Evaluation of genetic variability, rust resistance and marker-detection in cultivated Artemisia dracunculus from Iran

Karimi, Ali; Hadian, Javad; Farzaneh, Mohsen; Khadivi, Ali

doi:10.1016/j.gene.2014.10.057

Cited by 22 publications

(15 citation statements)

References 35 publications

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“…The ability to reliably distinguish MAPs populations could be invaluable for their diversity studies, and molecular markers offer an effective approach to manifest genetic diversity based on DNA polymorphisms. ISSR markers have been successfully used for genetic diversity analysis of many other plant species like Salvia miltiorrhiza Bunge , Magnolia officinalis L., Fritillaria imperialis L., Satureja mutica , Artemisia dracunculus L., and Satureja khuzistanica . In the present study, nine highly polymorphic primers ( Table ) were selected from the thirty ISSR primers to amplify the thirteen Perovskia populations, and a total of 119 DNA bands were amplified, 96 of which were polymorphic (the percentage of polymorphic bands (% P ) = 80.7%).…”

Section: Resultsmentioning

confidence: 99%

Genetic and Chemical Diversity in Perovskia abrotanoides Kar. (Lamiaceae) Populations Based on ISSRs Markers and Essential Oils Profile

Pourhosseini

Hadian

Sonboli³

et al. 2018

Chemistry & Biodiversity

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Genetic and the essential oil composition variability among twelve Perovskia abrotanoides populations (PAbPs) growing wild in Iran were assessed by ISSR markers, GC-FID and GC/MS, respectively. Nine selected ISSR primers produced 119 discernible bands, of them 96 (80.7%) being polymorphic. Genetic similarity values among populations ranged between 0.07 and 0.79 which indicated a high level of genetic variation. Polymorphic information content, resolving power and marker index generated by ISSR primers were, 0.31, 6.14, and 3.32, respectively. UPGMA grouped PAbPs into four main clusters. Altogether, 38 chemical compounds were identified in the oils, and a relatively high variation in their contents was found. Camphor (11.9 - 27.5%), 1,8-cineole (11.3 - 21.3%), α-bisabolol (0.0 - 13.1%), α-pinene (5.9 - 10.8%), and δ-3-carene (0.1 - 10.5%) were the major compounds. Oxygenated monoterpenes (32.1 - 35.8%) and monoterpene hydrocarbons (25.7 - 30.4%) were the main groups of compounds in the oils studied. Cluster analysis and principal-component analysis were used to characterize the samples according to oil components. Four main chemotypes were found to be Chemotype I (camphor/1,8-cineol), Chemotype II (1,8-cineole/camphor), Chemotype III (camphor/1,8-cineol/α-bisabolol), and Chemotype IV (camphor/δ-3-carene/α-bisabolol). The information, provided here on P. abrotanoides populations, will be useful to introduce this plant into agricultural systems.

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Section: Resultsmentioning

confidence: 99%

Genetic and Chemical Diversity in Perovskia abrotanoides Kar. (Lamiaceae) Populations Based on ISSRs Markers and Essential Oils Profile

Pourhosseini

Hadian

Sonboli³

et al. 2018

Chemistry & Biodiversity

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“…A large numbers of polymorphic bands (120 bands) were amplified, showing the significance of ISSR marker system for the assessment of genetic diversity in S. khuzistanica populations. ISSR markers have been efficiently used for genetic diversity analysis of several other plant species like Salvia miltiorrhiza Bunge (Song et al 2010), Magnolia officinalis L. (Yu et al 2011), Fritillaria imperialis L. (Badfar-Chaleshtori et al 2012), Satureja rechingeri Jamzad (Hadian et al 2014), and Artemisia dracunculus L. (Karimi et al 2015). A total of 27 different ISSR primers were screened, of which 13 primers with good reproducibility were selected for further study.…”

