“…Although a number of advanced marker systems, both dominant and codominant as well as high throughput marker systems have developed, ISSR and RAPD have still maintained its credibility in wheat diversity studies due to some specific features like reliability and reproducibility of ISSR and cost-effectiveness of RAPD. Several workers have advocated RAPD and ISSR markers to estimate the genetic divergence in hexaploid wheat from different regions of the world (Bhutta et al, 2006;Cao et al, 1998;Du et al, 2002;Freitas et al, 2000;Hao et al, 2006, Khan et al, 2010Khavarinejad and Karimov, 2012;Malik et al, 2008;Maric et al, 2004;Mukhtar et al, 2002;Najaphy et al, 2011;Rehman et al, 2013).…”
Section: Discussionmentioning
confidence: 99%
“…Malik et al (2008) studied the genetic diversity in bread wheat (Triticum aestivum L.) varieties released for high yield, quality and abiotic stress in India and found 68.42% polymorphism using 20 UBC series ISSR markers. Najaphy et al (2011) utilized 10 ISSR primers that generated 80.2% polymorphism among 30 wheat accessions. Genetic similarity data reported in this study confirmed the results from preceding researchers and can be further correlated with the agronomic and physiological responses of these varieties that may be supportive in the oblique assortment of efficient bread wheat genotypes (Liu et al, 2012).…”
Section: Discussionmentioning
confidence: 99%
“…High annealing temperature contributes significantly towards the elevated reproducibility among ISSR markers as compared to RAPD markers (Bornet and Branchard, 2001;Chowdhury et al, 2002). A number of researchers all over the world estimated genetic diversity/genetic similarity among bread wheat varieties using ISSR markers (Carvalho et al, 2009;Du et al, 2002;Hao et al, 2006;Khan et al, 2014;Najaphy et al, 2011;Rashed et al, 2008). 71 Hence, in present study, the efficacy of RAPD and ISSR markers has been explored in assessing the genetic structure in a collection of Indian and Turkish bread wheat varieties/cultivars and for the identification of genetic relations between the geographical groups.…”
Bread wheat (Triticum aestivum) is the most commonly grown crop due to its adaptation in a wide range of ecogeographical conditions and providing enhanced food assurance to the modern world. A diverse and rich collection is the foundation of each successful wheat improvement program. Therefore, major efforts are in progress worldwide to boost wheat production by broadening genetic diversity. Accepting this issue as a target, present study gives an overview of the major progress in the diversity and population evaluation of Indian and Turkish hexaploid wheat employing ISSR and RAPD primers. Various statistical analyses were employed for determining the hexaploid wheat population structure of India and Turkey. Results of dendrogram, scatterplots, Analysis of Molecular Variance (AMOVA) and population structure analysis were found in accordance with each other. All the experimental genotypes were clustered in two main groups, one group containing Indian varieties and another group containing both Indian and Turkish varieties reflecting the direct or indirect interbreeding among the populations of the two countries. Utilizing the genetic association of Indian and Turkish hexaploid wheat population, based on genetic distance estimated in the study, researchers worldwide may include Indian and Turkish hexaploid varieties in the wheat improvement programs and can evade the likelihood of selected germplasm becoming hereditarily consistent.
“…Although a number of advanced marker systems, both dominant and codominant as well as high throughput marker systems have developed, ISSR and RAPD have still maintained its credibility in wheat diversity studies due to some specific features like reliability and reproducibility of ISSR and cost-effectiveness of RAPD. Several workers have advocated RAPD and ISSR markers to estimate the genetic divergence in hexaploid wheat from different regions of the world (Bhutta et al, 2006;Cao et al, 1998;Du et al, 2002;Freitas et al, 2000;Hao et al, 2006, Khan et al, 2010Khavarinejad and Karimov, 2012;Malik et al, 2008;Maric et al, 2004;Mukhtar et al, 2002;Najaphy et al, 2011;Rehman et al, 2013).…”
Section: Discussionmentioning
confidence: 99%
“…Malik et al (2008) studied the genetic diversity in bread wheat (Triticum aestivum L.) varieties released for high yield, quality and abiotic stress in India and found 68.42% polymorphism using 20 UBC series ISSR markers. Najaphy et al (2011) utilized 10 ISSR primers that generated 80.2% polymorphism among 30 wheat accessions. Genetic similarity data reported in this study confirmed the results from preceding researchers and can be further correlated with the agronomic and physiological responses of these varieties that may be supportive in the oblique assortment of efficient bread wheat genotypes (Liu et al, 2012).…”
Section: Discussionmentioning
confidence: 99%
