Evaluation of fish sperm quality

Fauvel, Christian; Suquet, Marc; Cosson, Jacky

doi:10.1111/j.1439-0426.2010.01529.x

Cited by 154 publications

(127 citation statements)

References 53 publications

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“…We placed a sample of the ejaculate of one male in the center of a 280-μL water droplet harbored in a concave microscope slide. The droplet was framed with a concentrated glucose solution to provide a dilution gradient attracting sperm toward the edges of the droplet (27). Upon contact with water, sperm were activated and dispersed within the water droplet, and longer-lived sperm were expected to reach the outer edge of the water droplet more frequently than short-lived sperm.…”

Section: Significancementioning

confidence: 99%

Haploid selection within a single ejaculate increases offspring fitness

Alavioon

Hotzy

Nakhro

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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An inescapable consequence of sex in eukaryotes is the evolution of a biphasic life cycle with alternating diploid and haploid phases. The occurrence of selection during the haploid phase can have farreaching consequences for fundamental evolutionary processes including the rate of adaptation, the extent of inbreeding depression, and the load of deleterious mutations, as well as for applied research into fertilization technology. Although haploid selection is well established in plants, current dogma assumes that in animals, intact fertile sperm within a single ejaculate are equivalent at siring viable offspring. Using the zebrafish Danio rerio, we show that selection on phenotypic variation among intact fertile sperm within an ejaculate affects offspring fitness. Longer-lived sperm sired embryos with increased survival and a reduced number of apoptotic cells, and adult male offspring exhibited higher fitness. The effect on embryo viability was carried over into the second generation without further selection and was equally strong in both sexes. Sperm pools selected by motile phenotypes differed genetically at numerous sites throughout the genome. Our findings clearly link within-ejaculate variation in sperm phenotype to offspring fitness and sperm genotype in a vertebrate and have major implications for adaptive evolution.

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Section: Significancementioning

confidence: 99%

Haploid selection within a single ejaculate increases offspring fitness

Alavioon

Hotzy

Nakhro

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

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“…Therefore, many researchers have still relied on more subjective methods of motility assessment (Rurangwa et al, 2004;Fauvel et al, 2010;Kalbassi et al, 2013). The subjective methods of motility analysis are practical and simple; however, their accuracy depends on the experience of the evaluator and is more prone to human error .…”

Section: Introductionmentioning

confidence: 99%

Contribution of the CASA system, based on open source software, to the assessment of sperm characteristics: informetric and usage-based approaches

et al. 2017

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-By using informetric tools, we evaluated the contribution and impact of the open source computer-assisted sperm analysis (CASA) software on sperm studies, especially pertaining to fish. The article was cited 125 times between 2007 and 2014 in four publication types (articles, doctoral dissertations, master's theses, and books). Among articles, the number of citations increased at rate of three citations per year and the calculated h-index was 17. Of the studies that cited Wilson-Leedy and Ingermann (2007), fish were the main group of organisms studied (61 articles); of these, 34 articles used the open source CASA, covering 23 marine or freshwater species. Studies conducted in 12 countries were published in 21 journals. Four ways of knowledge sharing were identified: by direct partnership with the system developers; by knowledge multipliers, who worked directly with the system developers; by groups that adopted the system by their own initiative; and through expansion of cooperation networks with autodidacts. In regard to specific analytical procedures, motility, curvilinear velocity, average path velocity, and straight-line velocity were the main CASA parameters used to describe sperm movements. As expected, the usage of this tool has been increasing over the years, being a reliable and versatile alternative to more costly similar platforms. However, the potential of the CASA system has not been completely explored and it is our assessment that some results produced by CASA are poorly understood or even misunderstood.Key Words: computational analysis, fish, motility, scientometry, spermatozoa Revista Brasileira de Zootecnia

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“…From a biological standpoint, sperm quality could be defined as the ability ofthe spermatozoa to reach and fertilize the oocyte (Fauvel et al, 2010). In this respect, any quantifiable parameter directly correlated with the fertilization capacity could potentially be used as a sperm quality indicator.…”

Section: Sperm Quality In Fishmentioning

confidence: 99%

“…At this stage, the sperm cells are quiescent but already prepared for accomplishing their fertilizing task (Fauvel et al, 2010). However, sperm activation will be an essential requirement in the pursuit of female gametes, and spermatozoa exploit their swimming ability in order to encounter the oocyte as fast as possible (Cosson et al, 2008a).…”

