Evaluation of ferric oxide and ferric citrate for their effects on fermentation, production of sulfide and methane, and abundance of select microbial populations using in vitro rumen cultures

Wu, Hao; Meng, Qingxiang; Yu, Zhongtang

doi:10.1016/j.biortech.2016.03.126

Cited by 9 publications

(22 citation statements)

References 34 publications

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“…; Patra and Yu ; Wu et al . ), the concentration of total VFA was not altered by any of the treatments. Thus, the combination of saponin and nitrate can decrease CH 4 emission without adversely affecting feed digestion or fermentation.…”

Section: Discussionmentioning

confidence: 75%

“…Ferric citrate was used because it was found to be more effective than ochre in decreasing CH 4 and sulphide production (Wu et al . ). Ferric citrate (C 6 H 5 FeO 7 ) and Quillaja saponin (from the bark of Quillaja saponaria Molina plants) were purchased from Sigma‐Aldrich (St. Louis, MO).…”

Section: Methodsmentioning

confidence: 97%

“…The concentrations were chosen based on previous research (Patra and Yu ; Wu et al . ). Two healthy lactating Jersey cows, fitted with permanent rumen fistula, were used as donors of rumen fluid.…”

Section: Methodsmentioning

confidence: 97%

“…Fresh rumen fluid was collected to prepare the composite inoculum essentially as described previously (Wu et al . ). Briefly, rumen content was collected from two cows and squeezed through four layers of cheesecloth.…”

Section: Methodsmentioning

confidence: 97%

“…All the qPCR assays were performed in three replicates essentially as described previously (Wu et al . ).…”

Section: Methodsmentioning

confidence: 97%

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Ferric citrate, nitrate, saponin and their combinations affect in vitro ruminal fermentation, production of sulphide and methane and abundance of select microbial populations

Meng

Zhou

et al. 2019

Journal of Applied Microbiology

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Aims This study investigated the effects of ferric citrate, nitrate and saponin, both individually and in combination, on sulphidogenesis, methanogenesis, rumen fermentation and abundances of select microbial populations using in vitro rumen cultures. Methods and Results Ferric citrate (50 mg l−1), Quillaja saponin (0·6 g l−1) and sodium nitrate (5 mmol l−1) were used in in vitro ruminal fermentation. Ferric citrate alone, its combination with saponin and/or nitrate lowered the aqueous sulphide concentration and total sulphide production. Methane production was suppressed by nitrate alone (by up to 32·92%), its combination with saponin (25·04%) and with both saponins with nitrate (25·92%). None of the treatments adversely affected feed digestion or rumen fermentation. The population of sulphate‐reducing bacteria was increased by nitrate and saponin individually, while that of total Archaea was decreased by nitrate alone and the combination of the three inhibitors. Conclusions Nitrate and its combination with saponin or both ferric citrate and saponin substantially decreased methane production. Most importantly, the decreased methane production was not at the expense of feed digestion or fermentation. Sulphidogenesis from the sulphate present in the high‐sulphur diets can be suppressed competitively by ferric citrate, although it was elevated by saponin and nitrate. Significance and Impact of the Study The results of this study demonstrated that combinations of certain methane inhibitors, which have different mechanisms of antimethanogenic actions or inhibit different guilds of microbes involved in methane production and sulphate reduction, can be more effective and practical than individual inhibitors, not only in mitigating enteric methane emission but also in lowering the risk of sulphur‐associated polioencephalomalacia in feedlot cattle fed high sulphur diets.

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Section: Discussionmentioning

confidence: 75%

Section: Methodsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 97%

“…All the qPCR assays were performed in three replicates essentially as described previously (Wu et al . ).…”

Section: Methodsmentioning

confidence: 97%

See 3 more Smart Citations

Ferric citrate, nitrate, saponin and their combinations affect in vitro ruminal fermentation, production of sulphide and methane and abundance of select microbial populations

Meng

Zhou

et al. 2019

Journal of Applied Microbiology

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Effect of Irpex lacteus, Pleurotus ostreatus and Pleurotus cystidiosus pretreatment of corn stover on its improvement of the in vitro rumen fermentation

et al. 2018

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Potential of guava leaves for mitigating methane emissions and modulating ruminal fermentation characteristics and nutrient degradability

Al‐Sagheer

Elwakeel

Ahmed

et al. 2018

Environ Sci Pollut Res

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Guava leaves (Psidium guajava, GL), a high-phenolic- and flavonoid-containing plant resource capable of substituting the high-quality forage, may help in mitigating ruminal methane (CH) emission without adverse impact on nutrient degradability if supplemented at an appropriate level. In order to test this hypothesis, rumen fermentation, CH production, and nutrient degradability of GL either solely or as a substitute of berseem hay (Trifolium alexandrinum, BH) were evaluated in a diet containing 50:50 concentrate to roughage. Five different levels of GL (0, 12.5, 25, 37.5, and 50%) were tested in vitro after 24 h incubation using a semi-automated gas production (GP) system. The current findings indicated that merely the presence of GL resulted in significantly lower values for cumulative GP (P < 0.001), CH emission (P < 0.05), truly degraded dry matter (TDDM; P < 0.001), truly degraded organic matter (TDOM; P < 0.001), and ammonia nitrogen (NH-N) concentration (P < 0.001); however, pH (P < 0.001) and partitioning factor (P < 0.001) were higher. The total and individual volatile fatty acid (VFA) concentrations were drastically declined with GL as compared to BH (P < 0.05). A negative linear correlation was recorded between the levels of GL and GP including CH production (P < 0.05). The addition of GL up to 25% did not pose any negative effect on both TDDM and TDOM values along with NH-N concentration. In addition, the inclusion of GL up to 25% did not affect the total or individual VFA concentration. Conclusively, in a medium concentrate diet, use of 25% GL and 25% BH in animal diet could be a promising alternative for mitigating the CH production without any deleterious effect on nutrient degradability.

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Evaluation of ferric oxide and ferric citrate for their effects on fermentation, production of sulfide and methane, and abundance of select microbial populations using in vitro rumen cultures

Cited by 9 publications

References 34 publications

Ferric citrate, nitrate, saponin and their combinations affect in vitro ruminal fermentation, production of sulphide and methane and abundance of select microbial populations

Ferric citrate, nitrate, saponin and their combinations affect in vitro ruminal fermentation, production of sulphide and methane and abundance of select microbial populations

Effect of Irpex lacteus, Pleurotus ostreatus and Pleurotus cystidiosus pretreatment of corn stover on its improvement of the in vitro rumen fermentation

Potential of guava leaves for mitigating methane emissions and modulating ruminal fermentation characteristics and nutrient degradability

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