“…High annealing temperature contributes significantly towards the elevated reproducibility among ISSR markers as compared to RAPD markers (Bornet and Branchard, 2001;Chowdhury et al, 2002). A number of researchers all over the world estimated genetic diversity/genetic similarity among bread wheat varieties using ISSR markers (Carvalho et al, 2009;Du et al, 2002;Hao et al, 2006;Khan et al, 2014;Najaphy et al, 2011;Rashed et al, 2008). 71 Hence, in present study, the efficacy of RAPD and ISSR markers has been explored in assessing the genetic structure in a collection of Indian and Turkish bread wheat varieties/cultivars and for the identification of genetic relations between the geographical groups.…”
Bread wheat (Triticum aestivum) is the most commonly grown crop due to its adaptation in a wide range of ecogeographical conditions and providing enhanced food assurance to the modern world. A diverse and rich collection is the foundation of each successful wheat improvement program. Therefore, major efforts are in progress worldwide to boost wheat production by broadening genetic diversity. Accepting this issue as a target, present study gives an overview of the major progress in the diversity and population evaluation of Indian and Turkish hexaploid wheat employing ISSR and RAPD primers. Various statistical analyses were employed for determining the hexaploid wheat population structure of India and Turkey. Results of dendrogram, scatterplots, Analysis of Molecular Variance (AMOVA) and population structure analysis were found in accordance with each other. All the experimental genotypes were clustered in two main groups, one group containing Indian varieties and another group containing both Indian and Turkish varieties reflecting the direct or indirect interbreeding among the populations of the two countries. Utilizing the genetic association of Indian and Turkish hexaploid wheat population, based on genetic distance estimated in the study, researchers worldwide may include Indian and Turkish hexaploid varieties in the wheat improvement programs and can evade the likelihood of selected germplasm becoming hereditarily consistent.
“…RP value ranged from 1.13 to 33.00 with an average of 9.06 per primer. The highest RP was recorded by the primer 8 followed by the primer 18 referring to their importance in genetic diversity, because of RP positive correlation with the total amplified bands [14].…”
DNA-based molecular markers such as Inter Simple Sequence Repeat (ISSR), Sequence-Related Amplified polymorphism (SRAP) and Random Amplified Polymorphic DNA (RAPD) were used in this study to examine the genetic differences among sixteen Iraqi wheat varieties. Seventy three primers out of 177 were reproducible and showed clear amplified bands. The degree of genetic diversity, Polymorphism information content (PIC) and resolving power (RP) were estimated. All the studied molecular markers were informative and showed good ability to classify and distinguish 16 wheat varieties. Total number of polymorphic bands is 134, 221 and 55 for ISSR, SRAP and RAPD respectively. PIC and RP values were 0.259, 0.264 and 0.262 and 9.06, 7.87 and 2.78
Original Research ArticleThis document is published under a Creative Commons/Open Access license. Al-Kaab et al.; AJEA, 11(3): 1-11, 2016; Article no.AJEA.20371 2 respectively. It seems that SRAP marker was the most optimal technique for characterization the of Iraqi wheat varieties. The most efficient primers were Ne ISSR 7 for ISSR; Me4-Em3 and Me4-Em9 primers combination for SRAP; Operon primers K14, K15 and K19 for RAPD. The results of this study can be used to as starting point of the data base for Iraqi wheat varieties to be used in the future development of new varieties in a breeding programs.
“…The ISSR markers could be efficiently used to evaluate genetic variation in the wheat genotypes (Sofalian et al 2008;Najaphy et al 2011). Different molecular markers are currently available for genome mapping and tagging of different traits that is useful for marker assisted breeding techniques of wheat improvement under stress conditions (Ashraf 2010).…”
This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ABSTRACT The genetic variability and yield stability are complex traits with large environmental components that are utilized for breeding of improved plant performance under drought conditions. In order to better understand the relationship among genotype, trait components, and environment, marker-trait associations in 20 wheat varieties using phenotypic and ISSR (Inter-Simple Sequence Repeat) markers under drought conditions were examined. The combined analysis of variance results showed that the tested traits were significantly influenced by years, irrigation treatments, and the varieties. The drought stress conditions resulted in a substantial reduction in grain yield by 33.70%. The varieties viz., Shakha-93, Sahel-1, Giza-160, Giza-168, Sids-1, Sids-4 and Nilen were relatively drought resistant (DSI, Drought Susceptibility Index values< 1) and gave high grain yield when compared with the average of all varieties under drought stress. The stability analysis revealed that the cultivar named 'Sids-1' showed high and stable yield (bi=1 and S 2 di = 0), so it could be selected to be grown under drought stress conditions. On the other hand, Shakha-92 and Bacanora-88 showed below-average stability (bi = 1.
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