Evaluation of gene expression in pigs selected for enhanced reproduction using differential display PCR: II. Anterior pituitary1

Bertani, G.; Gladney, C. D.; Johnson, R. K.; Pomp, Daniel

doi:10.2527/2004.82132x

Cited by 15 publications

(14 citation statements)

References 34 publications

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“…Moreover, no additive and dominance effect was found. Although large differences in allele frequencies were found for genetic marker within BsuRI-c.930A>G-FSH locus, influences of potential genetic drift were confounded with those results because the additive and dominance effects of the allele did not differ from zero for any trait (Bertani et al, 2004). The results of HaeIII-g.5894A>G-FSH failed to confirm population wide linkage disequilibrium between the HaeIII-g.5894A>G-FSH polymorphism and litter size traits suggesting that the degree of linkage disequilibrium probably varies between populations (Gibson et al, 2002).…”

Section: Fsh Polymorphisms Discovery and Associationmentioning

confidence: 99%

“…Other candidate genes affected litter size traits were prolactin receptor (Drogemüller et al, 2001), retinol-binding protein4 (Linville et al, 2001), osteopontin (Short et al, 1997b), leukemia inhibitory factor (Spötter et al, 2005) folate binding protein (Valet et al, 2005a) and erythropoietin receptor (Vallet et al, 2005b). Bertani et al (2004) identified transcriptional candidate genes for fertility in anterior pituitary gland of pigs. FSH was found 10% highly expressed between sows selected for ovulation rate and embryo survival compared with the control line.…”

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confidence: 99%

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Novel BsuRI-c.930A>G-FSHb Associated with Litter Size Traits on Large White X Landrace Crossbred Sows

Pripwai¹,

Mekchay²

2011

JAS

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The objective of this experiment was to identify the novel single nucleotide polymorphisms (SNPs) on porcine follicle stimulating hormone  subunit (FSH) genes. Moreover, their association with litter size traits in commercial pigs will be analyzed. 1,155 Large White x Landrace crossbred sows were bled and DNA was extracted. The records included total number of piglet born (TNB), number of piglet born alive (NBA), number of piglets stillbirth (SB) and number of piglets mummified (MM). The known sequence of porcine FSH (GenBank accession no. D00621.1) was screened homology to known sequences in term of express sequenced tags (ESTs) in public domain, GenBank. The primers were designed for amplified the novel BsuRI-c.930A>G-FSH fragment which confirmed by PCR-RFLP and nucleotide sequencing then genotyping. The favorable cut homozygous G/G allele was highly significant higher than A/G allele in terms of TNB and NBA. While, published marker HaeIII-g.5894A>G-FSH was not significant difference in any litter traits. For haplotype analysis, c.930A>G-g.5894A>G of FSHβ, unfavorable GG/AA haplotype was significantly lower TNB and NBA than other haplotypes. Conversely, this haplotype was significantly higher MM than others. The study concluded that BsuRI-c.930A>G and the haplotype of HaeIII-g.5894A>G -BsuRI-c.930A>G of FSH may used for marker-assisted selection on pig breeding program.

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Section: Fsh Polymorphisms Discovery and Associationmentioning

confidence: 99%

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confidence: 99%

Novel BsuRI-c.930A>G-FSHb Associated with Litter Size Traits on Large White X Landrace Crossbred Sows

Pripwai¹,

Mekchay²

2011

JAS

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“…The study of these gene expression changes could be a means to gain insight into the genomic regulation of estrous behavior in cows. Gene expression studies are useful for discovering the biological principles that underlie polygenic traits (Bertani et al, 2004) like estrus. There is limited information on genes that regulate the reproductive behavior in dairy cows, but considerable knowledge is available from other species, especially the rodents.…”

Section: Introductionmentioning

confidence: 99%

Estrous behavior in dairy cows: identification of underlying mechanisms and gene functions

