Background: The cotton crop is one of the most important natural fibers crops for textile manufacture in the world. The present research uses Pearson’s correlation coefficient and multivariate analysis to assess the interrelationships, similarities and dissimilarities among non- segregation (P1, P2 and F1) and segregation (F2, BC1 and BC2) generations for seed cotton yield and yield components in the two crosses Giza 92 x Pima S6 and Giza 93 x C.B. 58.Results: The analysis of variance exhibited highly significant genetic variability among six generations for all studied traits in the two crosses. The F1 performance was higher than the other generations for all the studied traits in the two crosses. The results had positive and high significant correlations between seed cotton yield/plant, lint cotton yield/plant and No. of bolls/plant traits across all six generations in the two crosses. A number of positive correlations were observed among the six generations for all studied traits in the two crosses. The UPGMA hierarchical clustering showed higher level of similarity coefficients among the six generations and among the studied traits. Similarity coefficients ranged from 0.96 to 0.99 and from 0.65 to 0.96, respectively. In the principal component analysis (PCA), the PCA1 extracted had Eigenvalue >1 across six generations for all studied traits in the two crosses. The PCA displayed total variation of 91.84% among the six generations contributed by PCA1 (79.47%) and PCA2 (12.38%) and had mainly distinguished the generations in different groups. The PCA1 and PCA2 were dominated by F1 and segregation generations in the two crosses, respectively, showing high correlations with the first two PCAs. All studied traits as well as boll weight and lint percentage traits contributed positive significant component loadings for the PCA1 and PCA2, respectively. The biplot analysis of the relationship between the six generations revealed that the most appropriate generations for selecting yield traits were F1 in the two crosses and BC1 and BC2 in the cross Giza 93 x C.B. 58. Conclusion: From the obtained results, we recommend considering backcrossing may be done for 2–5 cycles (BC2 – BC5) at C.B. 58 parent for improving Egyptian cotton yield in the future.