Evaluation of genetic integrity of tomato seeds during ageing by microsatellite markers

Daniel, I. O.; Adabale, O. W.; Adeboye, Kehinde Adewole; Aladele, E.S.; Oduoye, Olusola Thomas; Adetumbi, J. A.; Boerner, A.

doi:10.1016/j.nigjg.2015.09.001

Cited by 7 publications

(4 citation statements)

References 7 publications

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“…Using artificial seed ageing and DNA profiling of diverse tomato accessions, Daniel et al. (2014) noted that microsatellite markers (SGN‐E111025, SGN‐M15396, SGN‐TGS209, and SGN‐M15617) sequences were associated with seed ageing related changes in genetic integrity at a specific physiological (germinability) benchmark (∼42% seed germinability benchmark). Hence, this germinability threshold of ∼42% can be used as an indicator of genetic changes in tomato seeds.…”

Section: Biotech‐led Profiling and Genetic Enhancement Of Seed Traitsmentioning

confidence: 99%

First the seed: Genomic advances in seed science for improved crop productivity and food security

Dwivedi¹,

Spillane

Lopez

et al. 2021

Crop Science

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Seeds are valuable sources of carbohydrates, lipids, proteins, fibers, minerals, and vitamins. They provide energy and nutrition to germinating seedlings, food to humans, feed to livestock and feedstocks to industry. High-throughput analyses of gene expression in crops has identified many candidate genes associated with seed dormancy, longevity, germination, and vigor. In this review, we cover the latest research focusing on such key seed traits. Transcriptome analyses of time-courses of seed filling have identified sets of genes expressed at different stages of this process. The potential role of epigenetics (including seed-endosperm imprinted genes) in regulating seed development and chemistry is highlighted herein. We also discuss how advances in genomics and seed biology are facilitating the unravelling of associations between seed traits with genebank accessions and gene sequences, including how functional research can accelerate the discovery of allelic variants. Such knowledge of functional effects relating to gene variants is necessary for more efficient and cost-effective management of genetic resources or for redesigning crops with specific seed characteristics. For instance, genebank curators may assess seed viability by monitoring changes in gene expression of biomarker genes in dry seed samples to decide germplasm regeneration and assess genetic integrity of collections by monitoring changes in This article is protected by copyright. All rights reserved. diversity and allele frequencies between samples of same accession stored in genebanks. We highlight that resistance to preharvest sprouting can be enhanced through genomicsassisted breeding in otherwise nondormant rice and wheat cultivars, while pimt, another valuable marker for seed longevity, may be deployed to enhance seed vigor in crops.

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Section: Biotech‐led Profiling and Genetic Enhancement Of Seed Traitsmentioning

confidence: 99%

First the seed: Genomic advances in seed science for improved crop productivity and food security

Dwivedi¹,

Spillane

Lopez

et al. 2021

Crop Science

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“…With the advent of -omics methodologies, a significant in-depth understanding of regulatory mechanisms signaling seed deterioration and invigoration has been gained and applies to crops [37,34]. Changes in specific sequences of highly polymorphic genetic markers in aging rice [38], tomatoes [39] and wheat seeds [40] provide hints of molecular hints on genetic influences behind seed aging.…”

Section: -Omics Of Regulatory Mechanisms For Seed Aging/primingmentioning

confidence: 99%

Biology of Seed Vigor in the Light of -omics Tools

Daniel¹

2017

Advances in Seed Biology

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Seed vigor is a major agronomic trait measurable by seed longevity in storage, germination capacity, and seedling growth in the field. Seed vigor has potential to significantly elevate crop resilience to biotic and abiotic stresses. That is important for crop yields enhancement and other enterprises that involve seeds like plant breeding, research and education, germplasm conservation and the seed trade. With the availability of high precision -omics tools for biological research, lots of investigations are undertaken globally to answer the physiological questions underlying seed germination and invigoration. The increasing -omics datasets constitute important resources for the delivery of new seed vigor markers and advancing new seed vigor manipulation opportunities. There is need to regularly update the knowledge generated from these investigations for the scientific improvement of seed vigor. Thus, this chapter highlights the biological backgrounds involved in the development of seed vigor traits in the light of modern -omics tools. The chapter is sectioned into; 1. Attributes of seed vigor and the -omics sciences; 2. State of -omics-based knowledge on underlying mechanisms of seed vigor; 3. Future perspectives of -omics application to genetic engineering of seed vigor with an insight to the latest technique of genome editing, the CRISPR-Cas9 technology.

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“…Seed aging is a process that causes the quality of seeds to deteriorate, resulting in reduced germination and vigor. This process can occur during seed development, after harvesting, and during storage, and it can be influenced by various environmental factors, such as temperature, humidity, and oxygen availability. − Aged seeds have been reported to cause a decrease in antioxidant systems, disruption of cellular membranes, genetic integrity damage, lipid peroxidation, and protein degradation in seeds . Elevated temperatures have been identified as a significant factor that expedites the aging process of seeds, subsequently diminishing their viability. , The effect of temperature stress on the seed germination rate and vigor has been reported.…”

Section: Introductionmentioning

confidence: 99%

Comparative Proteomic Analysis of Ridge Gourd Seed (Luffa acutangula (L.) Roxb.) during Artificial Aging

Kumsab,

Yingchutrakul,

Simanon

et al. 2024

ACS Omega

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Seed aging is a complicated process influenced by environmental conditions, impacting biochemical processes in seeds and causing deterioration that results in reduced viability and vigor. In this study, we investigated the seed aging process of ridge gourd, which is one of the most exported commercial seeds in Thailand using sequential window acquisition of all theoretical fragment ion spectra mass spectrometry. A total of 855 proteins were identified among the two groups (0 d/15 d and 0 d/30 d). The Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses of differentially expressed proteins revealed that in ridge gourd seeds, the aging process altered the abundance of proteins related to the oxidative stress response, nutrient reservoir, and metabolism pathway. The most identified DEPs were mitochondrial proteins, ubiquitin−proteasome system proteins, ribosomal proteins, carbohydrate metabolism-related proteins, and stress response-related proteins. This study also presented the involvement of aconitase and glutathione pathway-associated enzymes in seed aging, with aconitase and total glutathione being determined as possible suggestive biomarkers for aged ridge gourd seeds. This acquired knowledge has the potential to considerably improve growing methods and seed preservation techniques, enhancing seed storage and maintenance.

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Evaluation of genetic integrity of tomato seeds during ageing by microsatellite markers

Cited by 7 publications

References 7 publications

First the seed: Genomic advances in seed science for improved crop productivity and food security

First the seed: Genomic advances in seed science for improved crop productivity and food security

Biology of Seed Vigor in the Light of -omics Tools

Comparative Proteomic Analysis of Ridge Gourd Seed (Luffa acutangula (L.) Roxb.) during Artificial Aging

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