Evaluation of five immunoturbidimetric assays for urinary albumin quantification and their impact on albuminuria categorization

Bargnoux, Anne-Sophie; Barrot, Amandine; Fesler, Pierre; Kuster, Nils; Badiou, Stéphanie; Dupuy, Anne‐Marie; Ribstein, Jean; Cristol, Jean‐Paul

doi:10.1016/j.clinbiochem.2014.07.014

Cited by 12 publications

(7 citation statements)

References 13 publications

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“…There is a semiquantitative point‐of‐care ELISA test and three assays that require more complex laboratory analyzers, including the ELISA, immunoturbidimetry, and high‐resolution electrophoresis. Immunoturbidimetry is a frequently used method in human medicine 20 that was validated for dogs in 2005. 21 It is based on the quantitative measurement of agglutination caused by the reaction of albumin and polyclonal anti‐albumin antibodies.…”

Section: Introductionmentioning

confidence: 99%

Establishment of a reference interval for urinary albumin‐to‐creatinine ratio in dogs

Falus

Vizi

Szabó

et al. 2022

Veterinary Clinical Pathol

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Background Albuminuria is an important marker of renal damage and can precede proteinuria; thus, it can be a useful analyte in the early diagnosis of kidney diseases. Albuminuria has also been found in dogs with hypertension, inflammatory, infectious, and neoplastic diseases. Objectives The aim of this study was to establish a reference interval (RI) for albuminuria in dogs. Methods One hundred sixty‐four clinically healthy dogs were enrolled in the study. Urinary albumin was determined by the immunoturbidimetric method, and albumin excretion was expressed as the urinary albumin‐to‐creatinine (UAC) ratio. The RI for UAC was established. Results After exclusions, 124 dogs from 32 breeds remained. The median UAC of the study population was 3.0 mg/g (range: 0‐48). The RI was defined as 0‐19 mg/g (with a 90% CI for the upper limit of 13‐28 mg/g). No significant difference was found between male and female dogs or between different age and body weight groups. The results of Sighthounds (n = 30) and Beagle dogs (n = 23) did not differ from the other breeds. Conclusion The canine RI of UAC is similar but somewhat narrower than the human RI.

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Section: Introductionmentioning

confidence: 99%

Establishment of a reference interval for urinary albumin‐to‐creatinine ratio in dogs

Falus

Vizi

Szabó

et al. 2022

Veterinary Clinical Pathol

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“…Therefore, urinary microalbumin has been proposed as an early sign of kidney damage in people who have a risk of chronic kidney disease and cardiovascular disease in nondiabetic individuals. 6 There are many methods that have been proposed to determine the amount of albumin in urine such as enzyme-linked immunosorbent assay (ELISA), 7 fluorescence, 8,9 immunoturbidimetry (IT), 10,11 immunonephelometry (IN), 12 radioimmunoassay (RIA), 13 and chemiluminescence immunoassay (CLIA). 14,15 Although these methods are high sensitivity and specificity, they have some limitations such as health hazards, being time-consuming, labor-intensive protocols, and requiring experienced technicians.…”

Section: Introductionmentioning

confidence: 99%

A silicon nitride ion sensitive field effect transistor‐based immunosensor for determination of urinary albumin

Saengdee

Thanapitak

Ongwattanakul

et al. 2021

Electrochemical Science Adv

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The development of an immunosensor-based biosensor to detect the human serum albumin (HSA) was developed as a low-cost and label-free electrical detection through silicon nitride ion-sensitive field-effect transistor (Si 3 N 4 -ISFET).This sensor was functionalized using 3-Aminopropyltriethoxysilane (APTES). The silanized Si 3 N 4 -ISFET surface was covalently linked with antibody against HSA (Anti-HSA) via glutaraldehyde (GA) to function as an HSA immunosensor for detection of HSA. The interaction between HSA and Anti-HSA on this device is monitored by changing of the gate potential at a constant drain current of 50 μA. The relationship between HSA concentration and the gate potential change of HSA immunosensor is linear up to 500 μg/ml with a detection limit of 5 μg/ml. This HSA immunosensor offers a good precision in both intra-and inter-assay with a relative standard deviation of 6.61%, 7.48%, 10.69%, and 9.67%, 13.16%, 15.52% for 100, 200, and 400 μg/ml. The average percentage of recovery assay for the concentration of 32.1, 102.1, and 202.1 μg/ml is 96.55%. This sensor also provides its satisfactory selectivity when testing with Bence Jones protein solution. In addition, the results that was done by this HSA immunosensor is similar to the conventional method using immunoturbidimetric detection with a linear regression of 0.99; n = 40 with a paired sample t-test of <0.01. Thus, this HSA immunosensor could be applied to urinary microalbumin detection because of its simplicity and satisfactory correlation value.

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“…9 The standard analysis for urinary albuminuria in clinical laboratories uses two separate analytical methods, one to measure albumin and the other to measure creatinine. Albumin is usually quantified using immunochemical methods, such as immunoturbidimetry, 10 immunonephelometry, 11 enzyme-linked immunosorbent assays (ELISA), 12 or radial immunodiffusion. 13 On the other hand, the standard method for quantifying creatinine is based on the Jaffe's reaction, arguably the oldest clinical chemistry method in use, 14 in which creatinine reacts with picric acid in an alkaline solution to form an orange to red complex, which is then measured by spectrophotometry.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous quantitation of urinary albumin and creatinine for rapid clinical albuminuria diagnostics using high-throughput paper spray mass spectrometry

Pereira,

Robinson,

Gill

2024

Analyst

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Evaluation of five immunoturbidimetric assays for urinary albumin quantification and their impact on albuminuria categorization

Cited by 12 publications

References 13 publications

Establishment of a reference interval for urinary albumin‐to‐creatinine ratio in dogs

Establishment of a reference interval for urinary albumin‐to‐creatinine ratio in dogs

A silicon nitride ion sensitive field effect transistor‐based immunosensor for determination of urinary albumin

Simultaneous quantitation of urinary albumin and creatinine for rapid clinical albuminuria diagnostics using high-throughput paper spray mass spectrometry

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