Evaluation of faecal flotation methods followed by species-specific PCR for detection of Echinococcus multilocularis in the definitive hosts

Széll, Z.; Sréter-Lancz, Zsuzsanna; Sréter, T.

doi:10.2478/s11686-014-0248-9

Cited by 17 publications

(8 citation statements)

References 32 publications

(33 reference statements)

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“…Consequently, subclinical trematode and lung nematode infections may not be diagnosed. Therefore, animals involved in future reintroductions should be carefully investigated for parasites using a combination of faecal concentrating techniques (sedimentation, lotating and Baermann) and even molecular or serological diagnosis tests, [67][68][69][70] according to standard procedures of good veterinary practice. 71 The results of these survey suggest that it may be diicult to control parasites (mainly the ones that shed eggs in animal faeces) for scimitar-horned oryx, Cuvier's gazelle and Mohor gazelle in zoological institutions, despite the preventive measures implemented and the administration of treatments when parasites are detected.…”

Section: Discussionmentioning

confidence: 99%

Survey of husbandry practices for bovidae in zoos: the importance of parasite management for reintroduction programmes

et al. 2019

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Animals from zoological institutions may be used for reintroductions. These individuals are considered healthy, but they are not necessarily free of parasites, despite the minimum husbandry standards required of zoological institutions as described in the European Association of Zoos and Aquaria guidelines. In this sense, parasitism has been identified as the cause of failure, or has added difficulties, in some reintroduction programmes. Here the authors attempt to summarise the risk of parasitism to animals originating from zoological institutions by analysing a questionnaire about parasite prevalence, sampling methods, treatment and control in three ungulates in European zoos. Completed questionnaires were received from 38 institutions (58.5 per cent response rate). Most of the responding institutions (97 per cent) detected the eggs of endoparasites in faeces, but only one reported ectoparasites. Most institutions followed a similar preventive schedule, with ivermectin as the preferred prophylactic treatment for parasites, commonly administered in food every six months. The frequent use of concentrating flotation techniques as the sole method to evaluate the presence of parasite eggs in faecal samples is not recommended because it fails to detect trematode and lung nematode infections, so it would be better to use flotation techniques together with sedimentation procedures or serological and molecular tests. The results suggest that parasite control in zoological institutions can be complicated, indicating the need to implement a specific management schedule for institutions involved in reintroduction projects.

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Section: Discussionmentioning

confidence: 99%

Survey of husbandry practices for bovidae in zoos: the importance of parasite management for reintroduction programmes

et al. 2019

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“…This is particularly important in the case of our non-detection of Echinococcus granulosis , an extremely pathogenic zoonotic parasite from dogs. The eggs of this species have a specific gravity too high (S.G. = 1.3−1.4) to easily float in the solutions used, and the sensitivity of the methods employed here range between only 3% and 33% for detection of this parasite [ 32 ]. Therefore, the results of this study should under no circumstances be interpreted as suggesting that there is no risk for hydatid disease from domestic dogs in Central Queensland.…”

Section: Discussionmentioning

confidence: 99%

A Survey of Intestinal Parasites of Domestic Dogs in Central Queensland

Gillespie

Bradbury

2017

TropicalMed

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Australia has a very high rate of dog ownership, which in some circumstances may lead to exposure to zoonotic parasitic diseases from those companion animals. Domestic dog faecal samples (n = 300) were collected from public spaces and private property in the greater Rockhampton (Central Queensland) region and tested for intestinal helminths and protozoa by direct microscopy, two flotation methods and a modified acid-fast stain for cryptosporidia. Intestinal parasites detected included hookworms (25%), Cystoisospora ohioensis complex (9%), Blastocystis hominis (3%), Giardia duodenalis (3%), Spirometra erinacei (1%) and Toxocara canis (1%), Sarcocystis spp. (2%), Cryptosporidium spp. (2%) and Cystoisospora canis (1%). One infection each with Trichuris vulpis, Dipylidium caninum and a protozoa belonging to the Entamoeba histolytica complex were identified. Sheather’s sucrose centrifugal flotation was more sensitive than saturated salt passive flotation, but no single test detected all cases of parasitic infection identified. The test methodologies employed are poor at recovering larva of Strongyloides stercoralis, Aleurostrongylus abstrussis and eggs of cestodes such as Echinococcus granulosis, so the potential presence of these parasites in Central Queensland domestic dogs cannot be excluded by this survey alone.

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“…Eggs from all the Toxocara positive samples detected by flotation/sedimentation were examined by one step PCR to determine the T. canis . To concentrate eggs, faecal samples were prepared with improved flotation method (Szell et al, 2014). When Toxocara -type eggs were detected microscopically, 1 ml of the upper part of the flotation from the centrifuge tube was transferred to the 15 ml falcon tube.…”

Section: Methodsmentioning

confidence: 99%

Detection and identification of Toxocara canis in infected dogs using PCR

Öge

Özbakış-Beceriklisoy

2019

Helminthologia

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SummaryFaecal samples were collected from 224 dogs (47 villages) in Ankara. Toxocara spp. eggs were diagnosed in faeces using centrifugal flotation and sedimentation methods. A total of 21 dogs (9.38 %) were positive for Toxocara spp. eggs. In this study, we used the PCR technique that, in combination with DNA sequencing, allows the detection and identification of T.canis eggs in faeces of infected dogs. For this purpose, the ATPase subunit-6 gene (mtDNA) was selected as a target for the amplification T. canis. The primers were used to amplify 217 bp region. Amongst 21 coproscopically detected Toxocara isolates from dogs, 5 (23.8 %) samples were PCR-positive for T. canis, and the remaining 16 samples were PCR-negative. Results indicate that PCR can detect Toxocara canis DNA in faeces of infected dogs, but efficacy was low when compare to sedimentation/flotation. PCR is additional test for diagnosing of this infection. But, the difficulties of identification based on PCR in faecal examinations need to be investigated further.

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Evaluation of faecal flotation methods followed by species-specific PCR for detection of Echinococcus multilocularis in the definitive hosts

Cited by 17 publications

References 32 publications

Survey of husbandry practices for bovidae in zoos: the importance of parasite management for reintroduction programmes

Survey of husbandry practices for bovidae in zoos: the importance of parasite management for reintroduction programmes

A Survey of Intestinal Parasites of Domestic Dogs in Central Queensland

Detection and identification of Toxocara canis in infected dogs using PCR

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