, and TA1535 and also other LAB B. mojavensis KJS-3 in TA98, TA100, TA1535, and TA1537, 13 so showed no mutagenic activity under analogous conditions based on the OECD Guidelines.…”

Section: Discussionmentioning

confidence: 99%

“…Bacterial gene mutations, chromosomal aberration assays in mammalian cells, and micronucleus tests in rodents as the fast and trustworthy tests are usually employed for genotoxicity investigation. 13 Studies have shown that in each community, specific species could show better and more beneficial probiotic effects. 14 Therefore, in this study, five Lactobacillus strains as a selective candidate probiotics (SCPs) from the previous study 15 were assessed in terms of genetic toxicity, their potential mutagenic effects according to OECD guidelines (Organization for Economic Cooperation and Development) by performing laboratory evaluations and in vivo studies using mouse models to depict the safety of the strain in question.…”

Section: Introductionmentioning

confidence: 99%

“…Presently, at least three actual tests are used universally to check the genotoxicity of different compounds. Bacterial gene mutations, chromosomal aberration assays in mammalian cells, and micronucleus tests in rodents as the fast and trustworthy tests are usually employed for genotoxicity investigation 13 …”

Section: Introductionmentioning

confidence: 99%

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Safety evaluation of mutagenicity, genotoxicity, and cytotoxicity of Lactobacillus spp. isolates as probiotic candidates

Darbandi

Mirkalantari

Zadeh

et al. 2022

Clinical Laboratory Analysis

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Background: Probiotics promote a healthy balance of gut bacteria and have many beneficial effects on human digestive physiology. Although, few side effects of probiotics have been reported. This study aimed to assess the safety of five probiotic candidate Lactobacillus strains isolated from healthy individuals by examining mutagenicity, genotoxicity, and oral toxic effects. Methods: Five selected candidate probiotic (SCPs) strains were evaluated for genotoxicity (Ames test with Salmonella typhimurium), in vitro mammalian chromosome aberration test and an in vivo mouse micronucleus assay on peripheral blood of mice. To evaluate the oral dose toxicity, BALB/c mice models were treated repeatedly (2000, 1000, and 500 mg/kg body weight /day) for 28-days. Results:The Ames test performed for two S. typhimurium strains TA 98 and TA100 (both in the absence and in the presence of S-9 metabolic activation system) did not show an increase in reverse mutation because of exposure to the SCPs in any of the doses (5.0, 2.5, 1.25, 0.625, and 0.3125 mg/plate). There was no genotoxicity in the SCPs treatment in the vitro chromosome aberration assay with Chinese hamster ovary cells (CHO-K1). In addition, none of the tested strains increased the frequency of micronucleated reticulocytes in reticulocytes, the SCPs with the studied doses caused no substantial variation in the experimental groups compared to the negative control group (p > 0.05). SCPs were not acutely toxic when administered to male and female BALB/c mice by single gavage at (2000, 1000, and 500 mg/kg b.w/day) with no mortality or clinical signs, change in body weight or macroscopic abnormalities were observed in this dose range. Conclusion:As a result, SCPs did not induce mutagenic potential in vitro with bacterial reverse mutation, clastogenicity, and in vivo tests in the ranges of concentrations evaluated in our study.

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Safety Evaluation of a Novel Potentially Probiotic Limosilactobacillus fermentum in Rats

Neto

Oliveira

et al. 2023

Probiotics & Antimicro. Prot.

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Evaluation of genotoxicity of Bacillus mojavensis KJS-3 on culture supernatant for use as a probiotic

Cited by 5 publications

References 23 publications

Characterization of Bacillus mojavensis KJS-3 for the Promotion of Plant Growth

Characterization of Bacillus mojavensis KJS-3 for the Promotion of Plant Growth

Safety evaluation of mutagenicity, genotoxicity, and cytotoxicity of Lactobacillus spp. isolates as probiotic candidates

Safety Evaluation of a Novel Potentially Probiotic Limosilactobacillus fermentum in Rats

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