Bôer

Veerkamp

Beerda

et al. 2010

Animal

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Selection in dairy cattle for a higher milk yield has coincided with declined fertility. One of the factors is reduced expression of estrous behavior. Changes in systems that regulate the estrous behavior could be manifested by altered gene expression. This literature review describes the current knowledge on mechanisms and genes involved in the regulation of estrous behavior. The endocrinological regulation of the estrous cycle in dairy cows is well described. Estradiol (E2) is assumed to be the key regulator that synchronizes endocrine and behavioral events. Other pivotal hormones are, for example, progesterone, gonadotropin releasing hormone and insulin-like growth factor-1. Interactions between the latter and E2 may play a role in the unfavorable effects of milk yield-related metabolic stress on fertility in high milk-producing dairy cows. However, a clear understanding of how endocrine mechanisms are tied to estrous behavior in cows is only starting to emerge. Recent studies on gene expression and signaling pathways in rodents and other animals contribute to our understanding of genes and mechanisms involved in estrous behavior. Studies in rodents, for example, show that estrogen-induced gene expression in specific brain areas such as the hypothalamus play an important role. Through these estrogen-induced gene expressions, E2 alters the functioning of neuronal networks that underlie estrous behavior, by affecting dendritic connections between cells, receptor populations and neurotransmitter releases. To improve the understanding of complex biological networks, like estrus regulation, and to deal with the increasing amount of genomic information that becomes available, mathematical models can be helpful. Systems biology combines physiological and genomic data with mathematical modeling. Possible applications of systems biology approaches in the field of female fertility and estrous behavior are discussed.

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“…SNPs within this gene were examined for effects on these traits in commercial sows. FTH was chosen based on their biological functions to be evaluated for association between polymorphisms and litter traits, because of differentially expressed gene in the anterior pituitary gland of sows selected of long-term for increased ovulation rate and embryo survival that was confirmed by using Northern hybridization (Bertani et al, 2004), and the ferroxidase activity of the ferritin molecule which could transform the more toxic Fe(II) into the less toxic Fe(III) that was essential for detoxification of iron and/or for maintaining its bioavailability during embryonic development (Ferreira et al, 2000). These functions seemed the most likely to have an effect on the fetuses development.…”

Section: Fth Polymorphisms Discovery and Associationmentioning

confidence: 99%

“…Putative QTL regions are located on SSC6 (Wilkie et al, 1999), SSC7, SSC12, SSC14 and SSC17 (de Koning et al, 2001). Bertani et al (2004) identified transcriptional candidate genes for fertility in anterior pituitary gland of pigs. Ferritin heavy chain (FTH) was found highly expressed between sows selected for ovulation rate and embryo survival compared with the control line, 42% and 10%, respectively.…”

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confidence: 99%

Haplotype Analysis of Ferritin Heavy Chain Affects Stillborn Piglets on Large White X Landrace Crossbred Sows

Pripwai¹,

Mekchay²

2011

JAS

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The objective of this experiment was to identify the novel SNP on porcine FTH genes. Moreover, their association with litter size traits in commercial pigs will be analyzed. 1,361 Large White x Landrace crossbred sows were bled and DNA was extracted. The records included total number of piglets born (TNB), number of piglets born alive (NBA), number of piglets stillborn (SB) and number of piglets mummified (MM). The PCR primers were designed throughout the porcine FTH . PCR products were performed and screened for single nucleotide polymorphisms (SNPs) by using polymerase chain reaction -single strand conformation polymorphism (PCR-SSCP). Consequently, differentially heteroduplex shift were cloned and sequenced. SNPs including c.596C>T, c.634A>G c.677T>C and c.699T>C were found. According to these SNPs, there were proved by PCR-RFLP technique, BsuRI-c. 596C>T,. Six different haplotypes for the SNPs of MspI at nucleotide position were presented. The TCTC haplotype was significantly associated with increasing SB. It was conclude that MspI-FTH haplotype significantly associated with SB in commercial pigs which may used for marker-assisted selection on pig breeding program.

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Evaluation of gene expression in pigs selected for enhanced reproduction using differential display PCR: II. Anterior pituitary1

Cited by 15 publications

References 34 publications

Novel BsuRI-c.930A>G-FSHb Associated with Litter Size Traits on Large White X Landrace Crossbred Sows

Novel BsuRI-c.930A>G-FSHb Associated with Litter Size Traits on Large White X Landrace Crossbred Sows

Estrous behavior in dairy cows: identification of underlying mechanisms and gene functions

Haplotype Analysis of Ferritin Heavy Chain Affects Stillborn Piglets on Large White X Landrace Crossbred Sows

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