Evaluation of flavonoids fromBauhinia megalandra leaves as inhibitors of glucose-6-phosphatase system

Estrada, Omar; Hasegawa, Masahisa; González-Mujica, Freddy; Motta, Norma; Perdomo, Elsa; Solorzano, Alejandra; Méndez, Jeannette; Méndez, Bernardo; Zea, Elsa

doi:10.1002/ptr.1703

Cited by 43 publications

(26 citation statements)

References 16 publications

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“…Enzymes, like glucokinase, involved in glycolysis and those, like glycogen synthase, implicated in glycogen synthesis decrease while gluconeogenic enzymes, like PEPCK and G-6-Pase, increase, leading to increased hepatic output in both type 1 as well as type 2 diabetes. Vanadium compounds have been reported to normalize the levels of these enzymes [24], while quercetin is known to have no or very little effect on these enzymes levels [25,26]. Since BQOV is a complex of vanadium and quercetin we thought it worthwhile to investigate which of these two components plays a role in restoring carbohydrate metabolism.…”

Section: Discussionmentioning

confidence: 99%

Bis(quercetinato)oxovanadium IV Reverses Metabolic Changes in Streptozotocin-Induced Diabetic Mice

Shukla

Padhyé²,

Modak³

et al. 2007

Rev Diabet Stud

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■ AbstractOrganic vanadium compounds offer several advantages in the treatment of diabetes, yet they are impractical to use because of known side effects. In order to ameliorate the side effects of vanadium, we conjugated it with quercetin to form bis(quercetinato)oxovanadium IV (BQOV). This study evaluates the effect of BQOV treatment on carbohydrate metabolism and overall oxidative stress in streptozotocininduced (STZ) diabetic mice. Administration of BQOV orally to diabetic mice for 3 weeks led to a reduction of blood glucose levels and the animals exhibited normal glucose tolerance at the end of the study period. The increase in glucose uptake by skeletal muscle and liver as well as the normalization of mRNA levels of G-6-Pase and glucokinase in the liver after BQOV treatment pointed to improvements in carbohydrate metabolism. The analysis of the antioxidant status of serum, liver and pancreas revealed reduced oxidative stress in BQOV-treated animals compared to untreated diabetic controls. Serum analyses for kidney and liver function showed that BQOV treatment provoked total protection of the kidney and partial protection of the liver from diabetogenic insults. The number of insulin-positive cells and the amount of pancreatic insulin in treated mice (1.2038 ± 0.34 ng/mg tissue) did not account for pancreatic regeneration but suggested an insulin-mimetic action on the part of BQOV. Moreover, administration of BQOV for 3 weeks did not show any visible side-effects. This data indicate that BQOV is a safe and potent agent for diabetes treatment, because it is able to improve carbohydrate metabolism and to reduce overall oxidative stress.

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Section: Discussionmentioning

confidence: 99%

Bis(quercetinato)oxovanadium IV Reverses Metabolic Changes in Streptozotocin-Induced Diabetic Mice

Shukla

Padhyé²,

Modak³

et al. 2007

Rev Diabet Stud

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“…Também atuam por inibição da glicose-6-fosfatase (G-6-Pase) (ESTRADA et al, 2005) que é um sistema enzimático do retículo endoplasmático responsável pela hidrólise da glicose-6-fosfato e a consequente formação de glicose nos órgãos que a possuem (fígado, rim e intestino) (SANTOS-ANTUNES, FONTES, 2009). Quando a enzima é inibida, observa-se redução da glicose.…”

Section: Tabela 1-ensaios In Vivo E In Vitro Realizados Com Plantas Dunclassified

Experimental Studies With Species of Bauhinia (Fabaceae) to Evaluate the Antidiabetic Activity

