Evaluation of Forages in the Laboratory. I. Comparative Accuracy of Several Methods

Baumgardt, B.R.; Cason, J.L.; Taylor, M. W.

doi:10.3168/jds.s0022-0302(62)89327-8

Cited by 18 publications

(4 citation statements)

References 7 publications

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“…Either 50 or 125pCi [8-14C]adenine were dissolved in 2 ml 0.02 M-sodium hydroxide and added to a growing bacterial culture under anaerobic conditions. The culture contained 27 ml clarified rumen fluid and 67 ml buffer (Baumgardt et al 1962). Optimum growth of bacteria was obtained within 24 h. The whole culture was transferred to tubes and centrifuged at 40000g for 20 min.…”

mentioning

confidence: 99%

Metabolism of the nucleic acids of rumen bacteria by preruminant and ruminant lambs

Razzaque

Topps

Kay³

et al. 1981

Br J Nutr

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1.A rumen bacterial culture containing specifically labelled nucleic acids was prepared using [S-"C]adenine. 2. The labelled preparation was given in a liquid diet to two preruminant lambs and via a rumen tube to two ruminant lambs. The radioactivity excreted in exhaled gases, faeces and urine and that incorporated into tissues was determined.3 The preruminant lambs absorbed 58.3% of the total radioactivity measured after 24 h and the ruminant lambs 66.6% of the total activity measured after 48 h.4. Of the total radioactivity absorbed the preruminant lambs exhaled 38%, excreted 34% in urine and.retained 29% in tissues. The corresponding values for the ruminant lambs were 12,41 and 47% respectively.5. There was a close relationship between total nucleic acid content and radioactivity per g of tissues of both preruminant and ruminant lambs.6. Of the radioactivity in the urine, the ruminant and one preruminant lamb excreted most in the fraction containing allantoin, while the other lamb excreted most activity in the uric acid fraction. 7.The salvaging of the breakdown products of bacterial nucleic acids to make tissue nucleic acids appears to be an important synthesis in preruminant and ruminant lambs and of the likely precursors the purine base may be more important than the nucleoside. Ellis & Bleichner (1969) were the first to suggest that some of the purines are used for synthesis of nucleic acids in the tissues when they found that a large fraction of those absorbed were not immediately excreted in the urine. Unlike monogastric animals, ruminants appear to make little or no use of glycine, a normal precursor of purines, for nucleic acid synthesis (Condon et al. 1970).Smith et al. (1974) attempted to measure the utilization of the nucleic acids of rumen bacteria by sheep and their results indicate that at least 5% was incorporated into the liver, spleen and kidney and another 20% at least may appear in muscle. The present experiment has been conducted to study further the absorption, tissue deposition and excretion of purines derived from bacterial nucleic acids in ruminant lambs and compare the values obtained with those in preruminant lambs.

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confidence: 99%

Metabolism of the nucleic acids of rumen bacteria by preruminant and ruminant lambs

Razzaque

Topps

Kay³

et al. 1981

Br J Nutr

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“…Filtration was used to separate liquid and solid phases (Alexander and McGowan 1961) following the pepsin-HCl digestion. The artificial saliva solution used was a modification (Baumgardt et al 1962) of that described by McDougall (1947).…”

Section: Experiments Imentioning

confidence: 99%

Effect of Mefluidide on Yield and Chemical Composition of Crested Wheatgrass in East-Central Saskatchewan

McCaughey¹,

Cohen²

1990

Can. J. Plant Sci.

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“…Samples were incubated with rumen fluid for each treatment (with or without active yeast), plus McDougall buffer solution, in a glass tube, for 48 hours, in water bath at 39°C, according to the methodology described by [9] and based on the works by [10] and [11].…”

Section: Methodsmentioning

confidence: 99%

Active yeast (Saccharomyces cerevisiae) supplementation improves digestibility of lamb diet¹

Bueno¹

2013

IOSR-JAVS

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Evaluation of Forages in the Laboratory. I. Comparative Accuracy of Several Methods

Cited by 18 publications

References 7 publications

Metabolism of the nucleic acids of rumen bacteria by preruminant and ruminant lambs

Metabolism of the nucleic acids of rumen bacteria by preruminant and ruminant lambs

Effect of Mefluidide on Yield and Chemical Composition of Crested Wheatgrass in East-Central Saskatchewan

Active yeast (Saccharomyces cerevisiae) supplementation improves digestibility of lamb diet¹

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