Evaluation of Functional Recovery in Rats After Median Nerve Resection and Autograft Repair Using Computerized Gait Analysis

Heinzel, Johannes; Oberhauser, Viola; Keibl, Claudia; Swiadek, Nicole Victoria; Längle, Gregor; Frick, Helen; Kolbenschlag, Jonas; Prahm, Cosima; Grillari, Johannes; Hercher, David

doi:10.3389/fnins.2020.593545

Cited by 17 publications

(42 citation statements)

References 81 publications

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“…Gait analysis was performed using the CATWALK XT system (AsterWee Information Technology), which records and analyzes the voluntary movements of rats on a closed walkway and has proven to be a reliable method for measuring pain-related behavior [ 41 – 43 ]. In brief, green light was reflected from the inside onto a glass panel that held an enclosed corridor with a red backlight above it.…”

Section: Methodsmentioning

confidence: 99%

Rosiglitazone Alleviates Mechanical Allodynia of Rats with Bone Cancer Pain through the Activation of PPAR-γ to Inhibit the NF-κB/NLRP3 Inflammatory Axis in Spinal Cord Neurons

Zhao

et al. 2021

PPAR Research

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Bone cancer pain (BCP) is a serious clinical problem that affects the quality of life of cancer patients. However, the current treatment methods for this condition are still unsatisfactory. This study investigated whether intrathecal injection of rosiglitazone modulates the noxious behaviors associated with BCP, and the possible mechanisms related to this effect were explored. We found that rosiglitazone treatment relieved bone cancer-induced mechanical hyperalgesia in a dose-dependent manner, promoted the expression of peroxisome proliferator-activated receptor-γ (PPAR-γ) in spinal cord neurons, and inhibited the activation of the nuclear factor-kappa B (NF-κB)/nod-like receptor protein 3 (NLRP3) inflammatory axis induced by BCP. However, concurrent administration of the PPAR-γ antagonist GW9662 reversed these effects. The results show that rosiglitazone inhibits the NF-κB/NLRP3 inflammation axis by activating PPAR-γ in spinal neurons, thereby alleviating BCP. Therefore, the PPAR-γ/NF-κB/NLRP3 signaling pathway may be a potential target for the treatment of BCP in the future.

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Section: Methodsmentioning

confidence: 99%

Rosiglitazone Alleviates Mechanical Allodynia of Rats with Bone Cancer Pain through the Activation of PPAR-γ to Inhibit the NF-κB/NLRP3 Inflammatory Axis in Spinal Cord Neurons

Zhao

et al. 2021

PPAR Research

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“…[ [13][14][15][16] Stand duration Stand time of the paw; stand time/stance phase (sec). [17,18] Swing duration Duration of the step cycle phase; swing time/swing phase. [13][14][15][16][17][18] Step cycle duration Duration of the step cycle (stance and swing duration) (sec).…”

Section: Dynamic Paw Parametersmentioning

confidence: 99%

“…[17,18] Swing duration Duration of the step cycle phase; swing time/swing phase. [13][14][15][16][17][18] Step cycle duration Duration of the step cycle (stance and swing duration) (sec). [14] Duty cycle (%) Stand time divided by stand time + swing time.…”

Section: Dynamic Paw Parametersmentioning

confidence: 99%

“…The distance between the two forelimbs or between the two hind limbs. [13,14,[16][17][18] Stride length Length (mm) of a stride; distance between two consecutive placements of a paw.…”

Section: Static Paw Parametersmentioning

confidence: 99%

“…[17,19] Mean paw print intensity Mean intensity of the paw. [13,[16][17][18][19] Maximum intensity at maximum contact Maximum intensity of the paw at maximum contact. [17,19] Relative paw position Relative positions of fore and hind paws: hind paw position is related to the previous forepaw position.…”

Section: Static Paw Parametersmentioning

confidence: 99%

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Gait Analysis Using Animal Models of Peripheral Nerve and Spinal Cord Injuries

2021

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The present review discusses recent data regarding rodent models of spinal cord and peripheral nerve injuries in terms of gait analysis using the CatWalk system (CW), an automated and exceptionally reliable system for assessing gait abnormalities and motor coordination. CW is a good tool for both studying improvements in the walking of animals after suffering a peripheral nerve and spinal cord lesion and to select the best therapies and procedures after tissue destruction, given that it provides objective and quantifiable data. Most studies using CW for gait analysis that were published in recent years focus on injuries inflicted in the peripheral nerve, spinal cord, and brain. CW has been used in the assessment of rodent motor function through high-resolution videos, whereby specialized software was used to measure several aspects of the animal’s gait, and the main characteristics of the automated system are presented here. CW was developed to assess footfall and gait changes, and it can calculate many parameters based on footprints and time. However, given the multitude of parameters, it is necessary to evaluate which are the most important under the employed experimental circumstances. By selecting appropriate animal models and evaluating peripheral nerve and spinal cord lesion regeneration using standardized methods, suggestions for new therapies can be provided, which represents the translation of this methodology into clinical application.

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Targeted Repair of Spinal Cord Injury Based on miRNA‐124‐3p–Loaded Mesoporous Silica Camouflaged by Stem Cell Membrane Modified with Rabies Virus Glycoprotein

Fan,

Shi,

Yang

et al. 2024

Advanced Science

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Spinal cord injury (SCI) has no effective treatment modalities. It faces a significant global therapeutical challenge, given its features of poor axon regeneration, progressive local inflammation, and inefficient systemic drug delivery due to the blood–spinal cord barrier (BSCB). To address these challenges, a new nano complex that achieves targeted drug delivery to the damaged spinal cord is proposed, which contains a mesoporous silica nanoparticle core loaded with microRNA and a cloaking layer of human umbilical cord mesenchymal stem cell membrane modified with rabies virus glycoprotein (RVG). The nano complex more readily crosses the damaged BSCB with its exosome‐resembling properties, including appropriate size and a low‐immunogenic cell membrane disguise and accumulates in the injury center because of RVG, where it releases abundant microRNAs to elicit axon sprouting and rehabilitate the inflammatory microenvironment. Culturing with nano complexes promotes axonal growth in neurons and M2 polarization in microglia. Furthermore, it showed that SCI mice treated with this nano complex by tail vein injection display significant improvement in axon regrowth, microenvironment regulation, and functional restoration. The efficacy and biocompatibility of the targeted delivery of microRNA by nano complexes demonstrate their immense potential as a noninvasive treatment for SCI.

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Evaluation of Functional Recovery in Rats After Median Nerve Resection and Autograft Repair Using Computerized Gait Analysis

Cited by 17 publications

References 81 publications

Rosiglitazone Alleviates Mechanical Allodynia of Rats with Bone Cancer Pain through the Activation of PPAR-γ to Inhibit the NF-κB/NLRP3 Inflammatory Axis in Spinal Cord Neurons

Rosiglitazone Alleviates Mechanical Allodynia of Rats with Bone Cancer Pain through the Activation of PPAR-γ to Inhibit the NF-κB/NLRP3 Inflammatory Axis in Spinal Cord Neurons

Gait Analysis Using Animal Models of Peripheral Nerve and Spinal Cord Injuries

Targeted Repair of Spinal Cord Injury Based on miRNA‐124‐3p–Loaded Mesoporous Silica Camouflaged by Stem Cell Membrane Modified with Rabies Virus Glycoprotein

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