Paula

2016

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RESUMO:Modelos de estudo in vivo e in vitro e em enzimas/receptores são utilizados quando se pretende comprovar a ação farmacológica de um determinado extrato e/ou princípio ativo obtido de plantas, além de elucidar o mecanismo de ação. O objetivo deste estudo foi realizar um levantamento da literatura sobre quais os ensaios que já foram realizados com plantas do gênero Bauhinia L. para verificar a atividade hipoglicemiante, e quais as espécies pesquisadas. Para isso, foi realizado uma revisão da literatura através de busca eletrônica nas bases de dados Pubmed, Science direct, Lilacs e na biblioteca eletrônica Scielo, publicados no período de 1995 à 2015 (20 anos), utilizando como descritores "Bauhinia" and "extract" and "hypoglycemic" or " seus correspondentes em português, "Bauhinia" e "extrato" e antidiabetic" "hipoglicêmica" ou "antidiabético". Foram localizados artigos relacionados à pesquisa com doze espécies de Bauhinia L. A Bauhinia forficata é a que mais apresenta estudos sobre o tema, sendo localizado 13 artigos, seguida da B. variegata com 4 estudos. Foi possível verificar que pouco se conhece sobre a atividade hipoglicemiantes das espécies de Bauhinia L., e que mais estudos são necessários para comprovar o uso popular. Palavras-chave: pata de vaca, planta medicinal, diabetes ABSTRACT:In vivo and in vitro and in enzymes / receptors study models are used when you want to prove the pharmacological action of a particular extract and / or active ingredient from plants, as well as elucidate the mechanism of action. The aim of this study was to survey the literature on which trials have already been conducted with the genus Bauhinia L. plans to check the hypoglycemic activity, and what the surveyed species. For this, we conducted a literature review through electronic search on Pubmed, Science direct, Lilacs and electronic library SciELO, published from 1995 to 2015 (20 years), using as keywords "Bauhinia" and "extract "and" hypoglycemic "or" Antidiabetic "their equivalents in Portuguese," Bauhinia "and" extrato "and" hipoglicêmico "or" anti-diabético". Research-related articles were found with twelve species of Bauhinia L. The Bauhinia forficata is the one that presents studies on the subject, being located 13 articles, followed by B. variegata , 4 studies. It observed that little is known about the hypoglycemic activity of the species of Bauhinia L., and that further studies are needed to confirm the popular use.

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“…Inicialmente medimos los efectos del extracto acuoso de las hojas de B. megalandra (9) sobre la neoglucogénesis de rebanadas de hígado de rata, incubadas en Krebs Ringer bicarbonato suplementado con albúmina bovina saturada con oleato y utilizando lactato o fructosa como substrato neoglucogénico. Como se puede observar en la Tabla 1, la actividad neoglucogénica de las rebanadas controles es lineal hasta los 90 min.…”

Section: Efectos De B Megalandra Sobre La Neoglucogénesis Y La G-6-pasaunclassified

“…Como se muestra en la Tabla 2, cuando se usó G-6-P como substrato de la G-6-Pasa de microsomas intactos, el extracto foliar disminuyó de manera estadísticamente significativa la V MAX con una moderada elevación del K M sin afectar la actividad de la enzima de microsomas rotos (9) .…”

Section: Tablaunclassified

“…El extracto de la planta, no tuvo efecto alguno cuando el substrato de la enzima fue PPi, tanto en microsomas intactos como rotos; lo cual sugiere que el efecto del extracto de B. megalandra se ejerce sobre uno de los transportadores del sistema de la G-6-Pasa probablemente sobre T1 (9) . Posiblemente la inhibición de la neoglucogénesis hepática sea el resultado de la inhibición de la G-6-Pasa por los compuestos presentes en el extracto de la planta.…”

Section: Tablaunclassified

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Antihiperglycaemic Activity of Bauhinia megalandra

González-Mujica¹

2011

Phytochemicals - Bioactivities and Impact on Health

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Evaluation of flavonoids fromBauhinia megalandra leaves as inhibitors of glucose-6-phosphatase system

Cited by 43 publications

References 16 publications

Bis(quercetinato)oxovanadium IV Reverses Metabolic Changes in Streptozotocin-Induced Diabetic Mice

Bis(quercetinato)oxovanadium IV Reverses Metabolic Changes in Streptozotocin-Induced Diabetic Mice

Experimental Studies With Species of Bauhinia (Fabaceae) to Evaluate the Antidiabetic Activity

Antihiperglycaemic Activity of Bauhinia megalandra

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