Evaluation of Fasting and Postprandial Total Serum Bile Acid Concentration in Dogs with Hepatobiliary Disorders

Jensen, A. L.

doi:10.1111/j.1439-0442.1991.tb01009.x

Cited by 15 publications

(12 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The sensitivity of pre-and postprandial SBA to detect hepatic dysfunction is discussed controversially; some authors have shown little difference between both parameters for most disorders, 22 whereas others have demonstrated PP SBA to be superior. 2,23,24 A recent study found baseline SBA concentrations to have a sensitivity of 92.2% for the detection of a congenital PSS and 81% for acquired shunting. 9 In our study, none of the dogs with tracheal collapse had increased baseline SBA and 2 of 11 dogs with a PSS were missed with only a fasting SBA measurement.…”

Section: Discussionmentioning

confidence: 99%

Comparison of Postprandial and Ceruletide Serum Bile Acid Stimulation in Dogs

Bridger

Glanemann

Neiger

2008

Veterinary Internal Medicne

View full text Add to dashboard Cite

Background: Postprandial (PP) serum bile acid (SBA) stimulation is an important test for detecting hepatic dysfunction in dogs. However, this test is influenced by numerous variables, and a standardized approach using an injectable cholecystokinin analog (ceruletide) may be advantageous.Hypothesis: Ceruletide SBA stimulation test is more sensitive than PP SBA stimulation in dogs. Animals: Animals with portosystemic shunt (PSS) (n 5 11) and dogs with upper respiratory disease (URD) (n 5 9) were investigated. Healthy dogs (n 5 13) and dogs with other diseases (n 5 17) served as controls.Methods: All dogs underwent SBA stimulation with food and ceruletide. Stimulation blood samples were drawn at 60/120 minutes and 20/30/40 minutes, respectively. Results were compared statistically, and the sensitivity and specificity were determined with receiver-operating characteristic curves.Results: Stimulated SBA were significantly higher in both study groups than in controls. For dogs with PSS, the sensitivity and specificity (435 mmol/L) were 100% postprandially (120 minutes) and 91 and 100%, respectively, postceruletide (30 minutes). The difference between these values was not statistically significant. For dogs with URD, the sensitivity and specificity (422 mmol/L) were 44 and 88% postprandially (120 minutes) and 100 and 88% postceruletide (30 minutes).Conclusions and Clinical Importance: Ceruletide SBA stimulation circumvents exogenous and endogenous influences associated with PP SBA stimulation. The results indicate that ceruletide SBA stimulation performs as well as PP SBA stimulation in dogs with PSS and is more sensitive for the detection of hepatic dysfunction in dogs with URD.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison of Postprandial and Ceruletide Serum Bile Acid Stimulation in Dogs

Bridger

Glanemann

Neiger

2008

Veterinary Internal Medicne

View full text Add to dashboard Cite

show abstract

“…In the enterohepatic circulation,>95% of the bile acids are removed from portal blood by hepatocytes and recycled back into the biliary system 1 . An increase in SBA concentration can be expected in cases of: (1) portovascular anomalies such as PSS, (2) parenchymal hepatic disease (PHD), and (3) cholestasis, in which there is leakage of bile acids from hepatocytes into the systemic blood circulation 10 …”

Section: Introductionmentioning

confidence: 99%

Sensitivity and specificity of fasting ammonia and serum bile acids in the diagnosis of portosystemic shunts in dogs and cats

Ruland

Fischer

Hartmann

2010

Veterinary Clinical Pathol

View full text Add to dashboard Cite

show abstract

“…This resulted in a lack of normal distribution to our population. The effects of serum hemolysis and lipemia, which have been previously shown to falsely decrease and increase bile acid concentrations, respectively, as measured by enzymatic assay (Jensen, 1991; Solter, Hoffmann & Hoffman, 2000), were also not directly assessed in this study.…”

Section: Discussionmentioning

confidence: 94%

“…To minimize variables, serum samples were frozen and stored after collection so that all testing could be performed on the same day, immediately after thawing. Bile acids can be stored frozen at −20 °C for at least 3 months with little variability (Jensen, 1991; Olsson, 1988). …”

Section: Discussionmentioning

confidence: 99%

“…Within the liver, bile acids are removed from portal blood by hepatocytes and recycled back into the biliary system (termed enterohepatic circulation). An increase in SBA can therefore be expected with hepatic, biliary, or portal disorders that limit hepatic portal blood flow or hepatocellular uptake (Jensen, 1991). In dogs, pre- and postprandial SBA have repeatedly been found to be reliable markers for the diagnosis of hepatic and biliary dysfunction and disorders of the portal vasculature, such as portosystemic shunts (PSS) and congenital portal vein hypoplasia with secondary microvascular dysplasia (PVH-MVD).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of a semiquantitative SNAP test for measurement of bile acids in dogs

Seibert

Tobias

Reed

et al. 2014

PeerJ

View full text Add to dashboard Cite

Background. Serum bile acids (SBA) are used as a routine screening tool of liver function in dogs. Serum samples are usually shipped to a referral laboratory for quantitative analysis with an enzymatic chemistry analyzer. The canine SNAP Bile Acids Test (SNAP-BAT) provides an immediate, semi-quantitative measurement of bile acid concentrations in-house. With the SNAP-BAT, bile acids concentrations of 5–30 µmol/L are quantified, and results outside of that range are classified as <5 or >30 µmol/L. Agreement of the SNAP-BAT with the enzymatic method has not been extensively investigated.Objectives. The purposes of this prospective clinical study were to assess the precision of the SNAP-BAT and determine agreement of SNAP-BAT with results from an in-house chemistry analyzer.Methods. After verifying intra-assay precision of the SNAP-BAT, a prospective analysis was performed using blood samples collected from 56 dogs suspected to have liver disease. Each sample was analyzed with an enzymatic, in-house chemistry analyzer and the SNAP-BAT. Agreement between the two methods was statistically assessed using the κ index of agreement.Results. Intra-assay variability was minimal. The κ index for agreement between the SNAP-BAT and routine chemistry analyzer was between 0.752 and 0.819, indicating substantial to near perfect agreement.Conclusions. The SNAP-BAT is a highly accurate, semi-quantitative test that yields immediate results, and has very little intra-assay variability, particularly for results >30 µmol/L.

show abstract

Evaluation of Fasting and Postprandial Total Serum Bile Acid Concentration in Dogs with Hepatobiliary Disorders

Cited by 15 publications

References 11 publications

Comparison of Postprandial and Ceruletide Serum Bile Acid Stimulation in Dogs

Comparison of Postprandial and Ceruletide Serum Bile Acid Stimulation in Dogs

Sensitivity and specificity of fasting ammonia and serum bile acids in the diagnosis of portosystemic shunts in dogs and cats

Evaluation of a semiquantitative SNAP test for measurement of bile acids in dogs

Contact Info

Product

Resources

About