Evaluation of genetic risks of alkylating agents. II. Haemoglobin as a dose monitor

Osterman-Golkar, Siv; Ehrenberg, L.; Segerbäck, Dan; Hällström, Inger Porsch

doi:10.1016/0027-5107(76)90256-6

Cited by 253 publications

(84 citation statements)

References 14 publications

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“…Hemoglobin (Hb) is suitable as a monitor of genotoxic agents becau se of its ready availability and its relati vel y long life spa n, which permits cumulative do ses to be determined (6). Th e measur em ent of Hb adducts ha s been used for monitoring en vironmental factors such as occupational exposures to ethylene oxide (7)(8)(9)(10)(11), propylen e oxid e (9,12), and various components of tobacco smoke (13,14).…”

mentioning

confidence: 99%

Monitoring occupational exposure to styrene from hemoglobin adducts and metabolites in blood.

Christakopoulos¹,

Bergmark²,

Zorcec³

et al. 1993

Scand J Work Environ Health

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mentioning

confidence: 99%

Monitoring occupational exposure to styrene from hemoglobin adducts and metabolites in blood.

Christakopoulos¹,

Bergmark²,

Zorcec³

et al. 1993

Scand J Work Environ Health

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“…Thus, these adducts would be expected to accumulate as a result of chronic exposure; this result was observed ( Figure 3B). After cessation of dosing (after 35 Figure 3B. The rate of disappearance of the adducts in Figure 3B seems to be faster than that observed in Figure 3A.…”

Section: Analysis Of Hemoglobin Adducts Fliowig the Or Adnistration Omentioning

confidence: 70%

Development of Methods to Monitor Exposure to 1-Nitropyrene

El‐Bayoumy¹,

Johnson²,

Roy³

et al. 1994

Environmental Health Perspectives

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On the basis of 32P-postlabeling analysis, treatment of rats with 1-nitropyrene (1-NP) resulted in the formation of multiple DNA adducts in the liver, mammary glands, and peripheral lymphocytes. The one adduct resulting from nitroreduction, N-(deoxyguanosin-8-yl)-1-aminopyrene, constitutes only a minor component among the adducts. In the present study, incubation of calf thymus DNA with mutagenic ring-oxidized metabolites of 1-NP in vitro in the presence and absence of xanthine oxidase also resulted in the formation of multiple adducts. On the basis of their chromatographic behavior, it appears that DNA adducts derived from such metabolites may have been formed in vivo; however, this needs to be confirmed.[3H]1-NP was given to male and female F344 rats and Sprague-Dawley rats by gavage at five dose levels in the range of 0.1 to 1000 pg/kg bw. This led to stable hemoglobin adducts accounting for 0.08 ± 0.05% of the dose (n = 3 rats). The radioactivity associated with hemoglobin following administration of [3H]1-NP was cleared with a half-life of about 14 days, which is faster than that of unmodified erythrocytes in the rat (t1/2= 30 days).Treatment of the hemoglobin with 1% HCI in acetone, to precipitate the globin, released the radioactivity; it was all bound to the heme moiety. The structures of the heme adducts have not been elucidated; yet, because of their stability, they may be useful as dosimeters for human exposure to 1-NP. The results of this study demonstrate the potential of using hemoglobin adducts of 1-NP as dosimeters of uptake and metabolic activation of nitropolynuclear aromatic hydrocarbons (NO2-PAH). These indicators are a prerequisite for cancer risk assessment of N02-PAH. -Environ Health Perspect 102(Suppl 6): 31-37 (1994)

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“…Hydroxyethylated products in hemoglobin are stable, and the stability of alkylated hemoglobin chains is determined by the life span (t) of the erythrocytes (er) (which equals 126 d in man). During long-term exposure (~ter) a steady-state (ss) adduct level is established (18). This steady-state adduct level is related to the average daily increment in the adduct level,…”

Section: Methodsmentioning

confidence: 99%

“…Current exposures can be mon itored through the determination of hydro xyethylated amino acids in hemoglobin (2, 9, 24) for a reliable measur e of the average in vivo dose obtained during the last mon ths of exposure (2,18). Howeve r, the control of work environ-I Department of Radiobiology, Stockholm University, Stockholm , Sweden .…”

mentioning

confidence: 99%

Occupational exposure to ethylene oxide. Relation between in vivo dose and exposure dose.

Osterman-Golkar¹,

Bergmark²

1988

Scand J Work Environ Health

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Evaluation of genetic risks of alkylating agents. II. Haemoglobin as a dose monitor

Cited by 253 publications

References 14 publications

Monitoring occupational exposure to styrene from hemoglobin adducts and metabolites in blood.

Monitoring occupational exposure to styrene from hemoglobin adducts and metabolites in blood.

Development of Methods to Monitor Exposure to 1-Nitropyrene

Occupational exposure to ethylene oxide. Relation between in vivo dose and exposure dose.

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