Evaluation of four methods for rapid detection of adenovirus

Abstract

Four methods for rapid detection of adenovirus were evaluated by testing retrospectively 28 frozen clinical specimens from which an adenovirus strain had been isolated. After thawing all specimens were retested for the presence of adenovirus by conventional culture on KB cells and found to be positive. The four tests used for rapid detection of adenovirus were a 48-hour culture technique, and an immunoassay, a latex agglutination test and an immunofluorescence assay for direct detection of viral antigen using commercially available reagents. Of the 28 specimens all were positive in the 48-hour culture, 25 (89%) positive in the immunoassay and 10 (36%) positive in the latex agglutination test. Six of eight nasopharyngeal aspirate specimens were positive in the immunofluorescence assay. Twenty-five clinical specimens negative for adenovirus on conventional culture were also negative in the 48-hour culture technique. Overall, the rapid (48-hour) culture technique was 100% sensitive and 100% specific compared to conventional culture. The direct detection of viral antigen by immunoassay was less sensitive, however results were available within a few hours. Prospective comparative studies are warranted to determine whether these rapid techniques could replace conventional culture in the routine diagnosis of adenovirus infection.

Persistent Viral Infection in Primary Sjogren’s Syndrome: Review and Perspectives

“…This antibody recognizes an epitope in the Nterminal of capsid protein VP1 that is conserved in the enterovirus genus. Its specificity and sensitivity for the detection of enteroviruses in epithelial cell culture and tissue samples have been the subject of prior reports [27,28]. In our study, positive staining with the anti-VP1 antibody was observed in 11 out of 12 pSS samples, 1 out of 8 sSS samples, and 0 out of 16 controls.…”

Comparison of a rapid culture method combining an immunoperoxidase test and a group specific anti-VP1 monoclonal antibody with conventional virus isolation techniques for routine detection of enteroviruses in stools

“…Techniques using spin amplification and identification by a specific monoclonal antibody have been shown to shorten significantly the detection time of many viruses in cell culture, including cytomegalovirus [Agha et al, 1988;Alpert et al, 1985;Gleaves et al, 1984;Pozzetto et al, 1990], adenovirus [August and Warford, 1987;Trabelsi et al, 1992] sensitivity and specificity. In this study, the combined effect of sample centrifugation and immunological staining is probably responsible for the dramatic increase in sensitivity of RCA compared to CCA in the first three days of virus replication, as exemplified by the data in Table IV.…”

“…This disadvantage could be avoided by the combination of two RCA on the same samples using enterovirus and adenovirus group-specific monoclonal antibodies; in fact, we have already shown that a similar technique can be applied to adenoviruses [Trabelsi et al, 1992]. Further such studies are under development in our laboratory.…”

Haemorrhagic cystitis associated with adenovirus in a patient with AIDS treated for a non-Hodgkin's lymphoma

“…The rapid 48-h culture technique was performed by using 96-well plate centrifugation and immunoperoxydase staining with adenovirus monoclonal antibody (H-60 clone, Argene, France) as previously reported [3]. Conventional cell culture plates were observed periodically for cytopathic effects.…”