Evaluation of four Candida utilis strains for biomass, acetic acid and ethyl acetate production from ethanol

Christen, Pierre; Domenech, F.; Páca, Jan; Revah, Sergio

doi:10.1016/s0960-8524(98)00142-4

Cited by 20 publications

(12 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Potent producers of ethyl acetate are Pichia anomala [16][17][18][19][20][21], Candida utilis [22][23][24][25] and Kluyceromyces marxianus [26][27][28] (Candida pseudotropicalis and K. fragilis are considered to be synonyms of the latter). Ethyl acetate was formed from ethanol [17,18,[21][22][23][24]28], glucose [16, 18-20, 22, 25, 28], and whey-borne lactose alone or combined with ethanol [26,27].…”

Section: Introductionmentioning

confidence: 99%

“…Ethyl acetate was formed from ethanol [17,18,[21][22][23][24]28], glucose [16, 18-20, 22, 25, 28], and whey-borne lactose alone or combined with ethanol [26,27]. Often, the sugar is at first fermented to ethanol which thereafter is reacted to ethyl acetate [18,22,25,26,28].…”

Section: Introductionmentioning

confidence: 99%

“…One opportunity to handle the high volatility of ethyl acetate is the cultivation of yeasts in sealed shake flasks [16,17,22,25]. But often, aerobic cultivation processes were performed in shake flasks without mentioning or discussing the volatility of the formed ethyl acetate at all [18,19,23,28]. Even in reliably sealed bottles, some troubles as oxygen limitation and inhibition by accumulating ester may emerge.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Formation of ethyl acetate by Kluyveromyces marxianus on whey: studies of the ester stripping

Urit

Löser

Wunderlich

et al. 2010

Bioprocess Biosyst Eng

141

View full text Add to dashboard Cite

Kluyveromyces marxianus is capable of converting lactose into ethyl acetate offering a chance for an economical reuse of whey. The microbial formation of ethyl acetate as a bulk product calls for an aerobic process and, thus, the highly volatile ethyl acetate is discharged from the aerated bioreactor. This stripping process was modeled and investigated experimentally. The stripping rate was proportional to the gas flow and nearly independent of the stirring rate since the stripping was governed by the absorption capacity of the exhaust gas rather than the phase transfer. Cooling the exhaust gas did not noticeably influence the stripping. One batch experiment is presented in detail to demonstrate the formation of ethyl acetate by K. maxianus DSM 5422 on whey. Further batch experiments showed that a substantial formation of ethyl acetate only occurred when the yeast growth was limited by a lack of trace elements. The highest product yield observed was 0.25 g ethyl acetate per g lactose which is nearly 50% of the theoretical maximum.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Formation of ethyl acetate by Kluyveromyces marxianus on whey: studies of the ester stripping

Urit

Löser

Wunderlich

et al. 2010

Bioprocess Biosyst Eng

141

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

“…There are only a few publications dealing with the screening of yeasts regarding the formation of ethyl acetate and other volatile compounds [23,[27][28][29]. The described screening experiments were focused on ethyl acetate as an aroma compound formed under anaerobic conditions [23,29] or on ethyl acetate as a bulk product formed under aerobic conditions [27,28]. The yeasts were screened in bioreactors [23,29], which is time and labor-consuming, or the yeasts were tested in shake flasks without considering the ester stripping at all [27,28].…”

Section: Introductionmentioning

confidence: 99%

Screening of Kluyveromyces strains for the production of ethyl acetate: Design and evaluation of a cultivation system

Löser

Urit

Nehl

et al. 2011

Engineering in Life Sciences

View full text Add to dashboard Cite

Some members of the yeast Kluyveromyces marxianus are known to convert sugars and ethanol into ethyl acetate. This metabolic activity offers a chance for an economically favorable use of whey. An exemplary batch experiment is presented to demonstrate the formation of ethyl acetate by K. marxianus DSM 5422 on whey‐based medium in an aerated 70‐L stirred reactor. A screening system was developed to check Kluyveromyces strains regarding their capability to form ethyl acetate. The designed screening system takes the volatility of ethyl acetate and the requirement of oxygen for sugar conversion into account and allows the analysis of ester formation kinetics. Modeling and measurement of the ester stripping from screening bottles guaranteed reliable data evaluation. Twelve of 23 tested strains produced noteworthy amounts of ethyl acetate with a yield between 0.13 and 0.23 g ester per gram sugar consumed. The productive strains showed individual kinetics of ester formation; some strains had a short interval of intense production while others exhibited a longer period of stable formation. Another factor of interest was the selectivity of product formation expressed as the mass ratio of formed ester and ethanol, which ranged from 0.9 to 3.5 g/g.

show abstract

Synthesis of ethyl acetate from glucose by Kluyveromyces marxianus, Cyberlindnera jadinii and Wickerhamomyces anomalus depending on the induction mode

Hoffmann

Kupsch

Walther

et al. 2020

Engineering in Life Sciences

View full text Add to dashboard Cite

Ethyl acetate is currently produced from fossil carbon resources. This ester could also be microbially synthesized from sugar‐rich wastes of the food industry. Wild‐type strains with GRAS status are preferred for such applications. Production of ethyl acetate by wild‐type yeasts has been repeatedly reported, but comparative studies with several strains at various induction modes are largely missing. Here, synthesis of ethyl acetate by three yeasts with GRAS status, Kluyveromyces marxianus DSM 5422, Cyberlindnera jadinii DSM 2361 and Wickerhamomyces anomalus DSM 6766, was studied under identical and well‐defined conditions in an aerated bioreactor, by inducing the ester synthesis via iron or oxygen limitation. Balancing the ester synthesis was based on measured concentrations of ethyl acetate in the exhaust gas, delivering masses of synthesized ester and synthesis rates in a high temporal resolution. All tested yeasts synthesized ethyl acetate under these conditions, but the intensity varied with the strain and induction mode. The highest yields were achieved under iron limitation with K. marxianus (0.182 g g–1) and under oxygen limitation with W. anomalus (0.053 g g–1). Iron limitation proved to be the better inducer for ester synthesis while oxygen limitation favored ethanol formation. K. marxianus DSM 5422 was the most potent producer of ethyl acetate exhibiting the highest biomass‐specific synthesis rate of 0.5 g g–1h–1 under moderate iron limitation.

show abstract

Evaluation of four Candida utilis strains for biomass, acetic acid and ethyl acetate production from ethanol

Cited by 20 publications

References 6 publications

Formation of ethyl acetate by Kluyveromyces marxianus on whey: studies of the ester stripping

Formation of ethyl acetate by Kluyveromyces marxianus on whey: studies of the ester stripping

Screening of Kluyveromyces strains for the production of ethyl acetate: Design and evaluation of a cultivation system

Synthesis of ethyl acetate from glucose by Kluyveromyces marxianus, Cyberlindnera jadinii and Wickerhamomyces anomalus depending on the induction mode

Contact Info

Product

Resources

About