Evaluation of Gene Expression of TNF-α in Healthy Subjects

Abstract

Tumor necrosis factor-alpha (TNF-α) is a cytokine involved in the immune response, inflammation, and apoptosis. Dysregulation of TNF-α expression has been associated with various diseases, including autoimmune disorders, cancer, and chronic inflammatory conditions. Understanding the regulation of TNF-α expression in healthy individuals can help identify potential therapeutic targets for these diseases. Objective: To evaluate of gene expression of TNF-α in healthy subjects. Methods: The cross-sectional study conducted on 40 individuals on healthy individuals. RNA was extracted and TNF-α gene expression was evaluated using PCR and statistical analysis was done using SPSS software. Results: The evaluation of TNF-α gene expression in healthy individuals has also led to the identification of potential biomarkers of disease and new therapeutic targets. Conclusion: In conclusion, the evaluation of TNF-α gene expression in healthy individuals is an important tool for identifying potential biomarkers of disease and understanding the physiological role of this cytokine.