Section: Factors Determining Sperm Activationmentioning

confidence: 99%

“…Some of these parameters can be scored relatively easily and are commonly used because no expensive equipment is needed and they are quick and easy to assess. On the other hand, other biomarkers need sophisticated laboratory analyses or expensive equipment, thus at themoment the application of these is not directly available to fish farms.On the other hand, despite it having been reported that it is the set of sperm biomarkers as a whole that contribute to determining sperm quality and that each one, by itself, is not a single predictive parameter, sperm motility is currently considered the most useful tool to assess sperm quality Fauvel et al, 2010; Boryspholets et al, 2013). However, the most used technique to assess sperm motion has been subjective evaluation, and some problems have emerged from this method.…”

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confidence: 99%

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Sperm physiology and quality in two marine teleosts: Anguilla anguilla & Takifugu niphobles

Albiach¹

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ACKNOWLEDGEMENTSMuchos años han pasado desde que en aquel verano de 2007, cuando aún era un biólogo casi recién salido del horno, empecé mi carrera investigadora gracias a una beca en el grupo de investigación en el que hoy, unos años más viejuno, presento mi pequeña aportación a la ciencia. Durante todo este periodo he pasado buenos y malos momentos, mejores y peores rachas, pero sobretodo, he vivido una experiencia difícil de olvidar, o más bien inolvidable, en la que he podido madurar como persona y como investigador gracias a todas las personas que me han acompañado durante estos maravillosos años. Son muchos los agradecimientos que tengo que dar y poca la memoria, así que pido perdón por si alguien cae en el olvido, aunque no hay nada que no se pueda solucionar con una cerveza fresquita! En primer lugar, quería dar gracias a mi familia, que aunque nunca se lo diga y muchísimas veces parezca un "desapegao"… les quiero un montón. Ya hacen 33 primaveras que "el pare" y "la máaaama" (si, con acento) me trajeron al mundo, porque ya sabemos que lo que va primero es el oocito y el espermatozoide (Chapter 5… jjjjj), así que mil gracias por el cariño y el apoyo incondicional que me habéis dado durante todos estos años. En el mismo pack entra mi querida hermana "mongola" (uooooooooo… esto queda publicado en libro teta!!!), que también ha estado ahí y siempre me ha echado un cable cuando ha podido, aunque sea para decir… "tete no llegues tarde que mañana tienes laboratorio!!!" (si el microscopio o el citómetro hablaran...).A Paty (mi monito), por tu amor, comprensión y apoyo infinito durante todos estos años. Aún recuerdo el primer día que te conocí andando por el departamento y tu sonrisa se quedó grabada en mi memoria (que por cierto te invité a comer y me dijiste que… noooooooo!! toma xafun!!). Supongo que nos empezamos a querer en Monfragüe viendo y descubriendo pajarines mientras oíamos como cantaba el búhito, así que mil gracias por estar conmigo en los buenos y malos momentos, por ser un apoyo incondicional para mí, por quererme, y por todos estos años de sonrisas que hemos pasado juntos… que han sido muchos y muy bonitos.A mis amigos de toda la vida!!! Of course!!! que han estado ahí y estarán durante muchos años. Realmente es un grupo heterogéneo, que va desde los gorilillas de mi equipo de balonmano hasta algunos con los que empecé a gatear en preescolar. Que bien me lo he pasado en los torneos balonmaneros de San Esteve, donde dimos nuestros primeros pasos internacionales en materia de balonmano y en materia de bebercios!!! Mención especial merece mi amigo Caniggi, que a pesar de su estresante vida (tiene una pizzería, un huerto, un trabajo, un perro… y encima novia!!!) aun saca ratillos para jugar a pádel (hombre él jugar jugar… no juega… jjjjj) y recordad viejos tiempos de la infancia cuando aún éramos unos críos.Mucha gente es con la que he compartido laboratorio y a la que quería dar las gracias de manera especial. Realmente no estaría bien hablar de compañeros de laboratorio, porque ahora muchos de ell...

show abstract

Evaluation of fish sperm quality

Cited by 154 publications

References 53 publications

Haploid selection within a single ejaculate increases offspring fitness

Haploid selection within a single ejaculate increases offspring fitness

Contribution of the CASA system, based on open source software, to the assessment of sperm characteristics: informetric and usage-based approaches

Sperm physiology and quality in two marine teleosts: Anguilla anguilla & Takifugu niphobles

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