Evaluation of Genetic Variability among Three Pistacia Species Using Internal Transcribed Spacer 1 (ITS1) Marker

Abstract

Diversity in Pistacia has been evaluated at all molecular levels using the internal transcribed spacer 1 (ITS1) marker in three species (Pistacia atlantica subsp. atlantica; Pistacia vera and Pistacia terebinthus), and compared with other Pistacia species. Results showed that the ITS amplification and sequencing, followed by phylogenetic analyses, identify the species and confirm their classification, which revealed that it can be used as a marker. Our results suggest that ITS1 analyses might provide a simple and inexpensive approach to validate the species of samples collected from the natural population, where species identification can be difficult, especially if hybrids are present or if the season is not optimal for identifying differences in morphological traits.

Nutraceutical Content and Biological Properties of Lipophilic and Hydrophilic Fractions of the Phytocomplex from Pistacia atlantica Desf. Buds, Roots, and Fruits

“…Pistacia atlantica Desf., also called “Atlas pistachio” or “Mount Atlas mastic tree”, is one of the most diffused species and typical of Algeria, as well as other countries from North Africa and the Middle East [ 2 ]. This tree is suitable for arid and semi-arid areas, especially uplands [ 3 ].…”

“…This substance has been used in culinary applications, such as a flavoring agent, and as a chewing gum component [ 5 ]. Seeds and resin have been also utilized in medicinal preparations against gastrointestinal, liver, splenic, neurological, cardiac, psychological, respiratory, urogenital, and dermatologic disorders [ 2 , 6 , 7 ]. Due to their content of bioactive compounds with potential health benefits, P. atlantica leaves may be also used in phytotherapy, beyond classic recipes, like salads and bread [ 5 , 8 , 9 ].…”