Evaluation of Fecal Coliforms in Effluents of a Swine Exploitation Subject to a Tubular Biodigester Treatment With Stacionary Freight System

Abstract

ABSTRACT. This research was conducted in order to evaluate the effect of plastic biodigester semicontinuous flow of bacteriological parameters (coliforms) in the treatment of effluents in small-scale pig farm. Three points within the system were used for sampling: drinking water pigs, pig slurry (water entering the digester), Bio (water that emerges from the digester), every 21 days over a period of 70 days, from July 2015. the samples were subjected to analysis to determine total and fecal coliforms by the method of Most Probable Number (MPN). The study revealed that drinking water samples from animals tested negative microbiological results, while the purines and the biol positive microbiological results were obtained. It was found the presence of coliform species: Proteus spp, Escherichia coli, Klebsiella, Citrobacter freundii. However, samples obtained before entering the digester (B) with then taken anaerobic process (C), maintained the maximum bacterial load, with no statistically significant differences (p> 0.05). RESUMEN.El presente trabajo de investigación fue realizado con el objetivo de evaluar el efecto del biodigestor tubular plástico de flujo semi-continuo sobre los parámetros bacteriológicos (contenido de coliformes fecales) en el tratamiento de efluentes en explotación porcina a pequeña escala. Tres puntos dentro del sistema fueron utilizados para la toma de muestras: agua de bebida de los cerdos, purines de cerdo (agua que ingresa al biodigestor), biol (agua que emerge del biodigestor), cada 21 días en un periodo de 70 días, a partir del mes de julio de 2015. Las muestras fueron sometidas a análisis para determinar los coliformes totales y fecales por el método del Número Más Probable (NMP). El estudio reveló que las muestras de agua del bebedero de los animales arrojaron resultados microbiológicos negativos, en tanto que de los purines y el biól obtuvieron resultados positivos. Se pudo constatar la presencia de coliformes de las especies: Proteus spp, Escherichia coli, Klebsiella, Citrobacter freundii. Sin embargo, las muestras obtenidas antes de ingresar al biodigestor (B) con las tomadas luego del proceso anaeróbico (C), mantuvieron la máxima carga bacteriana, no encontrándose diferencias estadísticamente significativas (p>0,05).

Genetic Characterization of Anaerobic Micro-Organisms Applied to Wastewater Treatment: An Alternative in Areas of Height

“…Anaerobic digestion is reduced to four phases: hydrolysis, acidogenesis, acetogenesis and methanogenesis; within the methanogenic bacteria, four genera stand out: Methanococcus, Methanebacterium, Methanosacosin, and Methanospirillum which transform the organic material present in wastewater by catabolizing acetate and monocarbon compounds to produce methane gas and generate biogas [5], [6]. The specific methanogenic activity (SMA) is carried out to evaluate the microbial activity of converting the organic substrate into methane [7]; this activity establishes the maximum capacity of chemical oxygen demand (COD) removal that allows to establish the minimum and the maximum organic concentration load to be applied in a reactor to ensure the reduction of organic matter into biogas [8]; the determination of biochemical oxygen demand (BOD), COD and suspended solids, both sedimentable and non-sedimentable (SS), are sufficient to assess the organic material removal.…”