Evaluation of four serological tests for the diagnosis of caprine melioidosis

Abstract

A complement fixation (CF) test, 2 indirect haemagglutination (IHA-A; IHA-L) tests which differed in antigen preparation and technique, and a microtitre agglutination (MA) test were compared in the serodiagnosis of melioidosis in goats. One hundred and eighteen experimental serums and 3143 field serums from goats in endemic and non-endemic areas of north Queensland were used in the evaluation. Culture of samples for Pseudomonas pseudomallei from 112 goats provided substantiating evidence of infection. The IHA-A test was the most sensitive, and the CF test the most specific. We advocate the use of the IHA-A as a screening test followed by the CF test for confirmation of active melioidosis. The IHA-A test is the better indicator of past infection.

Melioidosis: Epidemiology, Pathophysiology, and Management

“…A wide variety of animal species have been shown to be susceptible to melioidosis, including camels, horses, sheep, cattle, goats, pigs, and kangaroos (156,237,252,426,429); koalas (253); alpacas (220a); deers, cats, and dogs (277); and captive marine animals (193). Cattle, water buffalo, and crocodiles are considered to be relatively resistant to melioidosis despite their constant exposure to mud (53,277).…”

Case-control investigation on the risk factors of melioidosis in small ruminant farms in Peninsular Malaysia

“…The CFT was the preferred as screening test because of its high specificity in serodiagnosis of melioidosis (Thomas et al . ). The sensitivity of the CFT in for diagnosis of melioidosis in animals was estimated to be between 79·3 and 82·4% while its specificity was between 99·5 and 100%, which qualifies the test to be a good screening test (Thomas et al .…”

Development and Clinical Evaluation of A PCR Assay Targeting the Metalloprotease Gene (mprA) of B. pseudomallei

“…Consequently, cases in animals have not only been reported from Australia, Papua New Guinea, Malaysia, Thailand, Viet Nam, Singapore and Taiwan, but also from China, the Indian subcontinent, Turkey, Iran, Saudi Arabia, United Arab Emirates, Madagascar, Egypt, Chad, Niger, South Africa, Aruba, USA, Brazil, UK, France and Spain (Sprague and Neubauer, 2004). Occasional infection in a multitude of warm‐ and cold‐blooded animals can occur by inhalation, ingestion, via skin wounds in contact with contaminated water or dust particles (Thomas et al., 1988). The clinical picture can present itself in various forms: acute fulminate septicaemia, local infection, subacute illness, chronic infection and subclinical disease.…”