Evaluation of F1 and BC1 hybrids of <i>Lilium lancifolium</i> × Asiatic hybrid ‘Chianti’ by morphological analysis and fluorescence <i>in situ</i> hybridization

Ramzan, Fahad; Kim, Hyoung Tae; Shim, Kyung-ku; Younis, Adnan; Choi, Yu Hyeon; Hwang, Yoon‐Jung; Lim, Ki-Byung

doi:10.1080/14620316.2018.1432296

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…In lily, modern hybrid breeding exploit hybrid vigor of inter-sectional hybridization of Longiflorum, Oriental and Asiatic hybrids 47 . Breeders selects those hybrids for plant height; number of leaves, flowers and bulbils; leaf area; flower size; days to flowering and petal area which indicated that those traits were often found heterotic [48][49][50] .…”

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Transcriptome Analysis by RNA–Seq Reveals Genes Related to Plant Height in Two Sets of Parent-hybrid Combinations in Easter lily (Lilium longiflorum)

Howlader

Robin

Natarajan

et al. 2020

Sci Rep

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In this study, two different hybrids of Easter lily (Lilium longiflorum), obtained from two cross combinations, along with their four parents were sequenced by high-throughput RNA-sequencing (RNA-Seq) to find out differentially expressed gene in parent-hybrid combinations. The leaf mRNA profiles of two hybrids and their four parents were RNA-sequenced with a view to identify the potential candidate genes related to plant height heterosis. In both cross combinations, based to morphological traits mid-parent heterosis (MPH) was higher than high-parent heterosis (HPH) for plant height, leaf length, and number of flowers whereas HPH was higher than MPH for flowering time. A total of 4,327 differentially expressed genes (DEGs) were identified through RNA-Seq between the hybrids and their parents based on fold changes (fc) ≥ 2 for up-and ≤-2 for down-regulation. Venn diagram analysis revealed that there were 703 common DEGs in two hybrid combinations, those were either up-or down-regulated. Most of the commonly expressed DEGs exhibited higher non-additive effects especially overdominance (75.9%) rather than additive (19.4%) and dominance (4.76%) effects. Among the 384 functionally annotated DEGs identified through Blast2GO tool, 12 DEGs were up-regulated and 16 of them were down-regulated in a similar fashion in both hybrids as revealed by heat map analysis. These 28 universally expressed DEGs were found to encode different types of proteins and enzymes those might regulate heterosis by modulating growth, development and stress-related functions in lily. In addition, gene ontology (GO) analysis of 260 annotated DEGs revealed that biological process might play dominant role in heterotic expression. In this first report of transcriptome sequencing in Easter lily, the notable universally up-regulated DEGs annotated ABC transporter A family member-like, B3 domain-containing, disease resistance RPP13/1, auxin-responsive SAUR68-like, and vicilin-like antimicrobial peptides 2-2 proteins those were perhaps associated with plant height heterosis. The genes expressed universally due to their overdominace function perhaps influenced MPH for greater plant height-largely by modulating biological processes involved therein. The genes identified in this study might be exploited in heterosis breeding for plant height of L. longiflorum. Heterosis refers to the higher performance in any trait (s) of interest of F 1 hybrid as compared to homozygous parental lines. Charles Darwin 1 described this phenomenon for the first time ever and later Shull 2 and East 3 independently confirmed this phenomenon. Improvements in many traits of different crop and livestock species have

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confidence: 99%

Transcriptome Analysis by RNA–Seq Reveals Genes Related to Plant Height in Two Sets of Parent-hybrid Combinations in Easter lily (Lilium longiflorum)

Howlader

Robin

Natarajan

et al. 2020

Sci Rep

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“…The selection of pure lines for crossing to produce superior hybrids is one of the oldest strategies in breeding and provides a reference for the selection of progeny of new varieties (Naves et al 2021;Ren et al 2020). A wide genetic diversity and numerous genetic characters were shown in the progeny population of Lilium lancifolium × Asiatic hybrid 'Chianti' crosses, Parental morphology influences posterior phenotypic variation (Ramzan et al 2018). In cut-flower chrysanthemums, the genetics and variation of the F1 generation hybrids were analyzed after crossing.…”