Section: Resultsmentioning

confidence: 99%

“…ISSR markers have the advantages of relatively low cost, high polymorphism and good reproducibility and exhibit the specificity of microsatellite markers, but need no sequence information for primer synthesis (Uysal et al 2010;Reddy et al 2002). ISSR has been used to analyze genetic diversity and structure of different plant species (Song et al 2010;Yu et al 2011;Badfar-Chaleshtori et al 2012;Hadian et al 2014;Karimi et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of natural populations of medicinally valuable plant Satureja khuzistanica Jamzad based on ISSR markers

et al. 2017

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Satureja khuzistanica Jamzad has been used in pharmaceutical industry because of its interesting pharmacological and biological activities. This study was undertaken to evaluate the level of genetic diversity and differentiation of S. khuzistanica populations. For this purpose, 84 individual plants from seven populations of this species were studied using inter-simple sequence repeat (ISSR) markers. A total of 157 bands were generated by 13 ISSR primers, of which 120 (88.35%) bands were polymorphic. Nei's gene diversity and Shannon information index varied between 0.24 and 0.28 and 0.35 and 0.42, respectively. Analyses of molecular variance detected 90% of variation within populations. Structure analysis showed the highest DK at K = 4, representing the individuals into four groups. In contrast to limited distribution and wild collection, the present study showed high level of genetic diversity in natural populations.

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“…Both ISSR 81 and SRAP markers were suitable for discriminating among the studied individuals, but the SRAP markers were more efficient and preferable. 78 Multiple regression analysis revealed statistically significant association between rust resistance and some molecular markers, which can provide clues for the identification of the individuals with higher rust resistance. RAPD (randomly amplified polymorphic DNA) and ISSR markers were used to characterize Schisandra chinensis with white fruit.…”

Section: Evolution Of Genome Gene and Genotypementioning

confidence: 97%

“…The microsatellites existing in the complete genome sequences would have a direct role in the genome organization, recombination, gene regulation, quantitative genetic variation, and evolution of genes. Microsatellite markers have been characterized for many medicinal plant families and genera, eg, Acanthaceae family, 77 Artemisia genus, 78 Camellia genus, 79 and Chinese jujube. 80 For instance, 11 nuclear SSR loci were used to reveal the relative low genetic diversity of three Camellia taliensis (Da Li tea) populations, three C. sinensis var.…”

Section: Evolution Of Genome Gene and Genotypementioning

confidence: 99%

Genomics and Evolution in Traditional Medicinal Plants: Road to a Healthier Life

Hao

Xiao

2015

Evol Bioinform Online

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Medicinal plants have long been utilized in traditional medicine and ethnomedicine worldwide. This review presents a glimpse of the current status of and future trends in medicinal plant genomics, evolution, and phylogeny. These dynamic fields are at the intersection of phytochemistry and plant biology and are concerned with the evolution mechanisms and systematics of medicinal plant genomes, origin and evolution of the plant genotype and metabolic phenotype, interaction between medicinal plant genomes and their environment, the correlation between genomic diversity and metabolite diversity, and so on. Use of the emerging high-end genomic technologies can be expanded from crop plants to traditional medicinal plants, in order to expedite medicinal plant breeding and transform them into living factories of medicinal compounds. The utility of molecular phylogeny and phylogenomics in predicting chemodiversity and bioprospecting is also highlighted within the context of natural-product-based drug discovery and development. Representative case studies of medicinal plant genome, phylogeny, and evolution are summarized to exemplify the expansion of knowledge pedigree and the paradigm shift to the omics-based approaches, which update our awareness about plant genome evolution and enable the molecular breeding of medicinal plants and the sustainable utilization of plant pharmaceutical resources.

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Evaluation of genetic variability, rust resistance and marker-detection in cultivated Artemisia dracunculus from Iran

Cited by 22 publications

References 35 publications

Genetic and Chemical Diversity in Perovskia abrotanoides Kar. (Lamiaceae) Populations Based on ISSRs Markers and Essential Oils Profile

Genetic and Chemical Diversity in Perovskia abrotanoides Kar. (Lamiaceae) Populations Based on ISSRs Markers and Essential Oils Profile

Genetic diversity of natural populations of medicinally valuable plant Satureja khuzistanica Jamzad based on ISSR markers

Genomics and Evolution in Traditional Medicinal Plants: Road to a Healthier Life

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