Evaluation of fatty acid profile and biodiesel properties of microalga Scenedesmus abundans under the influence of phosphorus, pH and light intensities

Mandotra, S. K.; Kumar, Pankaj; Suseela, M. R.; Nayaka, Sanjeeva; Ramteke, Pramod W.

doi:10.1016/j.biortech.2015.11.042

Cited by 132 publications

(45 citation statements)

References 36 publications

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“…It has been documented that high lipid content is usually accompanied by lower growth rates under different stresses which often lead to decreased biomass and hence overall lipid productivity (Dhup and Dhawan 2014; Mandotra et al 2016). However as lipid biosynthesis in C. cohnii does not follow this usual pattern, and continue to accumulate regardless of LN or HN.…”

Section: Discussionmentioning

confidence: 99%

Growth kinetics, fatty acid composition and metabolic activity changes of Crypthecodinium cohnii under different nitrogen source and concentration

et al. 2017

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The effect of varying concentrations of the nitrogen source on the growth kinetics, lipid accumulation, lipid and DHA productivity, and fatty acid composition of C. cohnii was elucidated. Growth of C. cohnii was in three distinct growth stages: cell growth, lipid accumulation and a final lipid turnover stage. Most of lipids were accumulated in lipid accumulation stage (48–120 h) though, slow growth rate was observed during this stage. NaNO3 supported significantly higher lipid content (26.9% of DCW), DHA content (0.99 g/L) and DHA yield (44.2 mg/g glucose) which were 2.5 to 3.3-folds higher than other N-sources. The maximum level of C16–C18 content (% TFA) was calculated as 43, 54 and 43% in lipid accumulation stage under low nitrogen (LN, 0.2 g/L), medium nitrogen (MN, 0.8 g/L) and high nitrogen (HN, 1.6 g/L) treatments, respectively. Cultures with LN, by down-regulating cell metabolism, trigger onset of lipogenic enzymes. Conversely, NAD+/NADP+-dependent isocitrate dehydrogenase (NAD+/NADP+-ICDH) were less active in LN than HN treatments which resulted in retardation of Kreb’s Cycle and thereby divert citrate into cytoplasm as substrate for ATP-citrate lyase (ACL). Thereby, ACL and fatty acid synthase (FAS) were most active in lipid accumulation stage at LN treatments. Glucose-6-phosphate dehydrogenase (G6PDH) was more active than malic enzyme (ME) in lipid accumulation stage and showed higher activities in NaNO3 than other N-sources. This represents that G6PDH contributes more NADPH than ME in C. cohnii. However, G6PDH and ME together seems to play a dual role in offering NADPH for lipid biosynthesis. This concept of ME together with G6PD in offering NADPH for lipogenesis might be novel in this alga and needed to be explored.

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Section: Discussionmentioning

confidence: 99%

Growth kinetics, fatty acid composition and metabolic activity changes of Crypthecodinium cohnii under different nitrogen source and concentration

et al. 2017

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“…Thus, adequate light intensity favors the overproduction of microalgal lipids. For example, high light conditions trigger the formation of neutral lipids in the microalgae Scenedesmus abundans and Nannochloropsis sp., as well as Botryococcus braunii and a further Botryococcus species (Kojima and Zhang, 1999; Pal et al, 2011; Yeesang and Cheirsilp, 2011; Mandotra et al, 2016). …”

Section: Lipid Accumulation In Oleaginous Microorganisms Under Differmentioning

confidence: 99%

Reactive Oxygen Species-Mediated Cellular Stress Response and Lipid Accumulation in Oleaginous Microorganisms: The State of the Art and Future Perspectives

et al. 2017

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Microbial oils, which are mainly extracted from yeasts, molds, and algae, have been of considerable interest as food additives and biofuel resources due to their high lipid content. While these oleaginous microorganisms generally produce only small amounts of lipids under optimal growth conditions, their lipid accumulation machinery can be induced by environmental stresses, such as nutrient limitation and an inhospitable physical environmental. As common second messengers of many stress factors, reactive oxygen species (ROS) may act as a regulator of cellular responses to extracellular environmental signaling. Furthermore, increasing evidence indicates that ROS may act as a mediator of lipid accumulation, which is associated with dramatic changes in the transcriptome, proteome, and metabolome. However, the specific mechanisms of ROS involvement in the crosstalk between extracellular stress signaling and intracellular lipid synthesis require further investigation. Here, we summarize current knowledge on stress-induced lipid biosynthesis and the putative role of ROS in the control of lipid accumulation in oleaginous microorganisms. Understanding such links may provide guidance for the development of stress-based strategies to enhance microbial lipid production.

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“…Chlorophyll content is a specific parameter related to the photosynthesis process, which is involved in the PSl and PSll complexes that utilize photon energy to transfer electrons toward the electron chain to produce a transmembrane gradient of the redox electrical potential and an H + concentration that catalyzes ATP synthesis . At a pH value of 7.8; an increase in cell growth of C. reinhardtii was observed with a significant accumulation of chlorophyll (1.75‐fold) when compared with pH 7, while at pH 8.5 a decrease in both parameters was observed.…”

Section: Discussionmentioning

confidence: 99%

pH effects on the lipid and fatty acids accumulation in Chlamydomonas reinhardtii

Ochoa-Alfaro

Gaytán-Luna

González‐Ortega

et al. 2019

Biotechnology Progress

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pH variations influence the delivery of essential nutrients and CO2 solubility, which impact algae metabolism. In this study the microalgal growth and chlorophyll, lipid, and fatty acids content; along with the expression of some genes implicated in the biosynthesis of lipids were examined in Chlamydomonas reinhardtii subjected to pH values of 7.0, 7.8, and 8.5. At pH 7.8 an increase in cell growth was observed with a significant accumulation of chlorophyll (1.75‐fold) when compared with growth at pH 7, while at pH 8.5 a sharp decrease in both parameters was observed when compared with the other pH values tested. Lipid content increased 3.0 (14.81% of dry cell weight, dcw) and 2.3 times (11.43% dcw) at pH 7.8 and 8.5, respectively, when compared with the experiment at pH 7 (4.97% dcw). The compositions of major fatty acids in the strains growing at pH 7.0, 7.8, or 8.5 were 25.7, 28.0, and 32.1% for palmitic acid; 17.3, 14.7, and 25.7% for oleic acid; and 9.8, 12.1, and 4.6% for linoleic acid; respectively. qRT‐PCR analysis showed that the transcripts of ß‐carboxyltransferase, Acyl carrier protein 1, acyl‐ACP thiolase 1, acyl‐sn‐glycerol‐3‐phosphate acyltransferase, and diacylglycerol acyl transferase isoform 3 were significantly induced at pH 7.8 when compared with the other two pH conditions. These results indicate that the induction of genes implicated in the early and final steps of lipid biosynthesis contributes to their accumulation in the stationary phase. Our research suggests that a pH of 7.8 might be ideal to maximize growth and lipid accumulation.

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Evaluation of fatty acid profile and biodiesel properties of microalga Scenedesmus abundans under the influence of phosphorus, pH and light intensities

Cited by 132 publications

References 36 publications

Growth kinetics, fatty acid composition and metabolic activity changes of Crypthecodinium cohnii under different nitrogen source and concentration

Growth kinetics, fatty acid composition and metabolic activity changes of Crypthecodinium cohnii under different nitrogen source and concentration

Reactive Oxygen Species-Mediated Cellular Stress Response and Lipid Accumulation in Oleaginous Microorganisms: The State of the Art and Future Perspectives

pH effects on the lipid and fatty acids accumulation in Chlamydomonas reinhardtii

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