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confidence: 99%

Traits and anthocyanin genetic characteristics of the progeny of crosses between white 2 azalea 'Rhododendron hybridum Hot' and pink Rhododendron pulchurum 'Wan Xia'

Jiang

Wang

Yang

et al. 2022

Preprint

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Rhododendron is a world-famous ornamental flower with an abundance of genetic resources for producing different flower color varieties through breeding. However, the lack of genetic knowledge involved mechanisms behind the flower color formation have hindered the breeding process. In this study, a cross was made between the female pink Rhododendron pulchurum 'WanXia' and the male white 'Rhododendron hybridum Hot'. A total of 61 hybrid progenies with four different flower colors including white, pink, rose and red were produced and analyzed to identify the inheritance pattern of flower color as well as their genetic diversity in the whole group. The results of the combined qualitative and quantitative trait analysis disclosed that the germplasm resources in the hybrid progeny plants were genetically enriched, which can provide an excellent germplasm for screening and breeding new Rhododendron varieties. The anthocyanin-3-O-glucoside (Cy3G) contents of hybrid progeny petals were measured using high performance liquid chromatography-mass spectrometry (HPLC-MS). The total anthocyanin (TA) contents were calculated by first developing a linear equation (y=36.52x-59.18, R2=0.998) with anthocyanin-3-O-glucoside (Cy3G) specimens. The results revealed that TA was positively and significantly correlated with the color parameter a* values as the flower color of the hybrid progeny was gradually deepened from white to red. The value of parameter L* was gradually decreased, while a*, C* and TA contents were gradually increased. L* displayed to be both significantly and negatively correlated with a*, C*and TA contents. From the trait characteristics of each progeny plants, it was observed that No.52 Pink azalea has the highest pedicel length and petal number, while No.37 Red 2 azalea has the highest corolla lobe length, corolla lobe width and TA content. Our findings indicate that it may be feasible to breed Rhododendron varieties with new flower colors based on width and TA content. In conclusion, our outcomes propose that there is probability to produce Rhododendron varieties with new flower colors by crossing and screening hybrid progenies.

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The Application of Fluorescence In Situ Hybridization in the Prescreening of Veronica Hybrids

Park,

Sevilleno,

et al. 2024

Plants

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Fluorescence in situ hybridization (FISH), a molecular cytogenetic technique that enables the visualization and identification of specific DNA sequences within chromosomes, has emerged as a pivotal tool in plant breeding programs, particularly in the case of Veronica species. Veronica, a genus with a complex reproductive system, often poses challenges in accurately identifying hybrids because of its tendency to hybridize, which leads to intricate genetic variation. This study focused on the use of FISH as a prescreening method to identify true hybrids in Veronica breeding programs. FISH analysis was first performed on the parents to identify their 45S and 5S rDNA signals, along with their respective chromosome numbers. The signals were then compared with those of the twenty progenies with reference to their supposed parents. Five true hybrids, seven self-pollinated progenies, and eight false hybrids were identified through FISH. The findings highlight the significance of FISH as a screening method that contributes significantly to the efficiency of Veronica breeding programs by ensuring the preservation of desired genetic traits and minimizing the inadvertent inclusion of misidentified hybrids. To conclude, this study underscores the vital role of FISH in enhancing the precision and success of breeding programs and opens new avenues for improved breeding strategies and crop development.

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Evaluation of F1 and BC1 hybrids of Lilium lancifolium × Asiatic hybrid ‘Chianti’ by morphological analysis and fluorescence in situ hybridization

Cited by 3 publications

References 24 publications

Transcriptome Analysis by RNA–Seq Reveals Genes Related to Plant Height in Two Sets of Parent-hybrid Combinations in Easter lily (Lilium longiflorum)

Transcriptome Analysis by RNA–Seq Reveals Genes Related to Plant Height in Two Sets of Parent-hybrid Combinations in Easter lily (Lilium longiflorum)

Traits and anthocyanin genetic characteristics of the progeny of crosses between white 2 azalea 'Rhododendron hybridum Hot' and pink Rhododendron pulchurum 'Wan Xia'

The Application of Fluorescence In Situ Hybridization in the Prescreening of Veronica Hybrids

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